A5021389606- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Fermentation and Sensory Analysis
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
University of Manchester
2015-2023
Manchester Academic Health Science Centre
2018
Here, we report the design, construction, and characterization of a tRNA neochromosome, designer chromosome that functions as an additional, de novo counterpart to native complement Saccharomyces cerevisiae. Intending address one central design principles Sc2.0 project, ∼190-kb neochromosome houses all 275 relocated nuclear genes. To maximize stability, incorporates orthogonal genetic elements from non-S. cerevisiae yeast species. Furthermore, presence 283 rox recombination sites enables...
Aneuploidy compromises genomic stability, often leading to embryo inviability, and is frequently associated with tumorigenesis aging. Different aneuploid chromosome stoichiometries lead distinct transcriptomic phenotypic changes, making it helpful study aneuploidy in tightly controlled genetic backgrounds. By deploying the engineered SCRaMbLE (synthetic rearrangement modification by loxP-mediated evolution) system newly synthesized megabase Sc2.0 VII (synVII), we constructed a synthetic...
The fermentation of sugars to alcohols by microbial systems underpins many biofuel initiatives. Short chain alcohols, like n-butanol, isobutanol and isopropanol, offer significant advantages over ethanol in terms fuel attributes. However, production from resistant Saccharomyces cerevisiae strains is significantly less complicated than for these alternative alcohols.In this study, we have transplanted an n-butanol synthesis pathway largely Clostridial sp. the genome S. strain. Production only...
n-Butanol represents a key commodity chemical and holds significant potential as biofuel. It can be produced naturally by Clostridia species via the ABE pathway. However, butanol production such systems associated with drawbacks. Therefore, substantial efforts have been made toward engineering suitable industrial host for production. For instance, we previously generated metabolically engineered Saccharomyces cerevisiae strain that produces ~300 mg/L from combined endogenous exogenous...
Abstract Aneuploidy compromises genomic stability, often leading to embryo inviability, and is frequently associated with tumorigenesis aging. Different aneuploid chromosome stoichiometries lead distinct transcriptomic phenotypic changes, making it helpful study aneuploidy in tightly controlled genetic backgrounds. By deploying the engineered SCRaMbLE system newly synthesized Sc2.0 megabase VII ( synVII ), we constructed a synthetic disomic yeast screened hundreds of SCRaMbLEd derivatives...
Recent advances in synthetic genomics launched the ambitious goal of generating first designer eukaryote, based on model organism Saccharomyces cerevisiae (Sc2.0). Excitingly, Sc2.0 project is now nearing its completion and SCRaMbLE, an accelerated evolution tool implemented by integration symmetrical loxP sites (loxPSym) downstream almost every non-essential gene, arguably most applicable genome-wide alteration to date. The SCRaMbLE system offers capability perform rapid genome...
Abstract Here we report the design, construction and characterization of a tRNA neochromosome, designer chromosome that functions as an additional, de novo counterpart to native complement Saccharomyces cerevisiae . Intending address one central design principles Sc2.0 project, ∼190 kb neochromosome houses all 275 relocated nuclear genes. To maximize stability, incorporated orthogonal genetic elements from non- S. yeast species. Furthermore, presence 283 rox recombination sites enable...