A5029884951- Fermentation and Sensory Analysis
- Microbial Metabolic Engineering and Bioproduction
- Horticultural and Viticultural Research
- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Biochemical and biochemical processes
- Enzyme Production and Characterization
- Mycobacterium research and diagnosis
- Enzyme Structure and Function
- Microbial Metabolites in Food Biotechnology
- Tuberculosis Research and Epidemiology
- Plant biochemistry and biosynthesis
- Wine Industry and Tourism
- Plant Pathogens and Fungal Diseases
- CRISPR and Genetic Engineering
- Carbohydrate Chemistry and Synthesis
- Phytochemicals and Antioxidant Activities
- Genomics and Phylogenetic Studies
- Tea Polyphenols and Effects
- Biochemical and Molecular Research
- Fungal Biology and Applications
- Cancer, Hypoxia, and Metabolism
- Innovative Microfluidic and Catalytic Techniques Innovation
- Bioeconomy and Sustainability Development
- Bacterial Genetics and Biotechnology
ARC Centre of Excellence in Synthetic Biology
2020-2025
Macquarie University
2019-2025
Brain (Germany)
2025
Hochschule Geisenheim University
2018-2024
Université de Montpellier
2016-2020
Centre National de la Recherche Scientifique
2016-2020
Institut de Recherche en Infectiologie de Montpellier
2018-2020
The University of Sydney
2018
Washington State University
2018
AgroParisTech
2018
Abstract Background The lignocellulosic enzymes of Trichoderma species have received particular attention with regard to biomass conversion biofuels, but the production cost these remains a significant hurdle for their commercial application. In this study, we quantitatively compared lignocellulolytic enzyme profile newly isolated asperellum S4F8 strain that reesei Rut C30, cultured on sugarcane bagasse (SCB) using solid-state fermentation (SSF). Results Comparison profiles and C30 showed...
Synthetic chromosome engineering is a complex process due to the need identify and repair growth defects deal with combinatorial gene essentiality when rearranging chromosomes. To alleviate these issues, we have demonstrated novel approaches for repairing synthetic Saccharomyces cerevisiae genomes. We designed, constructed, restored wild-type fitness 753,096-bp version of S. XIV as part Yeast Genome project. In parallel use rational restore fitness, used adaptive laboratory evolution...
In this study, the aroma-production profiles of seven different Hanseniaspora strains, namely H. guilliermondii, meyeri, nectarophila, occidentalis, opuntiae, osmophila and uvarum were determined in a simultaneous co-inoculation with wine yeast Saccharomyces cerevisiae Champagne Epernay Geisenheim (Uvaferm CEG). All co-inoculated fermentations showed dramatic increase ethyl acetate levels except two (H. occidentalis osmophila) that belong to so-called slow-evolving clade, which had no...
Abstract The Sc2.0 global consortium to design and construct a synthetic genome based on the Saccharomyces cerevisiae commenced in 2006, comprising 16 chromosomes new-to-nature tRNA neochromosome. In this paper we describe assembly debugging of 902,994-bp chromosome synXVI project. Application CRISPR D-BUGS protocol identified defective loci, which were modified improve sporulation recover wild-type like growth when grown glycerol as sole carbon source at 37˚C. LoxPsym sites inserted...
Yeast—especially Saccharomyces cerevisiae—have long been a preferred workhorse for the production of numerous recombinant proteins and other metabolites. S. cerevisiae is noteworthy aroma compound producer has also exploited to produce foreign bioflavour compounds. In past few years, important strides have made in unlocking key elements biochemical pathways involved many The expression these yeast often involves manipulation host strain direct flux towards certain precursors needed given...
Sucrolytic enzymes catalyse sucrose hydrolysis or the synthesis of fructooligosaccharides (FOSs), a prebiotic in human and animal nutrition. FOS capacity differs between sucrolytic enzymes. Amino-acid-sequence-based classification synthesizing would greatly facilitate silico identification novel catalysts, as large amounts sequence data lie untapped. The development bioinformatics tool to rapidly distinguish high-level FOSs predominantly hydrolysing from fungal genomic is presented. Sequence...
Apiculate yeasts belonging to the genus Hanseniaspora are commonly isolated from viticultural settings and often dominate initial stages of grape must fermentations. Although considered spoilage yeasts, they now increasingly becoming focus research, with several whole-genome sequencing studies published in recent years. However, tools for their molecular genetic manipulation still lacking. Here, we report development a tool modification uvarum. This was employed disruption HuATF1 gene, which...
It was recently shown that Mycobacterium tuberculosis produces cellulose which forms an integral part of its extracellular polymeric substances within a biofilm set-up. Using smegmatis as proxy model organism, we demonstrate M. biofilms treated with purified MSMEG_6752 releases the main degradation-product (cellobiose), detected by using ionic chromatography, suggesting encodes cellulase. Its overexpression in prevents spontaneous formation. Moreover, method reported here allowed detecting...
Recently, there has been a growing interest in the role of non-Saccharomyces yeast (NSY) as coculturing partner with Saccharomyces cerevisiae during grape must fermentation. We investigated three new strains, namely Nakazawaea ishiwadae, Candida railenensis and Debaryomyces hansenii, for their oenological potential mixed-culture micro-vinifications S. Vin13 using Muscaris must. None NSY strains impeded fermentation performance all experiments finished at same time. Coculturing N. ishiwadae...
In this study, the impact of apiculate yeast Hanseniaspora occidentalis as a co-partner with Saccharomyces cerevisiae was investigated in sequential-type mixed-culture fermentation Muscaris grape must. As other trials using strains, significant increase ethyl acetate observed, but most intriguing almost complete abolition malic acid (from 2.0 g/L to 0.1 g/L) wine. Compared pure S. inoculum, there also marked concentrations esters. Modulation some varietal elements, such rose oxide, observed....
Abstract Background β-Fructofuranosidases (or invertases) catalyse the commercially-important biotransformation of sucrose into short-chain fructooligosaccharides with wide-scale application as a prebiotic in functional foods and pharmaceutical industries. Results We identified β-fructofuranosidase gene ( CmINV ) from Ceratocystis moniliformis genome sequence using protein homology phylogenetic analysis. The predicted 615 amino acid protein, CmINV, grouped an existing clade within glycoside...
Traditional kombucha is a functional tea-based drink that has gained attention as low or non-alcoholic beverage. The fermentation conducted by community of different microorganisms, collectively called SCOBY (Symbiotic Culture Bacteria and Yeast) typically consists acetic acid bacteria fermenting yeast, in some cases lactic would convert the sugars into organic acids-mostly acid. In this study, effect including Pichia kluyveri starter culture was investigated. P. additions led to quicker...
Abstract: Yeast – especially Saccharomyces cerevisiae have long been a preferred workhorse for the production of numerous recombinant proteins and other metabolites. S. is noteworthy aroma compound producer, has also exploited to produce foreign bioflavour compounds. In past few years, important strides made in unlocking key elements biochemical pathways involved many The expression these yeast often involves manipulation host strain direct flux towards certain precursors needed...