A5025683597- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- CRISPR and Genetic Engineering
- Enzyme Catalysis and Immobilization
- Biocrusts and Microbial Ecology
- Tardigrade Biology and Ecology
- Animal Genetics and Reproduction
- Microbial Metabolites in Food Biotechnology
- Enzyme-mediated dye degradation
- Lichen and fungal ecology
- Biomedical and Engineering Education
- Mycotoxins in Agriculture and Food
- Fungal Biology and Applications
- Genetics, Bioinformatics, and Biomedical Research
- Peptidase Inhibition and Analysis
- Chemistry and Chemical Engineering
- Wildlife Ecology and Conservation
- Law, AI, and Intellectual Property
- Genomic variations and chromosomal abnormalities
- Sustainability and Climate Change Governance
- Lipid metabolism and biosynthesis
- Reproductive Physiology in Livestock
- Cancer Genomics and Diagnostics
Bioplatforms Australia
2019-2023
Macquarie University
1999-2023
ARC Centre of Excellence in Synthetic Biology
2020-2023
National Measurement Institute
2010
Biofoundries provide an integrated infrastructure to enable the rapid design, construction, and testing of genetically reprogrammed organisms for biotechnology applications research. Many biofoundries are being built a Global Biofoundry Alliance has recently been established coordinate activities worldwide.
Accurate estimation of total DNA concentration (mass concentration, e.g., ng/μL) that is traceable to the International System Units (SI) a crucial starting point for improving reproducible measurements in many applications involving nucleic acid testing and requires reference material which has been certified its concentration. In this study, concentrations six different lambda preparations were determined using measurement platforms: UV Absorbance at 260 nm (A260) with without prior sodium...
A PCR method suitable for the isolation of lipase genes directly from environmental DNA is described. The problems associated with low levels similarity between were overcome by extensive analysis conserved regions and careful primer design. Using this method, a gene (oli-lipase) was isolated DNA. This showed less than 20% other known lipases at amino acid level. study also revealed that distantly related members α/β hydrolase superfamily share similar motifs lipases, thus making these...
Synthetic chromosome engineering is a complex process due to the need identify and repair growth defects deal with combinatorial gene essentiality when rearranging chromosomes. To alleviate these issues, we have demonstrated novel approaches for repairing synthetic Saccharomyces cerevisiae genomes. We designed, constructed, restored wild-type fitness 753,096-bp version of S. XIV as part Yeast Genome project. In parallel use rational restore fitness, used adaptive laboratory evolution...
Abstract The reproductive biology of the ovoviviparous peripatus Euperipatoides rowelli was investigated from field collections and laboratory cultures. sexes have different demographics. frequency distribution individual weight is essentially L‐shaped in females, but closer to normality for males: thus must exhibit patterns growth and/or mortality. Males are generally much smaller rarer than females. primary sex ratio seems be 1:1 with equal investment sexes, while tertiary highly...
Onychophorans (peripatus or velvet worms) show extraordinarily high local endemism, and cryptic species are common. As part of a programme addressing issues endemicity at hierarchical spatial scales, we investigated reproduction in Euperipatoides rowelli (Onychophora: Peripatopsidae) using microsatellite analysis. This is ovoviviparous, females have up to 70 embryos their uteri simultaneously. Batches undeveloped well-developed may be present the female. Paired ovaries lead via common...
Abstract Filamentous fungi are lower eukaryotes increasingly used for expression of foreign proteins ranging from industrial enzymes originating other and bacteria to mammalian origin, such as antibodies growth factors. Their strengths include an excellent capacity protein secretion their eukaryotic processing machinery. Proteins secreted filamentous modified in the secretory pathway, with folding, proteolytic processing, addition glycans being main modifications. Unlike many systems,...
Cost-effective production of enzymes for industrial processes makes the appropriate selection host/vector expression system critical. We have tested two fungal systems bulk from thermophiles. The yeast Kluyveromyces lactis has been developed as a secretion host employing vectors based on 2u-like plasmid pKD1 drosophilarium. Our second involves filamentous fungus Trichoderma reesei. Signal and protein fusion constructed using strong cellobiohydrolase 1 (cbh1) promoter recombinant DNAs...
Immune-based fertility control is being considered as an effective long-term approach for controlling the pest brushtail possum (Trichosurus vulpecula) population in New Zealand. This relies heavily on immune response of each immunised possum. A strong and lasting majority individuals a essential. In this study, possums model macropod species, tammar wallaby (Macropus eugenii) were with either luteinising hormone or androgen receptor synthetic peptide coupled to carrier molecule KLH (keyhole...
Summary We have designed, constructed, and debugged a synthetic 753,096 bp version of Saccharomyces cerevisiae chromosome XIV as part the international Sc2.0 project. showed that certain loxPsym recombination sites can interfere with mitochondrial protein localization, deletion one intron ( NOG2 ) reduced fitness, reassigned stop codon lead to growth defect. In parallel these rational debugging modifications, we used Adaptive Laboratory Evolution generate general defect suppressor...
Abstract In Australia, synthetic biology has taken a gradual and rational passage of growth from collective discrete practitioners projects to capacity building initiatives. These initiatives fall under the National Innovation Science Agenda [2] have culminated into formation hubs scientific expertise education supported by leveraging enhancement existing infrastructure networks across country. The spotlight on science also motivated more whole‐of‐government response strengthening nation's...
The reproductive biology of the ovoviviparous peripatus Euperipatoides rowelli was investigated from field collections and laboratory cultures. sexes have different demographics. frequency distribution individual weight is essentially L-shaped in females, but closer to normality for males: thus must exhibit patterns growth and/or mortality. Males are generally much smaller rarer than females. primary sex ratio seems be 1:1 with equal investment sexes, while tertiary highly female-biased....