A5037817223- Algal biology and biofuel production
- Polyamine Metabolism and Applications
- Microbial Metabolic Engineering and Bioproduction
- Antioxidant Activity and Oxidative Stress
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Phytase and its Applications
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- Transgenic Plants and Applications
- Amino Acid Enzymes and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Viral Infectious Diseases and Gene Expression in Insects
- Chemical synthesis and alkaloids
- Carbohydrate Chemistry and Synthesis
- Psychedelics and Drug Studies
- Genomics and Phylogenetic Studies
- Pancreatic function and diabetes
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Glycosylation and Glycoproteins Research
- Plant Virus Research Studies
- Alkaloids: synthesis and pharmacology
- Microbial Metabolites in Food Biotechnology
- Iron Metabolism and Disorders
Queensland University of Technology
2021-2024
Cell Biotech (South Korea)
2023
Biocat
2023
The University of Queensland
2016-2018
Universidad de Guadalajara
2010
Phytases are widely used commercially as dietary supplements for swine and poultry to increase the digestibility of phytic acid. Enzyme development has focused on increasing thermostability withstand high temperatures during industrial steam pelleting. Increasing often reduces activity at gut there remains a demand improved phyases growing market.In this work, we present thermostable variant E. coli AppA phytase, ApV1, that contains an extra non-consecutive disulfide bond. Detailed...
Traditionally derived from fossil fuels, biological production of propionic acid has recently gained interest. Propionibacterium species produce as their main fermentation product. Production other organic acids reduces yield and productivity, pointing to by-products gene-knockout strategies a logical solution increase yield. However, removing by-product formation seen limited success due our inability genetically engineer the best producing strains (i.e. acidipropionici). To overcome this...
Abstract Background Pichia pastoris ( Komagataella phaffii ) is an important platform for heterologous protein production due to its growth high cell density and outstanding secretory capabilities. Recent developments in synthetic biology have extended the toolbox genetic engineering of P. improve strains. Yet, overloading folding secretion capacity by over-expression recombinant proteins still issue rational design strains critical achieve cost-effective industrial manufacture. Several...
Abstract Astaxanthin (AX) is a carotenoid pigment with antioxidant properties widely used as feed supplement. Wild‐type strains of Phaffia rhodozyma naturally produce low AX yields, but we increased yields 50‐fold in previous research using random mutagenesis P. CBS6938 and fermentation optimization. On that study, genome changes were linked phenotype, relevant metabolic not resolved. In this the wild‐type superior mutant grown chemically defined media instrumented fermenters. Differential...
Abstract Temperature is an important control factor for biologics biomanufacturing in precision fermentation. Here, we explored a highly responsive low temperature-inducible genetic system (LowTempGAL) the model yeast Saccharomyces cerevisiae. Two temperature biosensors, heat-inducible degron and protein aggregation domain, were used to regulate GAL activator Gal4p, rendering leaky LowTempGAL systems. Boolean-type induction was achieved by implementing second-layer through...
Propionic acid (PA) is used as a food preservative and increasingly, precursor for the synthesis of monomers. PA produced mainly through hydrocarboxylation ethylene, also known 'oxo-process'; however, Propionibacterium species are promising biological producers natively producing their main fermentation product. However, to be competitive, yield at least 0.6 g/g required. A new strain able reach required was obtained using genome shuffling. To gain insight into changes leading improved...
Propionibacterium acidipropionici produces propionic acid as its main fermentation product. Traditionally derived from fossil fuels, environmental and sustainable issues have revived the interest in producing using biological resources. Here, we present closed sequence of ATCC 55737, an efficient producer.
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Abstract Pichia pastoris ( Komagataella phaffii ) is a fast‐growing methylotrophic yeast with the ability to assimilate several carbon sources such as methanol, glucose, or glycerol. It has been shown have outstanding secretion capability variety of heterologous proteins. In previous studies, we engineered P. co‐express Escherichia coli AppA phytase and HAC1 transcriptional activator using bidirectional promoter. Phytase production was characterized in shake flasks did not reflect industrial...
Abstract Tandem gene repeats naturally occur as important genomic features and determine many traits in living organisms, like human diseases microbial productivities of target bioproducts. Here, we develop a bacterial type-II toxin-antitoxin-mediated method to manipulate integration tandem Saccharomyces cerevisiae further visualise the evolutionary trajectories repeats. We designed tri-vector system introduce toxin-antitoxin-driven amplification (ToxAmp) modules, accidentally re-visited...
In synthetic biology, microbial chassis including yeast
Astaxanthin (AX) is a carotenoid pigment with antioxidant properties. AX used widely in the animal feed industry as supplement. Wild-type strains of Phaffia rhodozyma naturally produce low yields, but we increased yields 50-fold previous research using random mutagenesis P. CBS6938 and fermentation optimisation. Genome sequencing linked phenotype genome changes production relevant metabolic were not resolved. In this study, wild-type superior mutant grown chemically defined media...
Pichia pastoris ( Komagataella phaffii ) is a fast-growing methylotrophic yeast with the ability to assimilate several carbon sources such as methanol, glucose, or glycerol. It has been shown have outstanding secretion capability variety of heterologous proteins. In previous studies, we engineered P. co-express E. coli AppA phytase and HAC1 transcriptional activator using bidirectional promoter. Phytase production was characterised in shake flasks did not reflect industrial conditions....