A5023154943- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- RNA Research and Splicing
- Genetic factors in colorectal cancer
- Chromosomal and Genetic Variations
- Microtubule and mitosis dynamics
- Fungal and yeast genetics research
Synthetic Genomics (United States)
2022
Chinese Academy of Sciences
2022
Shenzhen Institutes of Advanced Technology
2018-2019
Tsinghua University
2017-2019
INTRODUCTION It has long been an interesting question whether a living cell can be constructed from scratch in the lab, goal that may not realized anytime soon. Nonetheless, with advances DNA synthesis technology, complete genetic material of organism now synthesized chemically. Hitherto, genomes several organisms including viruses, phages, and bacteria have designed constructed. These synthetic are able to direct all normal biological functions, capable self-replication production...
Summary Whether synthetic genomes can power life has attracted broad interest in the biology field, especially when are extensively modified with thousands of designer features. Here we report de novo synthesis largest eukaryotic chromosome thus far, synIV , a 1,454,621-bp Saccharomyces cerevisiae resulting from extensive genome streamlining and modification. During construction developed megachunk assembly combined hierarchical integration strategy, which significantly increased accuracy...
Proliferating cell nuclear antigen (PCNA), a ring‐shaped homotrimer, is an essential factor in DNA metabolism including replication and repair. PCNA functions these reactions appear to be regulated by posttranslational modifications, such as ubiquitylation, sumoylation phosphorylation . Recently, we have shown that EGFR at tyrosine 211 (Y211) disrupts interactions with mismatch recognition proteins, thereby inhibiting repair (MMR). In addition, phosphorylated promotes misincorporation during...
Abstract Proliferating cell nuclear antigen (PCNA), encoded by POL30 in Saccharomyces cerevisiae , is a key component of DNA metabolism. Here library consisting 308 PCNA mutants was designed and synthesized to probe the contribution each residue its biological function. Five regions were identified with elevated sensitivity damaging reagents using high-throughput phenotype screening. Using series genetic biochemical analyses, we demonstrated that one particular mutant, K168A, which displayed...