A5084621795- Microbial infections and disease research
- Pneumonia and Respiratory Infections
- Genomics and Phylogenetic Studies
- Yersinia bacterium, plague, ectoparasites research
- Microbial Community Ecology and Physiology
- Bacterial Genetics and Biotechnology
- Blood groups and transfusion
- Gut microbiota and health
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Bacillus and Francisella bacterial research
- Plant-based Medicinal Research
- Bacteriophages and microbial interactions
- Microbial bioremediation and biosurfactants
- Methane Hydrates and Related Phenomena
- Bee Products Chemical Analysis
- Pharmacological Effects of Natural Compounds
- Dermatology and Skin Diseases
- Virus-based gene therapy research
- Urological Disorders and Treatments
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- Chromosomal and Genetic Variations
- RNA modifications and cancer
Procter & Gamble (United States)
2011-2025
Minhang District Central Hospital
2018-2025
Fudan University
1997-2025
Tongren University
2024
Nanchang University
2024
Yangzhou University
2024
Jinan University
2024
Nanjing Normal University
2023
Lawrence Berkeley National Laboratory
2007-2019
Huazhong University of Science and Technology
2019
Autophagy is a lysosome-dependent cellular catabolic mechanism mediating the turnover of intracellular organelles and long-lived proteins. Reduction autophagy activity has been shown to lead accumulation misfolded proteins in neurons may be involved chronic neurodegenerative diseases such as Huntington's disease Alzheimer's disease. To explore identify small molecules that can activate it, we developed series high-throughput image-based screens for small-molecule regulators autophagy. This...
Fungi in the genus Malassezia are ubiquitous skin residents of humans and other warm-blooded animals. involved disorders including dandruff seborrheic dermatitis, which together affect >50% humans. Despite importance common diseases, remarkably little is known at molecular level. We describe genome, secretory proteome, expression selected genes globosa. Further, we report a comparative survey genome proteome restricta, close relative implicated similar disorders. Adaptation to environment...
ABSTRACT The bacterium Caulobacter crescentus and related stalk bacterial species are known for their distinctive ability to live in low-nutrient environments, a characteristic of most heavy metal-contaminated sites. is model organism studying cell cycle regulation with well-developed genetics. We have identified the pathways responding heavy-metal toxicity C. provide insights possible application environmental restoration. exposed cells four metals (chromium, cadmium, selenium, uranium)...
Urothelium synthesizes a group of integral membrane proteins called uroplakins, which form two-dimensional crystals (urothelial plaques) covering >90% the apical urothelial surface. We show that ablation mouse uroplakin III (UPIII) gene leads to overexpression, defective glycosylation, and abnormal targeting Ib, presumed partner UPIII. The UPIII-depleted urothelium features small plaques, becomes leaky, has enlarged ureteral orifices resulting in back flow urine, hydronephrosis,...
Although water, small nonelectrolytes, and gases are freely permeable through most biological membranes, apical membranes of certain barrier epithelia exhibit extremely low permeabilities to these substances. The role integral membrane proteins in this function has been unclear. To study problem, we have ablated the mouse gene encoding uroplakin III (UPIII), one major protein subunits urothelial measured membranes. Ablation UPIII greatly diminishes amounts uroplakins on (Hu P, Deng FM, Liang...
Attachment of Mycoplasma pneumoniae to host cell by means a specialized terminus initiates infection. Monoclonal antibodies surface protein (Pl) inhibit this process, and react with region the tip covered peplomer-like particles. Since against Pl are generated natural experimental infection immunization, substance may be an important determinant protective immunity.
Identification of the attachment factor on virulent Mycoplasma pneumoniae organisms which permits surface parasitism respiratory epithelium was attempted. Brief pretreatment M. monolayers with protease prevented mycoplasma ot sensitive host cells without reducing viability microorganisms. Gel electrophoretic analysis proteins before and after exposure intact mycoplasmas to revealed absence a major protein species (P1) in enzyme-treated preparations while other bands exception P2 were...
ABSTRACT Yersinia pestis , the causative agent of bubonic and pneumonic plagues, has undergone detailed study at molecular level. To further investigate genomic diversity among this group to help characterize lineages plague organism that have no sequenced members, we present here genomes two isolates “classical” antiqua biovar, strains Antiqua Nepal516. The Nepal516 are 4.7 Mb 4.5 encode 4,138 3,956 open reading frames, respectively. Though both belong one three classical biovars, they...
The apical surface of mouse urothelium is covered by two-dimensional crystals (plaques) uroplakin (UP) particles. To study function, we ablated the UPII gene. A comparison phenotypes UPII- and UPIII-deficient mice yielded new insights into mechanism plaque formation some fundamental features urothelial differentiation. Although UPIII knockout small plaques, abolished formation, indicating that both heterodimers (UPIa/II UPIb/III or IIIb) are required for assembly. Both knockouts had elevated...
