A5059903124- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Cholinesterase and Neurodegenerative Diseases
- Virus-based gene therapy research
- Electrochemical sensors and biosensors
- Pluripotent Stem Cells Research
- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Higher Education and Teaching Methods
- Molecular Sensors and Ion Detection
- Flavonoids in Medical Research
- Drug Transport and Resistance Mechanisms
- Xenotransplantation and immune response
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Catalysis and Immobilization
- Education and Work Dynamics
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Computational Drug Discovery Methods
- Neuroscience and Neuropharmacology Research
- Neurogenetic and Muscular Disorders Research
- Agriculture, Soil, Plant Science
- Electrospun Nanofibers in Biomedical Applications
- Amyotrophic Lateral Sclerosis Research
- Proteoglycans and glycosaminoglycans research
Chinese Academy of Sciences
2015-2025
Guangzhou Institutes of Biomedicine and Health
2015-2025
Shenyang Institute of Automation
2015-2024
State Key Laboratory of Robotics
2024
Jining Medical University
2019-2024
University of Hong Kong
2023
Nanning Normal University
2023
Guangzhou Medical University
2018-2022
Guizhou University
2022
Hengshui University
2022
Neurodegeneration is a hallmark of many neurological diseases, including Alzheimer's, Parkinson's and the polyglutamine which are all caused by misfolded proteins that accumulate in neuronal cells brain. Although apoptosis believed to contribute neurodegeneration these cases, genetic mouse models diseases often fail replicate overt Using nuclear transfer, we generated transgenic Huntington's disease (HD) pigs express N-terminal (208 amino acids) mutant huntingtin with an expanded tract...
Abstract Cytosine base editors (CBEs) enable programmable C-to-T conversion without DNA double-stranded breaks and homology-directed repair in a variety of organisms, which exhibit great potential for agricultural biomedical applications. However, all reported cases only involved substitution at single targeted genomic site. Whether is effective multiple sites/loci has not been verified large animals. Here, by using pigs, an important animal agriculture biomedicine, as the subjective animal,...
Abstract Pigs share many physiological, biochemical, and anatomical similarities with humans have emerged as valuable large animal models for biomedical research. Considering the advantages in immune system resemblance, suitable size, longevity clinical practical monitoring purpose, SCID pigs bearing dysfunctional RAG could serve important experimental tools regenerative medicine, allograft xenograft transplantation, reconstitution experiments related to system. In this study, we report...
A highly selective long-wavelength fluorescent probe TCFB has been developed for the detection of hCE2. The can be used real-time monitoring hCE2 activity in complex biological systems.
Inbred mini-pigs are ideal organ donors for future human xenotransplantations because of their clear genetic background, high homozygosity, and inbreeding endurance. In this study, we chose fibroblast cells from a highly inbred pig line called Banna mini-pig (BMI) as donor nuclei nuclear transfer, combining with transcription activator-like effector nucleases (TALENs) successfully generated α-1,3-galactosyltransferase (GGTA1) gene biallelic knockout (KO) pigs. To validate the efficiency...
Multiple genetic modifications in pigs can essentially benefit research on agriculture, human disease and xenotransplantation. Most multi-transgenic have been produced by complex time-consuming breeding programs using multiple single-transgenic pigs. This study explored the feasibility of producing viral 2A peptide light previous indicating that it be utilized for multi-gene transfer gene therapy somatic cell reprogramming. A peptide-based double-promoter expression vector mediated four...
TAR DNA-binding protein 43 (TDP-43) is a nuclear protein, but it redistributed in the neuronal cytoplasm both amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Because small transgenic animal models often lack cytoplasmic TDP-43, how accumulation of TDP-43 contributes to these diseases remains unclear. The current study aimed at studying mechanism pathology TDP-43.We established pigs expressing mutant (M337V). This pig model shows severe phenotypes early...
Animal models are important for understanding the pathogenesis of human diseases and developing testing new drugs. Pigs have been widely used in research on cardiovascular, skin barrier, gastrointestinal, central nervous systems as well organ transplantation. Recently, pigs also become an attractive large animal model study neurodegenerative because their brains very similar to terms mass, gully pattern, vascularization, proportions gray white matters. Although adeno-associated virus type 9...
Abstract Antigen-specific T cell receptor-engineered (TCR-T) based immunotherapy has proven to be an effective method combat cancer. In recent years, cross-talk between the innate and adaptive immune systems may requisite optimize sustained antigen-specific immunity, stimulator of interferon genes (STING) is a promising therapeutic target for cancer immunotherapy. The level expression or presentation antigen in tumor cells affects recognition killing by TCR-T. This study aimed at...
Abstract Background Esophageal cancer (EC) is a global canker notorious for causing high mortality due to its relentless incidence rate, convoluted with unyielding recurrence and metastasis. However, these intricacies of EC are associated an immoderate expression NY-ESO-1 antigen, presenting lifeline adoptive T cell therapy. We hypothesized that naturally isolated higher-affinity receptors (TCRs) bind would allow lymphocytes target pronounced antitumor response efficacy. Also, targeting...