A5005961131- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Gene expression and cancer classification
- BRCA gene mutations in cancer
- Cancer-related Molecular Pathways
- Molecular Biology Techniques and Applications
- Estrogen and related hormone effects
- Bioinformatics and Genomic Networks
- HER2/EGFR in Cancer Research
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- RNA Research and Splicing
- Breast Cancer Treatment Studies
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Genetics, Bioinformatics, and Biomedical Research
- Monoclonal and Polyclonal Antibodies Research
Jackson Laboratory
2016-2025
Genome Institute of Singapore
2007-2023
The JAX Cancer Center
2013-2023
Oncogenesis Stress Signaling
2001-2023
University of Connecticut
2015
Agency for Science, Technology and Research
2002-2012
College of the Atlantic
2012
National University of Singapore
2002-2011
Health Sciences Authority
2010
Dana-Farber Cancer Institute
2008
Comprehensive gene expression patterns generated from cDNA microarrays were correlated with detailed clinico-pathological characteristics and clinical outcome in an unselected group of 99 node-negative node-positive breast cancer patients. Gene found to be strongly associated estrogen receptor (ER) status moderately grade, but not menopausal status, nodal or tumor size. Hierarchical cluster analysis segregated the tumors into two main groups based on their ER which well basal luminal...
Perturbations of the p53 pathway are associated with more aggressive and therapeutically refractory tumors. However, molecular assessment status, by using sequence analysis immunohistochemistry, incomplete assessors functional effects. We posited that transcriptional fingerprint is a definitive downstream indicator function. Herein, we analyzed transcript profiles 251 p53-sequenced primary breast tumors identified clinically embedded 32-gene expression signature distinguishes p53-mutant...
The role of molecular markers in predicting the response to treatment breast cancer is poorly defined. Cancer and Leukemia Group B (CALGB) conducted a randomized adjuvant-chemotherapy trial (CALGB 8541) comparing three doses (high, moderate, low) cyclophosphamide, doxorubicin, fluorouracil 1572 women with node-positive cancer. This study 8869) was designed determine whether DNA index, S-phase fraction, c-erbB-2 expression, or p53 accumulation could be used as marker identify subgroup...
Polycomb-repressive complex 2 (PRC2)-mediated histone methylation plays an important role in aberrant cancer gene silencing and is a potential target for therapy. Here we show that S-adenosylhomocysteine hydrolase inhibitor 3-Deazaneplanocin A (DZNep) induces efficient apoptotic cell death cells but not normal cells. We found DZNep effectively depleted cellular levels of PRC2 components EZH2, SUZ12, EED inhibited associated H3 Lys 27 (but 9 methylation). By integrating RNA interference...
Adjuvant breast cancer therapy significantly improves survival, but overtreatment and undertreatment are major problems. Breast expression profiling has so far mainly been used to identify women with a poor prognosis as candidates for adjuvant without demonstrated value prediction.We obtained the gene profiles of 159 population-derived patients, hierarchical clustering signature associated impact therapies, defined distant metastasis or death within 5 years. Independent datasets 76 treated...
The human gut is known to be a reservoir of wide variety microbes, including viruses. Many RNA viruses are associated with gastroenteritis; however, the enteric viral community present in healthy humans has not been described. Here, we comparative metagenomic analysis found three fecal samples from two individuals. For this study, uncultured were concentrated by tangential flow filtration, and was extracted cloned into shotgun cDNA libraries for sequencing analysis. vast majority 36,769...
Abstract Histologic grading of breast cancer defines morphologic subtypes informative metastatic potential, although not without considerable interobserver disagreement and clinical heterogeneity particularly among the moderately differentiated grade 2 (G2) tumors. We posited that a gene expression signature capable discerning tumors 1 (G1) 3 (G3) histology might provide more objective measure with prognostic benefit for patients G2 disease. To this end, we studied profiles 347 primary...
Asia harbors substantial cultural and linguistic diversity, but the geographic structure of genetic variation across continent remains enigmatic. Here we report a large-scale survey autosomal from broad sample Asian human populations. Our results show that ancestry is strongly correlated with affiliations as well geography. Most populations relatedness within ethnic/linguistic groups, despite prevalent gene flow among More than 90% East (EA) haplotypes could be found in either Southeast...
Identification of lineage-specific innovations in genomic control elements is critical for understanding transcriptional regulatory networks and phenotypic heterogeneity. We analyzed, from an evolutionary perspective, the binding regions seven mammalian transcription factors (ESR1, TP53, MYC, RELA, POU5F1, SOX2, CTCF) identified on a genome-wide scale by different chromatin immunoprecipitation approaches found that only minority sites appear to be conserved at sequence level. Instead, we...
SummaryBackgroundThe cause of severe acute respiratory syndrome (SARS) has been identified as a new coronavirus. Whole genome sequence analysis various isolates might provide an indication potential strain differences this virus. Moreover, mutation will help to develop effective vaccines.MethodsWe sequenced the entire SARS viral cultured from index case (SIN2500) presenting in Singapore, three primary contacts (SIN2774, SIN2748, and SIN2677), one secondary contact (SIN2679). These sequences...
Using a chromatin immunoprecipitation-paired end diTag cloning and sequencing strategy, we mapped estrogen receptor alpha (ERalpha) binding sites in MCF-7 breast cancer cells. We identified 1,234 high confidence clusters of which 94% are projected to be bona fide ERalpha regions. Only 5% the located within 5 kb upstream transcriptional start adjacent genes, regions containing proximal promoters, whereas vast majority intronic or distal locations (>5 from 5' 3' ends transcript), suggesting...
Establishment of an in vivo small animal model human tumor and immune system interaction would enable preclinical investigations into the mechanisms underlying cancer immunotherapy. To this end, nonobese diabetic (NOD).Cg- PrkdcscidIL2rgtm1Wjl/Sz (null; NSG) mice were transplanted with (h)CD34+ hematopoietic progenitor stem cells, which leads to development systems [humanized NSG (HuNSG)]. HuNSG received leukocyte antigen partially matched implants from patient-derived xenografts [PDX;...
Zebrafish (Danio rerio) is a well-recognized model for the study of vertebrate developmental genetics, yet at same time little known about transcriptional events that underlie zebrafish embryogenesis. Here we have employed microarray analysis to temporal activity developmentally regulated genes during Transcriptome 12 different embryonic points covering five stages (maternal, blastula, gastrula, segmentation, and pharyngula) revealed highly dynamic profile. Hierarchical clustering,...