A5050908580- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Biodiesel Production and Applications
- Hydrocarbon exploration and reservoir analysis
- Plant Reproductive Biology
- Microbial Metabolic Engineering and Bioproduction
- Biocrusts and Microbial Ecology
- Plant nutrient uptake and metabolism
- Redox biology and oxidative stress
- Coronary Artery Anomalies
- Mechanical Circulatory Support Devices
- Cardiac Structural Anomalies and Repair
- Microbial Community Ecology and Physiology
- CRISPR and Genetic Engineering
- Kawasaki Disease and Coronary Complications
- Enzyme Catalysis and Immobilization
- Chromosomal and Genetic Variations
- Plant Micronutrient Interactions and Effects
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Metabolomics and Mass Spectrometry Studies
- Cardiac Arrhythmias and Treatments
- Fungal and yeast genetics research
Chinese Academy of Agricultural Sciences
2017-2025
Institute of Agricultural Resources and Regional Planning
2017-2025
Guangdong Institute for Drug Control
2024-2025
Tsinghua University
2014-2024
Fudan University
2023-2024
Linyi People's Hospital
2024
Linyi University
2024
Beijing Hua Xin Hospital
2009-2023
State Key Laboratory of Genetic Engineering
2023
Pudong Medical Center
2023
Abstract An integrated approach of biodiesel production from heterotrophic Chlorella protothecoides focused on scaling up fermentation in bioreactors was reported this study. Through substrate feeding and process controls, the cell density C. achieved 15.5 g L −1 5 L, 12.8 750 14.2 11,000 bioreactors, respectively. Resulted metabolism, lipid content reached 46.1%, 48.7%, 44.3% dry weight samples Transesterification microalgal oil catalyzed by immobilized lipase Candidia sp. 99–125. With 75%...
INTRODUCTION It has long been an interesting question whether a living cell can be constructed from scratch in the lab, goal that may not realized anytime soon. Nonetheless, with advances DNA synthesis technology, complete genetic material of organism now synthesized chemically. Hitherto, genomes several organisms including viruses, phages, and bacteria have designed constructed. These synthetic are able to direct all normal biological functions, capable self-replication production...
Abstract BACKGROUND: As a potential source of biomass, Jerusalem artichoke has been studied for bioethanol production; however, thus far it not investigated the production other liquid biofuels, such as biodiesel. This work aims to develop novel approach biodiesel from tuber using heterotrophic microalgae. RESULTS: In this study, Chlorella protothecoides utilized hydrolysate carbon and accumulated lipid in vivo , with content high 44% by dry mass, conversion ratio about 25% 4‐day scale...
Microalgae-derived biodiesel is a promising substitute for conventional fossil fuels. In particular, the green alga Chlorella protothecoides sp. 0710 regarded as one of best candidates commercial manufacture microalgae-derived biofuel. This due not only to its ability live autotrophically through photosynthesis, but also capacity produce large amount biomass and lipid fermentation glucose. However, until present study, neither genome sequence nor platform required molecular manipulations...
Global environmental temperature changes threaten innumerable plant species. Although various signaling networks regulate responses to fluctuations, the mechanisms unifying these diverse processes are largely unknown. Here, we demonstrate that an Arabidopsis monothiol glutaredoxin, AtGRXS17 (At4g04950), plays a critical role in redox homeostasis and hormone perception mediate temperature-dependent postembryonic growth. expression was induced by elevated temperatures. Lines altered were...
Abstract SCRaMbLE is a novel system implemented in the synthetic yeast genome, enabling massive chromosome rearrangements to produce strains with large genotypic diversity upon induction. Here we describe reporter of SCRaMbLEd cells using efficient selection, termed ReSCuES, based on loxP-mediated switch two auxotrophic markers. We show that all randomly isolated clones contained within chromosome, demonstrating high efficiency selection. Using illustrate ability generate increased tolerance...
It is a routine task in metabolic engineering to introduce multicomponent pathways into heterologous host for production of metabolites. However, this process sometimes may take weeks months due the lack standardized genetic tools. Here, we present method design and construction biological parts based on native genes regulatory elements Saccharomyces cerevisiae. We have developed highly efficient protocols (termed YeastFab Assembly) synthesize these as parts, which can be used assemble...
Heterotrimeric G proteins are important transducers of receptor signaling, functioning in plants with CLAVATA receptors controlling shoot meristem size and pathogen-associated molecular pattern basal immunity. However, whether specific members the heterotrimeric complex potentiate cross-talk between development defense, extent to which these functions conserved across species, have not yet been addressed. Here we used CRISPR/Cas9 knock out maize protein β subunit gene (Gβ) found that mutants...
Meristems contain groups of indeterminate stem cells, which are maintained by a feedback loop between CLAVATA (CLV) and WUSCHEL (WUS) signaling. CLV signaling involves the secretion CLV3 peptide its perception number Leucine-Rich-Repeat (LRR) receptors, including receptor-like kinase CLV1 protein CLV2 coupled with CORYNE (CRN) pseudokinase. CLV2, maize ortholog FASCIATED EAR2 (FEA2) appear to function in several related CLV3/EMBRYO-SURROUNDING REGION (CLE) ligands. Nevertheless, how...
Summary The integration of haploid induction and genome editing, termed HI‐Edit/IMGE, is a promising tool for generating targeted mutations crop breeding. However, the technical components stacking suitable maize seed industry have yet to be fully characterised tested. Here, we developed assessed three HI‐Edit/IMGE lines: Edit Wx , Sh & using inducer CHOI3 lines engineered CRISPR‐Cas9 system targeting Waxy1 ( Wx1 ) Shrunken2 Sh2 genes. We meticulously systems, focusing on copy numbers...