A5064415365- Neonatal Respiratory Health Research
- Anesthesia and Neurotoxicity Research
- Infant Development and Preterm Care
- Gut microbiota and health
- Birth, Development, and Health
- Dermatology and Skin Diseases
- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Infant Nutrition and Health
- Antibiotic Resistance in Bacteria
- Diet and metabolism studies
- Nail Diseases and Treatments
- Bacterial Identification and Susceptibility Testing
- Influenza Virus Research Studies
- Bee Products Chemical Analysis
- Dengue and Mosquito Control Research
- Dietary Effects on Health
- Retinal Diseases and Treatments
- Parkinson's Disease Mechanisms and Treatments
- Antibiotic Use and Resistance
- Pharmaceutical and Antibiotic Environmental Impacts
- Oral microbiology and periodontitis research
- Skin Protection and Aging
- Acne and Rosacea Treatments and Effects
- Ocular Diseases and Behçet’s Syndrome
Agency for Science, Technology and Research
2018-2025
Genome Institute of Singapore
2013-2025
Although disinfection is key to infection control, the colonization patterns and resistomes of hospital-environment microbes remain underexplored. We report first extensive genomic characterization microbiomes, pathogens antibiotic resistance cassettes in a tertiary-care hospital, from repeated sampling (up 1.5 years apart) 179 sites associated with 45 beds. Deep shotgun metagenomics unveiled distinct ecological niches genes characterized by biofilm-forming human-microbiome-influenced...
Sebaceous free fatty acids are metabolized by multiple skin microbes into bioactive lipid mediators termed oxylipins. This study investigated correlations between oxylipins and on the superficial of pre-pubescent children (N = 36) adults 100), including pre- 25) post-menopausal females 25). Lipidomics metagenomics revealed that Malassezia restricta positively correlated with oxylipin 9,10-DiHOME adult negatively its precursor, 9,10-EpOME, skin. Co-culturing keratinocytes demonstrated a link...
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth resolution. Existing Illumina protocols are, however, limited the fraction gene that is interrogated therefore limit resolution quality profiling. To address this, we present design a novel protocol for shotgun bacterial gene, optimized amplify more than 90% sequences Greengenes...
Androgenetic alopecia is the most common form of hair loss in males. It a multifactorial condition involving genetic predisposition and hormonal changes. The role microflora during remains to be understood. We therefore analyzed microbiome follicles from patients healthy. Hair were extracted occipital vertex region healthy volunteers further dissected into middle lower compartments. was then characterized by 16S rRNA sequencing. Distinct microbial population found compartment follicles....
Structural and molecular myelination deficits represent early pathological features of Huntington disease (HD). Recent evidence from germ-free (GF) animals suggests a role for microbiota-gut-brain bidirectional communication in the regulation myelination. In this study, we aimed to investigate impact microbiota on myelin plasticity oligodendroglial population dynamics mixed-sex BACHD mouse model HD. Ultrastructural analysis corpus callosum revealed alterations thickness GF compared...
Assess whether treatment with probiotics improve gastrointestinal symptoms in patients systemic sclerosis (SSc). In this double-blind randomized placebo-controlled parallel-group phase II trial, SSc subjects total score ≥ 0.1 on a validated SSc-specific tract (GIT) questionnaire were (1:1) to receive 60 days of high dose multi-strain (Vivomixx® 1800 billion units/day) or identical placebo, followed by an additional both groups. Between group differences GIT change assessed after (primary...
Abstract Background The compromised gut microbiome that results from C-section birth has been hypothesized as a risk factor for the development of non-communicable diseases (NCD). In double-blind randomized controlled study, 153 infants born by elective received an infant formula supplemented with either synbiotic, prebiotics, or unsupplemented until 4 months old. Vaginally were included reference group. Stool samples collected day 3 till week 22. Multi-omics deployed to investigate impact...
