A5026886431- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Plant biochemistry and biosynthesis
- CRISPR and Genetic Engineering
- Lignin and Wood Chemistry
- Microbial Natural Products and Biosynthesis
- Plant tissue culture and regeneration
- RNA and protein synthesis mechanisms
- Biochemical and biochemical processes
- Enzyme Catalysis and Immobilization
- Fermentation and Sensory Analysis
- Catalysis for Biomass Conversion
- Cancer Treatment and Pharmacology
- Enzyme-mediated dye degradation
- Algal biology and biofuel production
- Fungal Biology and Applications
- Bacterial Genetics and Biotechnology
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Microbial metabolism and enzyme function
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Bacteriophages and microbial interactions
- Genomics and Chromatin Dynamics
Tianjin University
2016-2025
Ministry of Education of the People's Republic of China
2011-2024
Chengdu University
2024
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2013-2022
Tianjin International Joint Academy of Biomedicine
2017
Anhui Agricultural University
2014-2015
Michigan State University
2010
Samjin Pharm (South Korea)
2005
Nankai University
2003
Biofoundries provide an integrated infrastructure to enable the rapid design, construction, and testing of genetically reprogrammed organisms for biotechnology applications research. Many biofoundries are being built a Global Biofoundry Alliance has recently been established coordinate activities worldwide.
INTRODUCTION The Saccharomyces cerevisiae 2.0 project (Sc2.0) aims to modify the yeast genome with a series of densely spaced designer changes. Both synthetic chromosome arm (synIXR) and entirely (synIII) function high fitness in yeast. For synthesis projects, precise engineering physical sequence match specified design is important for systematic evaluation underlying principles. Yeast can maintain nuclear chromosomes as rings, occurring by chance at repeated sequences, although cyclized...
INTRODUCTION Design and construction of an extensively modified yeast genome is a direct means to interrogate the integrity, comprehensiveness, accuracy knowledge amassed by community date. The international synthetic project (Sc2.0) aims build entirely designer, Saccharomyces cerevisiae genome. designed increase stability genetic flexibility while maintaining cell fitness near that wild type. A major challenge for synthesis lies in identifying eliminating fitness-reducing sequence variants...
INTRODUCTION It has long been an interesting question whether a living cell can be constructed from scratch in the lab, goal that may not realized anytime soon. Nonetheless, with advances DNA synthesis technology, complete genetic material of organism now synthesized chemically. Hitherto, genomes several organisms including viruses, phages, and bacteria have designed constructed. These synthetic are able to direct all normal biological functions, capable self-replication production...
Lignin-derived phenolic compounds are universal in the hydrolysate of pretreated lignocellulosic biomass. The phenolics reduce efficiency enzymatic hydrolysis and increase cost ethanol production. We investigated inhibition on cellulase during using vanillin as one typical lignin-derived Avicel cellulose substrate.As concentration increased from 0 to 10 mg/mL, conversion after 72-h decreased 53 26 %. Enzyme deactivation precipitation were detected with addition. enzyme activity consecutively...
Poly(ethylene terephthalate) hydrolase (PETase) from Ideonella sakaiensis exhibits a strong ability to degrade poly(ethylene (PET) at room temperature, and is thus regarded as potential tool solve the issue of polyester plastic pollution. Therefore, we explored interaction between PETase substrate (a dimer PET monomer ethylene terephthalate, 2PET), using model its substrate. In this study, focused on six key residues around substrate-binding groove in order create novel high-efficiency...
INTRODUCTION Although much effort has been devoted to studying yeast in the past few decades, our understanding of this model organism is still limited. Rapidly developing DNA synthesis techniques have made a “build-to-understand” approach feasible reengineer on genome scale. Here, we report completion 770-kilobase synthetic chromosome II (synII). SynII was characterized using extensive Trans-Omics tests. Despite considerable sequence alterations, synII virtually indistinguishable from wild...
Microbial production of lycopene, a commercially and medically important compound, has received increasing concern in recent years. Saccharomyces cerevisiae is regarded as safer host for lycopene than Escherichia coli. However, to date, the yield (mg/g DCW) S. was lower that E. coli did not facilitate downstream extraction process, which might be attributed incompatibility between cell heterologous pathway. Therefore, achieve overproduction cerevisiae, both pathway should delicately...
Vanillin, known as the "queen of flavors", is an extensively used and important aromatic compound with multiple functions, including aldehyde, ether, hydroxyl. Converting lignocellulosic biomass-derived substrates to natural vanillin sustainable economical through artificial cell factories. However, inefficiency exogenous enzymes toxic effect on cells limit its production. In this study, pathway reconstruction integration site optimization Saccharomyces cerevisiae enabled production 363.0...
Compatibility between host cells and heterologous pathways is a challenge for constructing organisms with high productivity or gain of function. Designer yeast incorporating the Synthetic Chromosome Rearrangement Modification by LoxP-mediated Evolution (SCRaMbLE) system provide platform generating genotype diversity. Here we construct genetic AND gate to enable precise control SCRaMbLE method generate synthetic haploid diploid desired phenotypes. The yield carotenoids increased 1.5-fold...
Simultaneous saccharification and fermentation (SSF) is a promising process for bioconversion of lignocellulosic biomass. High glucan loading hydrolysis an efficient approach to reduce the capital costs bio-based products production. The SSF steam-exploded corn stover (SECS) ethanol production at high temperature was investigated in this study. Glucan conversion biomass pretreated by steam explosion maintained approximately 71 79% enzyme 30 filter paper units (FPU)/g glucan, 74 82% 60 FPU/g...
Although the design of synthetic yeast genome Sc2.0 is highly conservative with respect to gene content, deletion several classes repeated sequences and introduction thousands designer changes may affect organization potentially alter cellular functions. We report here Hi-C-determined three-dimensional (3D) conformations chromosomes. The absence repeats leads a smoother contact pattern more precisely tractable chromosome conformations, large-scale genomic globally unaffected by presence...
A synbio strategy for efficient sugar-to-electricity conversion.