A5039701148- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Advanced biosensing and bioanalysis techniques
- Gas Sensing Nanomaterials and Sensors
- RNA Interference and Gene Delivery
- Real-time simulation and control systems
- Force Microscopy Techniques and Applications
- Genomics and Phylogenetic Studies
- Automotive and Human Injury Biomechanics
- Environmental Sustainability in Business
- Gut microbiota and health
- Chromosomal and Genetic Variations
- Modeling, Simulation, and Optimization
- Viral gastroenteritis research and epidemiology
- Aquaculture disease management and microbiota
- Acoustic Wave Resonator Technologies
- Microbial Metabolic Engineering and Bioproduction
- Medical Research and Treatments
- Advanced MEMS and NEMS Technologies
- Forecasting Techniques and Applications
- Vehicle Dynamics and Control Systems
- Environmental and Sediment Control
- Plant biochemistry and biosynthesis
- Probiotics and Fermented Foods
National Central University
2021
National Taiwan University of Science and Technology
2015-2020
National Sun Yat-sen University
2019
Shanghai Jiao Tong University
2007-2010
I-Shou University
2005
INTRODUCTION It has long been an interesting question whether a living cell can be constructed from scratch in the lab, goal that may not realized anytime soon. Nonetheless, with advances DNA synthesis technology, complete genetic material of organism now synthesized chemically. Hitherto, genomes several organisms including viruses, phages, and bacteria have designed constructed. These synthetic are able to direct all normal biological functions, capable self-replication production...
It is a routine task in metabolic engineering to introduce multicomponent pathways into heterologous host for production of metabolites. However, this process sometimes may take weeks months due the lack standardized genetic tools. Here, we present method design and construction biological parts based on native genes regulatory elements Saccharomyces cerevisiae. We have developed highly efficient protocols (termed YeastFab Assembly) synthesize these as parts, which can be used assemble...
With the decreasing cost of next-generation sequencing, whole-genome sequence-based bacterial genome comparisons are expected to become a mainstream process in public health domain. Extended multilocus sequence typing (MLST) methods becoming increasingly popular for use comparing genetic relatedness epidemiological investigations. Several extended MLST schemes based on biological signatures have been reported. Among them, (wgMLST) has gradually one most widely used approaches strain typing....
The bacterium Escherichia coli remains the leading host for protein expression in large quantity purpose of crystallization or other biochemical studies. However, multicomponent complexes a challenge, and is often laborious time-consuming. Here we developed method named EcoExpress, which allows efficient construction plasmids to express individual with user-defined epitope-tag, followed by one-pot assembly single vector entire complex copurification. A versatile set vectors was designed...
Poly(dG)−poly(dC) DNA and G4-DNA are two types of synthetic molecules that regarded as promising candidates for molecular nanodevices. In this work, vibrating scanning polarization force microscopy (VSPFM) was employed to study the radial deformability these coadsorbed on a Ni2+-modified mica surface. The values compressive elastic modulus were found be similar (∼5−10 MPa) when external loading between ∼0.04 ∼0.12 nN. However, possessed higher stiffness than poly(dG)−poly(dC) (∼20−40 vs...
This paper presents a safety analysis method based on mass re-distribution experiment of the novel 4-Wheel Independent in-wheel-motor Drive and Steering Electrical Vehicle (4WIDIS EV), which has four independent wheel units with central engine, gearbox steering mechanism all removed, as each unit an in-wheel motor its own control system. A is used to coordinate 4 wheels translate driver's input so that there no mechanical link between these units. In such design, compare traditional vehicle,...
Dredging engineering projects are complex because they involve greater uncertainty from the natural environment, social needs, government policy and many stakeholders. Engineering companies submit tenders that draw on similar cases undertaken in recent years. However, weather, earthquakes, typhoons other disasters often change landforms. Therefore, evaluating duration of dredging with reference to only a few previous is inadequate, leading an unnecessarily long construction if scope project...
Extended multi-locus sequence typing (eMLST) methods have become popular in the field of genomic epidemiology. Before eMLST can be applied epidemiological investigations, selection a suitable scheme is critical. The core genome (cgMLST) has most approach for strain domain. In addition to typing, many public health researchers and clinical microbiologists wish investigate which genes cause genetic differences between compared strains. Therefore, tool that used extract canonical with an would...
Gene synthesis is gaining popularity as a versatile means of cloning genes. The prevailing methods are PCR-based and ligase-based gene synthesis. Both them not independent the sequence content target gene. In present study, new method-isothermal unidirectional elongation method (IUEM) proposed, which involves cooperation three enzymes, or partially by specially designed oligonucleotides that form hairpins. process isothermal serial in IUEM. Several factors affect were studied, two DNA...