A5063441710- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- CRISPR and Genetic Engineering
- Fungal and yeast genetics research
- Plant biochemistry and biosynthesis
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Microbial Natural Products and Biosynthesis
- Biosensors and Analytical Detection
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Bioeconomy and Sustainability Development
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- Fermentation and Sensory Analysis
- Transgenic Plants and Applications
- Gene Regulatory Network Analysis
- Catalysis for Biomass Conversion
- Nail Diseases and Treatments
- Chemical Synthesis and Analysis
- Biochemical and Molecular Research
- Molecular Biology Techniques and Applications
Beijing University of Chemical Technology
2017-2025
Chalmers University of Technology
2011-2023
Bioprocess Control (Sweden)
2023
Beijing Advanced Sciences and Innovation Center
2023
Agency for Science, Technology and Research
2015-2019
Institute of Chemical and Engineering Sciences
2014
Tianjin University
2006-2013
Abstract With rapid progress in DNA synthesis and sequencing, strain engineering starts to be the rate-limiting step synthetic biology. Here, we report a gRNA-tRNA array for CRISPR-Cas9 (GTR-CRISPR) multiplexed of Saccharomyces cerevisiae . Using reported gRNAs shown effective, this system enables simultaneous disruption 8 genes with 87% efficiency. We further an accelerated Lightning GTR-CRISPR that avoids cloning Escherichia coli by directly transforming Golden Gate reaction mix yeast....
ABSTRACT Acetyl coenzyme A (acetyl-CoA) carboxylase (ACCase) plays a central role in carbon metabolism and has been the site of action for development therapeutics or herbicides, as its product, malonyl-CoA, is precursor production fatty acids other compounds. Control Acc1 activity yeast Saccharomyces cerevisiae occurs mainly at two levels, i.e., regulation transcription repression by Snf1 protein kinase level. Here, we demonstrate strategy improving ACCase S. abolishing posttranslational...
Biorefinery production of fuels and chemicals represents an attractive route for solving current energy crisis, as well reducing green-house gas (GHG) emissions from ships, planes, long-haul trucks. The biorefinery industry is under transition the use food (1 G, 1st generation), to biomass (2 2nd generation). Moreover, atmospheric CO2 (3 3rd generation) has caught increased attention possible next-generation biorefinery. Here we discuss how microorganisms can be engineered CO2-based...
Due to an increasing demand of transportation fuels, a lower availability cheap crude oil and lack sustainability fossil gradual shift from petroleum based fuels towards alternative renewable fuel resources will be required in the near future. Fatty acid ethyl esters (FAEEs) have properties similar current diesel could therefore form important contribution development sustainable It is develop novel cell factories for efficient production FAEEs their precursors. Here, Saccharomyces...
Wax ester synthases (WSs) can synthesize wax esters from alcohols and fatty acyl coenzyme A thioesters. The knowledge of the preferred substrates for each WS allows use yeast cells production that are high-value materials be used in a variety industrial applications. products WSs include acid ethyl esters, which directly as biodiesel.Here, heterologous derived five different organisms were successfully expressed evaluated their substrate preference Saccharomyces cerevisiae. We investigated...
Microbial production of fatty acid derived chemicals and fuels is currently great interest due to the limited resources increasing prices petroleum petroleum-based products. The development Saccharomyces cerevisiae as a ethyl ester (FAEE) cell factory would represent an opportunity for biodiesel its successful history in biotechnology area. However, (FA) biosynthesis highly regulated usually not high enough developing efficient process. In S. cerevisiae, FAs are degraded by β-oxidation large...
Although many prokaryotes have glycolysis alternatives, it's considered as the only energy-generating glucose catabolic pathway in eukaryotes. Here, we managed to create a hybrid-glycolysis yeast. Subsequently, identified an inositol pyrophosphatase encoded by OCA5 that could regulate and respiration adjusting 5-diphosphoinositol 1,2,3,4,6-pentakisphosphate (5-InsP7) levels. 5-InsP7 levels expression of genes involved respiration, representing global mechanism sense ATP central carbon...
Abstract CO 2 fixation plays a key role to make biobased production cost competitive. Here, we use 3-hydroxypropionic acid (3-HP) showcase how enables approaching theoretical-yield production. Using genome-scale metabolic models calculate the envelope, demonstrate that provision of bicarbonate, formed from , restricts previous attempts for high yield 3-HP. We thus develop multiple strategies bicarbonate uptake, including identification Sul1 as potential transporter, domain swapping...
Wax ester synthases (WSs) can synthesize wax esters from alcohols and fatty acyl coenzyme A thioesters. The knowledge of the preferred substrates for each WS allows use yeast cells production that are high-value materials be used in a variety industrial applications. products WSs include acid ethyl esters, which directly as biodiesel. Here, heterologous derived five different organisms were successfully expressed evaluated their substrate preference Saccharomyces cerevisiae. We investigated...
ABSTRACT In recent years, significant advances have been made to engineer robust microbes for overproducing biochemical products from renewable resources. These accomplishments a large extend based on plasmid methods. However, maintenance may cause metabolic burden the host cell and plasmid‐based overexpression of genes can result in genetically unstable strains, which contributes loss productivity. Here, chromosome engineering method delta integration was applied Saccharomyces cerevisiae...
Abstract n -Butanol has several favourable properties as an advanced fuel or a platform chemical. Bio-based production of -butanol is becoming increasingly important for sustainable chemical industry. Synthesis can be achieved via more than one metabolic pathway. Here we report the engineering Saccharomyces cerevisiae to produce through synergistic pathway: endogenous threonine pathway and introduced citramalate Firstly, characterized optimized pathway; then, synthase (CimA) mediated was...
Propionate is widely used as a preservative in the food and animal feed industries. currently produced by petrochemical processes, fermentative production of propionate remains challenging.In this study, synthetic pathway was constructed budding yeast Saccharomyces cerevisiae, for under aerobic conditions. Through expression tdcB aldH from Escherichia coli kivD Lactococcus lactis, L-threonine converted to via 2-ketobutyrate propionaldehyde. The resulting aerobically 0.21 g L-1 glucose shake...
Abstract Synthetic biology requires efficient systems that support the well-coordinated co-expression of multiple genes. Here, we discover a 9-bp nucleotide sequence enables polycistronic gene expression in yeasts and filamentous fungi. Coupling to multiplexed, markerless, CRISPR/Cas9-based genome editing, develop strategy termed HACKing (Highly Accessible system by CracKing genes into genome) for assembly multigene pathways. allows level each enzyme be precalibrated linking their...