A5100775467- Microbial Metabolic Engineering and Bioproduction
- Reproductive Biology and Fertility
- Biofuel production and bioconversion
- Sperm and Testicular Function
- Enzyme Catalysis and Immobilization
- Bacterial Genetics and Biotechnology
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Ovarian function and disorders
- Enzyme Structure and Function
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Renal and related cancers
- Angiogenesis and VEGF in Cancer
- Reproductive Physiology in Livestock
- Photosynthetic Processes and Mechanisms
- Bioinformatics and Genomic Networks
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Cancer Cells and Metastasis
- Ferroptosis and cancer prognosis
- Biochemical and Molecular Research
- Cancer, Hypoxia, and Metabolism
- Algal biology and biofuel production
Institute of Animal Sciences
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Xinjiang Academy of Agricultural and Reclamation Science
2024-2025
Zhejiang Chinese Medical University
2025
Shanxi Datong University
2025
Tianjin Medical University
2008-2024
Shanxi Medical University
2010-2024
United States Air Force
2024
VA Palo Alto Health Care System
2006-2024
Tianjin Research Institute of Electric Science (China)
2024
Vitrification of oocytes has been shown to be closely associated with increased levels reactive oxygen species (ROS) and apoptotic events. However, little information is available the effect melatonin on ROS events in vitrified oocytes. Therefore, we studied bovine by supplementing vitrification solution or vitro maturation (IVM) 10(-9) m melatonin. We analyzed ROS, mitochondrial Ca(2+) (mCa(2+) ) membrane potential (ΔΨm), externalization phosphatidylserine (PS), caspase-3 activation, DNA...
Melatonin is a well-characterized antioxidant that has been successfully used to protect oocytes from reactive oxygen species during in vitro maturation (IVM), resulting improved fertilization capacity and development ability. However, the mechanism via which melatonin improves oocyte ability remains be determined. Here, we studied effects of on cytoplasmic bovine oocytes. In present study, were cultured IVM medium supplemented with 0, 10-7 , 10-9 10-11 mol/L melatonin, parameters MII after...
Riboflavin (vitamin B2), the precursor of flavin cofactors mononucleotide (FMN) and adenine dinucleotide (FAD), is used commercially as an animal feed supplement food colorant. E. coli a robust host for various genetic manipulations has been employed efficient production biofuels, polymers, amino acids, bulk chemicals. Thus, aim this study was to understand metabolic capacity riboflavin by modification central metabolism, biosynthesis pathway optimization fermentation conditions. The basic...
Cryodamage is a major problem in semen cryopreservation, causing changes the levels of proteins that influence function and motility spermatozoa. In this study, protein samples prepared from fresh frozen-thawed boar spermatozoa were compared using isobaric tags for relative absolute quantification (iTRAQ) labeling technique coupled to 2D LC-MS/MS analysis. A total 41 differentially expressed identified quantified, including 35 present at higher six lower by least mean 1.79-fold (P<0.05). On...
2,3-Butanediol (2,3-BD) with low toxicity to microbes, could be a promising alternative for biofuel production. However, most of the 2,3-BD producers are opportunistic pathogens that not suitable industrial-scale fermentation. In our previous study, wild-type Bacillus subtilis 168, as class I microorganism, was first found generate only d-(-)-2,3-BD (purity >99 %) under oxygen conditions.In this work, B. engineered produce chiral pure meso-2,3-BD. First, production abolished by deleting...
Extreme temperature during summer may lead to heat stress in cattle and compromise their productivity. It also poses detrimental impacts on the developmental capacity of bovine budding oocytes, which halt fertility. To mitigate adverse effects stress, it is necessary investigate mechanisms through affects oocytes. The primary goal this study was impact epigenetic modifications oocytes embryos, as well oocyte capacity, reactive oxygen species, mitochondrial membrane potential, apoptosis,...
Abstract Motivation: Metabolic networks are organized in a modular, hierarchical manner. Methods for rational decomposition of the metabolic network into relatively independent functional subsets essential to better understand modularity and organization principle large-scale, genome-wide network. Network is also necessary analysis metabolism by pathway methods that often hampered problem combinatorial explosion due complexity Decomposition proposed literature mainly based on connection...