The human skin microbiome is increasingly recognized to influence health, immune function and disease susceptibility. However, large-scale, multi-site metagenomic studies in the general population remain scarce, limiting our understanding of full diversity variability microbial communities across different individuals body sites. Here, we present largest-to-date dataset (>3,550 shotgun metagenomes 18 sampling sites), generated using standardized cohort-wide protocols rigorous contaminant...
While the aetiology of age-related macular degeneration (AMD)-a major blinding disease-remains unknown, disease is strongly associated with variants in complement factor H (CFH) gene. CFH also confer susceptibility to invasive infection several bacterial colonizers nasopharyngeal mucosa. This shared locus implicates deregulation as a common mechanism, and suggests possibility that microbial interactions host may trigger AMD. In this study, we address by testing hypothesis AMD specific...
Background The influenza A virus is an RNA that responsible for seasonal epidemics worldwide with up to five million cases of severe illness and 500,000 deaths annually according the World Health Organization estimates. factors associated diseases are not well defined, but more disease often seen among persons aged >65 years, infants, pregnant women, individuals any age underlying health conditions. Methodology/Principal Findings Using gene expression microarrays, transcriptomic profiles...
Exposure to a diverse microbial environment during pregnancy and early postnatal period is important in determining predisposition towards allergy. However, the effect of environmental microbiota exposure preconception, life on development allergy child has not been investigated so far. In S-PRESTO (Singapore PREconception Study long Term maternal Outcomes) cohort, we collected house dust all three critical window periods analysed composition using 16S rRNA gene sequencing. At 6 18 months,...
Perturbance in the composition of human gut microbiota has been associated with metabolic disorders such as obesity, diabetes mellitus, and insulin resistance. The objectives this study are to examine effects ethnicity, central recorded dietary components on potentially influencing microbiome. We hypothesize that these factors have an influence microbiome.Subjects Chinese (n = 14), Malay 10), Indian 11) ancestry, a median age 39 years (range: 22-70 old), provided stool samples for microbiome...
Structural and molecular myelination deficits represent early pathological features of Huntington disease (HD). Recent evidence from germ-free (GF) animals suggests a role for microbiota-gut-brain bidirectional communication in the regulation myelination. In this study, we aimed to investigate impact microbiota on myelin plasticity oligodendroglial population dynamics mixed-sex BACHD mouse model HD. Ultrastructural analysis corpus callosum revealed alterations thickness GF compared...
ABSTRACT Structural and molecular myelination deficits represent early pathological features of Huntington disease (HD). Recent evidence from germ-free (GF) animals suggests a role for microbiota-gut-brain bidirectional communication in the regulation myelination. In this study, we aimed to investigate impact microbiota on myelin plasticity oligodendroglial population dynamics mixed-sex BACHD mouse model HD. Ultrastructural analysis corpus callosum revealed alterations thickness GF compared...
Abstract There is growing attention surrounding hospital acquired infections (HAIs) due to high associated healthcare costs, compounded by the scourge of widespread multi-antibiotic resistance. Although environment disinfection well acknowledged be key for infection control, an understanding colonization patterns and resistome profiles environment-dwelling microbes currently lacking. We report first extensive genomic characterization microbiomes (428), common HAI-associated (891)...
Abstract The distribution of microorganisms in built environments with high human traffic, such as food centres, can potentially have a significant impact on public health, particularly the context increasing worldwide incidence and fomite-related outbreaks. In several major Asian cities, centres are main venue for consumption yet we lack baseline understanding their environmental microbiomes. We conducted city-wide metagenomic surveillance food-centre microbiomes Singapore (16 n=240...
BackgroundNicaragua experienced a large Zika epidemic in 2016, with up to 50% of the population Managua infected. With domesticated Aedes aegypti mosquito as its vector, it is widely assumed that virus transmission occurs within household and/or via human mobility. We investigated these assumptions by using viral genomes trace spatially.MethodsWe analysed serum samples from 119 paediatric cases participating long-standing Paediatric Dengue Cohort Study Managua, which was expanded include...