A5103241725- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- CRISPR and Genetic Engineering
- Music History and Culture
- Viral Infectious Diseases and Gene Expression in Insects
- Art, Politics, and Modernism
- Transgenic Plants and Applications
- American Political and Social Dynamics
- Biofuel production and bioconversion
Macquarie University
2023-2024
ARC Centre of Excellence in Synthetic Biology
2023-2024
Synthetic chromosome engineering is a complex process due to the need identify and repair growth defects deal with combinatorial gene essentiality when rearranging chromosomes. To alleviate these issues, we have demonstrated novel approaches for repairing synthetic Saccharomyces cerevisiae genomes. We designed, constructed, restored wild-type fitness 753,096-bp version of S. XIV as part Yeast Genome project. In parallel use rational restore fitness, used adaptive laboratory evolution...
Abstract Temperature is an important control factor for biologics biomanufacturing in precision fermentation. Here, we explored a highly responsive low temperature-inducible genetic system (LowTempGAL) the model yeast Saccharomyces cerevisiae. Two temperature biosensors, heat-inducible degron and protein aggregation domain, were used to regulate GAL activator Gal4p, rendering leaky LowTempGAL systems. Boolean-type induction was achieved by implementing second-layer through...
The variability in phenotypic outcomes among biological replicates engineered microbial factories presents a captivating mystery. Establishing the association between and genetic drivers is important to solve this intricate puzzle. We applied previously developed auxin-inducible depletion of hexokinase 2 as metabolic engineering strategy for improved nerolidol production Saccharomyces cerevisiae, exhibit dichotomy either 3.5 or 2.5 g L–1 nerolidol. Harnessing Oxford Nanopore's long-read...