<b>Objectives:</b> To identify peripheral blood autoantibody and cytokine profiles that characterise clinically relevant subgroups of patients with early rheumatoid arthritis using antigen microarrays a multiplex assay. <b>Methods:</b> Serum samples from 56 diagnosis <6 months' duration were tested. Cytokine also determined in psoriatic (PsA) ankylosing spondylitis (n = 21), healthy individuals 19). Data analysed Kruskal–Wallis test Dunn's adjustment for multiple comparisons, linear...
Abstract Background Direct in vivo investigation of human metabolism is complicated by the distinct metabolic functions various sub-cellular organelles. Diverse micro-environments different organelles may lead to same protein and use enzymes for reaction. To better understand complexity metabolism, a compartmentalized network with integrated location information required. Results We extended previously reconstructed Edinburgh Human Metabolic Network (EHMN) [Ma, et al. Molecular Systems...
Promoters are among the most-important and most-basic tools for control of metabolic pathways. However, previous research mainly focused on screening characterization some native promoters in Bacillus subtilis. To develop a broadly applicable promoter system this important platform organism, we created de novo synthetic library (SPL) based consensus sequences by analyzing microarray transcriptome data B. subtilis 168. A total 214 potential spanning gradient strengths was isolated...
5-Aminolevulinic acid (5-ALA) has recently attracted attention for its potential applications in the fields of medicine and agriculture. In this study, Corynebacterium glutamicum was firstly engineered 5-ALA production via C4 pathway. HemA encoding 5-aminolevulinic synthase from Rhodobacter sphaeroides codon optimized expressed C. ATCC13032, resulting accumulation 5-ALA. Deletion all known genes responsible formation acetate lactate further enhanced Overexpression ppc gene phoenolpyruvate...
Purine nucleotides are essential metabolites for living organisms because they involved in many important processes, such as nucleic acid synthesis, energy supply, and biosynthesis of several amino acids riboflavin. Owing to the pivotal roles purines cell physiology, pool intracellular purine must be maintained under strict control, hence de novo biosynthetic pathway is tightly regulated by transcription repression inhibition mechanism. Deregulation this engineering Bacillus subtilis. was...
Abstract Background Metabolic engineering has expanded from a focus on designs requiring small number of genetic modifications to increasingly complex driven by advances in multiplex genome editing technologies. However, simultaneously modulating multiple genes the chromosome remains challenging Bacillus subtilis . Thus, developing an efficient and convenient method for B. is imperative. Results Here, we developed CRISPR/Cas9n-based system iterative This enabled us introduce various types...
We aimed to investigate the effect of melatonin on bovine frozen-thawed semen and its impact fertilization outcome. Plasma membrane integrity, mitochondrial activity, acrosome levels intracellular reactive oxygen species (ROS) were measured in spermatozoa treated with different concentrations melatonin. Melatonin-treated then used for vitro fertilization, followed by analysis subsequent embryo development expression apoptosis- antioxidant-related genes. The results revealed that 10-5 10-3 M...
Paraquat (PQ), a broad-spectrum agricultural pesticide, causes cellular toxicity by increasing oxidative stress levels in various biological systems, including the reproductive system. PQ exposure embryotoxicity and reduces developmental abilities of embryos. However, there is little information regarding toxic effects on oocyte maturation. In this study, we studied melatonin PQ-induced damage bovine oocytes. disrupted nuclear cytoplasmic maturation, which was manifested as decreased cumulus...
Sperm motility is the main index used to assess quality of bull semen. It may also be evaluate fertility potential bulls. Protein-coding mRNA and long noncoding RNA (lncRNA) participate in regulation spermatogenesis. Here, we employed strand-specific sequencing profile semen transcriptome (mRNA lncRNA) six paired full-sibling Holstein bulls with divergent sperm determine functions lncRNA motility. Among 20,875 protein-encoding genes detected semen, 19 were differentially expressed between...
2,3-Butanediol is an important compound that can be used in many areas, especially as a platform chemical and liquid fuel. But traditional 2,3-butanediol producing microorganisms, such Klebsiella pneumonia K. xoytoca, are pathogens they only ferment sugars at 37°C. Here, we reported newly developed Bacillus licheniformis. A protoplast transformation system was optimized for this organism. With method, marker-less gene deletion protocol successfully to knock out the ldh of B. licheniformis...