A5087057497- TiO2 Photocatalysis and Solar Cells
- Surface Modification and Superhydrophobicity
- Electrophoretic Deposition in Materials Science
- CRISPR and Genetic Engineering
- Electrohydrodynamics and Fluid Dynamics
- Energetic Materials and Combustion
- Pluripotent Stem Cells Research
- Supercapacitor Materials and Fabrication
- Advanced biosensing and bioanalysis techniques
- Graphene research and applications
- Corrosion Behavior and Inhibition
- Advanced Sensor and Energy Harvesting Materials
- Crystallization and Solubility Studies
- Concrete Corrosion and Durability
- Superconductivity in MgB2 and Alloys
- Advanced Photocatalysis Techniques
- X-ray Diffraction in Crystallography
- Copper-based nanomaterials and applications
- High-pressure geophysics and materials
- Silicon Nanostructures and Photoluminescence
- Advanced ceramic materials synthesis
- Adsorption and biosorption for pollutant removal
- Catalytic C–H Functionalization Methods
- Hydrogen embrittlement and corrosion behaviors in metals
- Boron and Carbon Nanomaterials Research
Yangtze Normal University
2017-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024-2025
Novo Nordisk Foundation
2021-2025
University of Copenhagen
2017-2025
Changjiang Institute of Survey, Planning, Design and Research
2024
Chongqing University
2014-2023
Yanshan University
2020-2023
CHN Energy (China)
2021
Sichuan University of Science and Engineering
2019-2020
Fuling Center Hospital of Chongqing
2020
Transcription activator-like effector nucleases (TALENs) are an approach for directed gene disruption and have been proved to be effective in various animal models. Here, we report that TALENs can induce somatic mutations Xenopus embryos with reliably high efficiency such heritable through germ-line transmission. We modified the Golden Gate method TALEN assembly make product suitable RNA transcription microinjection into embryos. Eight pairs of were constructed target eight Xenopu s genes,...
For the emerging amphibian genetic model Xenopus tropicalis targeted gene disruption is dependent on zinc-finger nucleases (ZFNs) or transcription activator-like effector (TALENs), which require either complex design and selection laborious construction. Thus, easy efficient genome editing tools are still highly desirable for this species. Here, we report that RNA-guided Cas9 nuclease resulted in precise all ten X. genes analyzed, with efficiencies above 45% readily up to 100%. Systematic...
These two authors Fengqin Wang and Zhaoying Shi contributed equally to this work. Competing interests The declare that they have no competing interests. Authors’ contributions FW, YS, YChen designed the work analyzed data. FW, ZS, YCui, XG carried out experiments. FW wrote manuscript. All read approved final
Abstract Direct electrochemistry, a direct electron transfer process between enzymes and electrode possesses, has important fundamental significance in bioelectrochemistry while offering very efficient electrocatalysis for enzyme‐based sensors. Herein, the pore structure of bacterial cellulose porous carbon nanofibers (BPCNFs) is tailored by controlled thermal carbonization. It discovered that rising mesopores can realize fast electrochemistry glucose oxidase (GOx) highly sensitive detection...
Mutations in SCN1A, the gene encoding α subunit of Nav1.1 channel, can cause epilepsies with wide ranges clinical phenotypes, which are associated contrasting effects channel loss-of-function or gain-of-function. In this project, CRISPR/Cas9- and TALEN-mediated genome-editing techniques were applied to induced pluripotent stem cell (iPSC)-based-disease model explore mechanism epilepsy caused by SCN1A mutation. By fluorescently labeling GABAergic subtype iPSC-derived neurons using...
Abstract Pigs share many physiological, biochemical, and anatomical similarities with humans have emerged as valuable large animal models for biomedical research. Considering the advantages in immune system resemblance, suitable size, longevity clinical practical monitoring purpose, SCID pigs bearing dysfunctional RAG could serve important experimental tools regenerative medicine, allograft xenograft transplantation, reconstitution experiments related to system. In this study, we report...
Precise genome editing is essential for both basic and translational research. The recently developed CRISPR/Cas9 system can specifically cleave a designated site of target gene to create DNA double-strand break, which triggers cellular repair mechanism either inaccurate non-homologous end joining, or site-specific homologous recombination. Unfortunately, homology-directed (HDR) challenging due its very low efficiency. Herein, we focused on improving the efficiency HDR using combination...
Abstract Ammonia is an important indicator among environmental monitoring parameters. In this work, thin-core fiber Mach-Zehnder interferometer deposited with poly (acrylic acid) (PAA), (allyamine hydrochloride) (PAH) and single-walled carbon nanotubes (SWCNTs-COOH) sensing film for the detection of ammonia gas has been presented. The modal was made by fusion splicing a small section (TCF) between two standard single mode fibers (SMF). A beam propagation method (BPM) employed design proposed...
Xenopus tropicalis is an emerging vertebrate genetic model. A gene knock-in method has not yet been reported in this species. Here, we report that heritable targeted integration can be achieved diploid frog using a concurrent cleavage strategy mediated by the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (CRISPR/Cas9) system. The key point of addition Cas9/guide RNA site donor vector, allowing simultaneous cutting chromosomal target...
High specific surface area, reasonable pore structure and heteroatom doping are beneficial to enhance charge storage, which all depend on the selection of precursors, activators preparation methods. Here, B, O N codoped biomass-derived hierarchical porous carbon was synthesized by using KCl/ZnCl2 as a combined activator porogen H3BO3 both boron source porogen. Moreover, cheap, environmentally friendly heteroatom-rich laver used precursor, impregnation freeze-drying methods were make...
Transcription activator-like effector nucleases (TALENs) are novel engineered DNA nucleases, and have been proven to be effective for gene specific targeting in various species. Recently we reported disruptions Xenopus embryos by using TALENs. Here summarize the protocol that is used our studies disruption. This covers selection of TALEN sites, assembly with a modified Golden Gate method, injection mRNAs into tropicalis embryos. We also provide details detection somatic germ line transmitted...
Zinc-finger nucleases and transcription activator-like effector are novel gene-editing platforms contributing to redefine the boundaries of modern biological research. They composed a non-specific cleavage domain tailor made DNA-binding module, which enables broad range genetic modifications by inducing efficient DNA double-strand breaks at desired loci. Among other remarkable uses, these have been employed produce gene knockouts in mid-size large animals, such as rabbits pigs, respectively....