ABSTRACT Oil reservoirs are major sites of methane production and carbon turnover, processes with significant impacts on energy resources global biogeochemical cycles. We applied a cultivation-independent genomic approach to define microbial community membership predict roles for specific organisms in transformations Alaska North Slope oil fields. Produced water samples were collected from six locations between 1,128 m (24 27°C) 2,743 (80 83°C) below the surface. Microbial complexity...
The goal of the Human Microbiome Project (HMP) is to generate a comprehensive catalog human-associated microorganisms including reference genomes representing most common species. Toward this goal, HMP has characterized microbial communities at 18 body habitats in cohort over 200 healthy volunteers using 16S rRNA gene (16S) sequencing and generated nearly 1,000 from microorganisms. To determine how well current genome collections capture diversity observed among microbiome guide isolation...
The attachment of radioisotope-labeled Mycoplasma pneumoniae to hamster tracheal rings in organ culture was examined by radioautography and liquid scintillation counting. Radioautographs individual exposed for 8 h (3H) thymidine-labeled virulent M. revealed a dense extracellular collection emulsion grains along the luminal surface epithelial cells. Similar exposure isotope-labeled avirulent resulted no accumulation grains. numbers attached mycoplasmas, as measured counting infected rings,...
High-quality and disease-resistant male sterile lines have great potential for applications in hybrid rice breeding. We introduced specific mutations into the TMS5, Pi21, Xa13 genes Pinzhan intermediate breeding material using CRISPR/Cas9 multiplex genome editing system. found that transgene-free homozygous triple tms5/pi21/xa13 mutants obtained T1 generation displayed characteristics of thermosensitive genic sterility (TGMS) with enhanced resistance to blast bacterial blight. Our study...
Understanding the changes in skin microbiome and their relationship to host factors during aging remains largely unknown. To better understand this phenomenon, we collected samples for metagenomic factor analyses from forearm, buttock, facial 158 Caucasian females aged 20‒24, 30‒34, 40‒44, 50‒54, 60‒64, 70‒74 years. Metagenomics analysis was performed using 16S ribosomal RNA gene sequencing, whereas sebocyte gland area, lipids, natural moisturizing factors, antimicrobial peptides...
ABSTRACT Background Immunoglobulin A nephropathy (IgAN) is a major cause of primary glomerulonephritis characterized by mesangial deposits galactose-deficient IgA1 (Gd-IgA1). Toll-like receptors (TLRs), particularly TLR4, are involved in the pathogenesis IgAN. The role gut microbiota on IgAN patients was recently investigated. However, whether microbial modifications Gd-IgA1 through TLR4 play remains unclear. Methods We recruited subjects into four groups, including 48 with untreated IgAN,...
Erythromycin is the drug of choice for treatment Mycoplasma pneumoniae infections due to its susceptibility low levels this antibiotic. After exposure susceptible strains erythromycin in vitro and vivo, mutants resistant other macrolides were isolated. Their phenotypes have been characterized, but genetic basis resistance has never determined. We isolated two (M129-ER1 M129-ER2) by growing M. M129 on agar containing different amounts erythromycin. In broth dilution tests both displayed high...
ABSTRACT The complete nucleotide sequence and gene organization of the three virulence plasmids from Yersinia pestis KIM5 were determined. Plasmid pPCP1 (9,610 bp) has a GC content 45.3% encodes two previously known factors, an associated protein, single copy IS 100 . pCD1 (70,504 44.8%. It is to encode number essential determinants, regulatory functions, multiprotein secretory system comprising low-calcium response stimulation that shared with other species pathogenic for humans ( Y....
We examined the extent of correlation between home air nicotine levels and urine cotinine/creatinine ratios (CCR) in 27 children who attended a research day care program where they were not exposed to environmental tobacco smoke (ETS) during daytime hours. Average concentrations determined by active sampling evening night hours on 2 consecutive days. Urine samples for cotinine creatinine determinations collected before, during, after two periods. In addition, four sequential weekly CCR...
Zinc pyrithione (ZPT) is an antimicrobial material with widespread use in antidandruff shampoos and antifouling paints. Despite decades of commercial use, there little understanding its mechanism action. We used a combination genome-wide approaches (yeast deletion mutants microarrays) traditional methods (gene constructs atomic emission) to characterize the activity ZPT against model yeast, Saccharomyces cerevisiae. acts through increase cellular copper levels that leads loss iron-sulfur...
Even though human sweat is odorless, bacterial growth and decomposition of specific odor precursors in it believed to give rise body humans. While mechanisms generation have been widely studied adults, little known for teenagers pre-pubescent children who distinct composition from immature apocrine sebaceous glands, but are arguably more susceptible the social psychological impact malodor. We integrated information whole microbiome analysis multiple skin sites (underarm, neck, head) time...
The availability of draft sequences for both the mouse and human genomes makes it possible, first time, to annotate whole mammalian using comparative methods. TWINSCAN is a gene-prediction system that combines methods single-genome predictors like GENSCAN with information derived from genome comparison, thereby improving accuracy. Because uses genomic sequence only, less biased toward highly and/or ubiquitously expressed genes than GENEWISE, GENOMESCAN, other based on evidence transcripts....