A5061195209- Lipid metabolism and biosynthesis
- Dermatology and Skin Diseases
- Bee Products Chemical Analysis
- Endoplasmic Reticulum Stress and Disease
- Plant biochemistry and biosynthesis
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Skin Protection and Aging
- Nail Diseases and Treatments
- Plant-Microbe Interactions and Immunity
- Lysosomal Storage Disorders Research
- Autophagy in Disease and Therapy
- Photosynthetic Processes and Mechanisms
- Enzyme Catalysis and Immobilization
- Phytochemistry Medicinal Plant Applications
- Insect-Plant Interactions and Control
- Psoriasis: Treatment and Pathogenesis
- Research on Leishmaniasis Studies
- Essential Oils and Antimicrobial Activity
- Microbial Natural Products and Biosynthesis
- Trypanosoma species research and implications
- Acne and Rosacea Treatments and Effects
- Nuclear Structure and Function
- Hemoglobin structure and function
- Adipose Tissue and Metabolism
Firmenich (Switzerland)
2024-2025
Agency for Science, Technology and Research
2021-2025
National Skin Centre
2021
Institute of Plant and Microbial Biology, Academia Sinica
2019
UNSW Sydney
2016
University of Fribourg
2007-2012
University of Hawaii System
2012
ETH Zurich
2007
Berardinelli-Seip congenital lipodystrophy 2 (BSCL2) is caused by loss-of-function mutations in SEIPIN, a protein implicated both adipogenesis and lipid droplet expansion but whose molecular function remains obscure. Here, we identify physical functional interactions between SEIPIN microsomal isoforms of glycerol-3-phosphate acyltransferase (GPAT) multiple organisms. Compared to controls, GPAT activity was elevated SEIPIN-deficient cells tissues kinetic values were altered. Increased appears...
Sebaceous free fatty acids are metabolized by multiple skin microbes into bioactive lipid mediators termed oxylipins. This study investigated correlations between oxylipins and on the superficial of pre-pubescent children (N = 36) adults 100), including pre- 25) post-menopausal females 25). Lipidomics metagenomics revealed that Malassezia restricta positively correlated with oxylipin 9,10-DiHOME adult negatively its precursor, 9,10-EpOME, skin. Co-culturing keratinocytes demonstrated a link...
In yeast, phosphatidic acid, the biosynthetic precursor for all glycerophospholipids and triacylglycerols, is made de novo by 1-acyl-sn-glycerol-3-phosphate acyltransferases Ale1p Slc1p. belongs to membrane-bound O-acyltransferase (MBOAT) family, which contains many enzymes acylating lipids but also others that acylate secretory proteins residing in lumen of ER. A histidine present a very short loop between two predicted transmembrane domains only residue conserved throughout MBOAT gene...
Introduction: The human skin microbial composition is affected by age. Previous studies reported microbiome diversity shifts between elderly and significantly younger subjects. Some implied that menopausal status, which inherently linked to age, could be associated with changes in compositions. Nevertheless, the influence of status on profiles while minimizing impact aging-associated parameters still needs further clarification. Methods: We performed an observational study healthy Caucasian...
Seipin is known for its critical role in controlling lipid droplet (LD) assembly at the LD-forming subdomain of endoplasmic reticulum (ER). Here, we identified a new function seipin as negative regulator sphingolipid production. We show that yeast cells lacking displayed altered sensitivity to inhibitors, accumulated sphingoid precursors and intermediates, increased serine palmitoyltransferase (SPT) fatty acid (FA) elongase activities. associated with SPT FA elongase, interaction was reduced...
Disulfide bond formation in the endoplasmic reticulum is catalyzed by enzymes of protein disulfide-isomerase family that harbor one or more thioredoxin-like domains. We recently discovered transmembrane TMX3, a thiol-disulfide oxidoreductase family. Here, we show reticulum-luminal region TMX3 contains three domains, an N-terminal redox-active domain (named a) followed two enzymatically inactive domains (b and b'). Using recombinantly expressed constructs a, ab, abb', compared structural...
All glycerophospholipids are made from phosphatidic acid, which, according to the traditional view, is generated at cytosolic surface of ER. In yeast, acid synthesized de novo by two acyl-CoA-dependent acylation reactions. The first catalysed one homologous glycerol-3-phosphate acyltransferases Gpt2p/Gat1p and Sct1p/Gat2p, second 1-acyl-sn-glycerol-3-phosphate Slc1p Ale1p/Slc4p. To study biogenesis topology Gpt2p we observed location dual reporters inserted after various transmembrane...
Summary Glycosylphosphatidylinositol (GPI) lipids of Trypanosoma brucei undergo lipid remodelling, whereby longer fatty acids on the glycerol are replaced by myristate (C14:0). A similar process occurs GPI proteins Saccharomyces cerevisiae where Per1p first deacylates, Gup1p subsequently reacylates anchor lipid, thus replacing a shorter acid C26:0. Heterologous expression GUP1 homologue T. bruce i in gup1 Δ yeast cells partially normalizes phenotype and restores transfer labelled from...
Malassezia are common components of human skin, and as the dominant skin eukaryotic microbe, they take part in complex microbe-host interactions. Other phylogenetically related fungi (including within Ustilagomycotina) communicate with their plant host through bioactive oxygenated polyunsaturated fatty acids, generally known oxylipins, by regulating immune system to increase virulence. Oxylipins similar structure function eicosanoids, which modulate system. This study reports development a...
A key question that has remained unanswered is how pathogenic fungi switch from vegetative growth to infection-related morphogenesis during a disease cycle. Here, we identify fungal oxylipin analogous the phytohormone jasmonic acid (JA), as principal regulator of such developmental isotropic and pathogenicity in rice-blast fungus Magnaporthe oryzae. Using specific inhibitors mutant analyses, determined molecular function intrinsic jasmonates M. oryzae pathogenesis. Loss 12-Oxo-phytodienoic...
Abstract While research into gut-microbe interactions is common and advanced, with multiple defined impacts on human health, studies exploring the significance of skin-microbe remain underrepresented. Skin largest organ, has a vast surface area, inhabited by plethora microorganisms which metabolise sebaceous lipids. Sebaceous free fatty acids are metabolized bioactive lipid mediators immune-modulatory properties skin-resident microbes, including Malassezia . Intriguingly, many same also...
Abstract A key question that has remained unanswered is how pathogenic fungi switch from vegetative growth to infection-related morphogenesis during a disease cycle. Here, we identify fungal oxylipin analogous the well-known phytohormone jasmonic acid, as principal signal responsible for such developmental pathogenicity in rice-blast fungus Magnaporthe oryzae . We explored molecular function(s) of intrinsic acid differentiation M. via OPR1 , which encodes 12-Oxo-phytodienoic Acid Reductase...