Jianhui Gong
A5045161248- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Pluripotent Stem Cells Research
- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
- Microbial Community Ecology and Physiology
- Inorganic Fluorides and Related Compounds
- Mosquito-borne diseases and control
- Genetics, Aging, and Longevity in Model Organisms
- Molecular Spectroscopy and Structure
- Crystallography and molecular interactions
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- DNA and Biological Computing
- RNA modifications and cancer
- Microbial Metabolic Engineering and Bioproduction
BGI Group (China)
2015-2023
BGI Research
2022
China National GeneBank
2017-2020
Yantai University
2011
INTRODUCTION It has long been an interesting question whether a living cell can be constructed from scratch in the lab, goal that may not realized anytime soon. Nonetheless, with advances DNA synthesis technology, complete genetic material of organism now synthesized chemically. Hitherto, genomes several organisms including viruses, phages, and bacteria have designed constructed. These synthetic are able to direct all normal biological functions, capable self-replication production...
INTRODUCTION Although much effort has been devoted to studying yeast in the past few decades, our understanding of this model organism is still limited. Rapidly developing DNA synthesis techniques have made a “build-to-understand” approach feasible reengineer on genome scale. Here, we report completion 770-kilobase synthetic chromosome II (synII). SynII was characterized using extensive Trans-Omics tests. Despite considerable sequence alterations, synII virtually indistinguishable from wild...
Synthetic chromosome rearrangement and modification by loxP -mediated evolution (SCRaMbLE) generates combinatorial genomic diversity through rearrangements at designed recombinase sites. We applied SCRaMbLE to yeast synthetic arm synIXR (43 sites) then used a computational pipeline infer or unscramble the sequence of recombinations that created observed genomes. Deep sequencing 64 strains revealed 156 deletions, 89 inversions, 94 duplications, 55 additional complex rearrangements; several...
Aneuploidy compromises genomic stability, often leading to embryo inviability, and is frequently associated with tumorigenesis aging. Different aneuploid chromosome stoichiometries lead distinct transcriptomic phenotypic changes, making it helpful study aneuploidy in tightly controlled genetic backgrounds. By deploying the engineered SCRaMbLE (synthetic rearrangement modification by loxP-mediated evolution) system newly synthesized megabase Sc2.0 VII (synVII), we constructed a synthetic...
Range of DNA repair in response to double-strand breaks induced human preimplantation embryos remains uncertain due the complexity analyzing single- or few-cell samples. Sequencing such minute input requires a whole genome amplification that can introduce artifacts, including coverage nonuniformity, biases, and allelic dropouts at target site. We show here that, on average, 26.6% preexisting heterozygous loci control single blastomere samples appear as homozygous after indicative dropouts....
Abstract Applications of genome editing ultimately depend on DNA repair triggered by targeted double-strand breaks (DSBs). However, mechanisms in human cells remain poorly understood and vary across different cell types. Here we report that DSBs selectively induced a mutant allele heterozygous embryos are repaired gene conversion using an intact wildtype homolog as template up to 40% embryos. We also show targeting homozygous loci facilitates interplay non-homologous end joining (NHEJ)...
Abstract The forfeiting of photosynthetic capabilities has occurred independently many times throughout eukaryotic evolution. But almost all non-photosynthetic plants and algae still retain a colorless plastid an associated genome, which performs fundamental processes apart from photosynthesis. Unfortunately, little is known about the forces leading to loss; this largely because there lack data transitional species. Here, we compare genomes two “transitional” green algae: photosynthetic,...
Abstract Aneuploidy compromises genomic stability, often leading to embryo inviability, and is frequently associated with tumorigenesis aging. Different aneuploid chromosome stoichiometries lead distinct transcriptomic phenotypic changes, making it helpful study aneuploidy in tightly controlled genetic backgrounds. By deploying the engineered SCRaMbLE system newly synthesized Sc2.0 megabase VII ( synVII ), we constructed a synthetic disomic yeast screened hundreds of SCRaMbLEd derivatives...
Abstract The NH 2 Br‐HOX (X = F, Cl, and Br) complexes have been investigated with quantum chemical calculations at the MP2/aug‐cc‐pVTZ level. Five isomers are observed for Cl Br complexes, whereas only two found F complex. geometrical, energetic, spectroscopic parameters analyzed these complexes. hydrogen‐bonded more stable than halogen‐bonded ones. In most associated OH OX bonds elongated show a red shift, distant contracted exhibit blue shift. natural bond orbital atoms in molecules. ©...
Using a comprehensive library of histone H2A and H2B mutants, we assessed the biological function each amino acid residue involved in various stress conditions including exposure to different DNA damage-inducing reagents, growth temperatures, other chemicals. N- C-termini were critical for maintaining nucleosome mutations these regions led pleiotropic phenotypes. Additionally, two screens performed using this library, monitoring heterochromatin gene silencing genome stability, identify...