Christine A. Wells
A5072188738- Epigenetics and DNA Methylation
- Immune Response and Inflammation
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Gene expression and cancer classification
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Bioinformatics and Genomic Networks
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Mesenchymal stem cell research
- Cardiomyopathy and Myosin Studies
- Gene Regulatory Network Analysis
- TGF-β signaling in diseases
- interferon and immune responses
- Cell Image Analysis Techniques
- Medical Imaging Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- 3D Printing in Biomedical Research
- Medical and Biological Sciences
Stem Cells Australia
2018-2024
The University of Melbourne
2016-2024
Unisys (United States)
2024
Griffith University
2000-2023
RMIT University
2023
Walter and Eliza Hall Institute of Medical Research
2017-2021
University of Siena
2021
Azienda Ospedaliera Universitaria Senese
2021
University of California, Los Angeles
2021
The University of Queensland
2005-2018
This study describes comprehensive polling of transcription start and termination sites analysis previously unidentified full-length complementary DNAs derived from the mouse genome. We identify 5' 3' boundaries 181,047 transcripts with extensive variation in arising alternative promoter usage, splicing, polyadenylation. There are 16,247 new protein-coding transcripts, including 5154 encoding proteins. Genomic mapping transcriptome reveals transcriptional forests, overlapping on both...
Antisense transcription (transcription from the opposite strand to a protein-coding or sense strand) has been ascribed roles in gene regulation involving degradation of corresponding transcripts (RNA interference), as well silencing at chromatin level. Global transcriptome analysis provides evidence that large proportion genome can produce both strands, and antisense commonly link neighboring "genes" complex loci into chains linked transcriptional units. Expression profiling reveals frequent...
Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There an international collaborative effort to identify all full-length mRNA transcripts from mouse, and ensure that each represented in physical collection clones. Here we report manual annotation 60,770 complementary DNA sequences. These are clustered 33,409 'transcriptional units', contributing 90.1% newly established transcriptome database. Of these transcriptional units, 4,258 new...
Abstract The mycobacterial cord factor trehalose-6,6-dimycolate (TDM) and its synthetic analog trehalose-6,6-dibehenate (TDB) are potent adjuvants for Th1/Th17 vaccination that activate Syk-Card9 signaling in APCs. In this study, we have further investigated the molecular mechanism of innate immune activation by TDM TDB. Syk-coupling adapter protein FcRγ was essential macrophage Th17 adjuvanticity. FcRγ-associated C-type lectin receptor Mincle expressed macrophages upregulated Recombinant...
The mononuclear phagocyte system (MPS) was defined as a family of cells comprising bone marrow progenitors, blood monocytes, and tissue macrophages. In this review, we briefly consider markers for lineage in the mouse, especially F4/80 surface antigen receptor macrophage colony-stimulating factor. concept MPS is challenged by evidence that there separate embryonic lineage, blurring boundaries between macrophages other types arising from phenotypic plasticity transdifferentiation, local...
Abstract The recognition of carbohydrate moieties by cells the innate immune system is emerging as an essential element in antifungal immunity, but despite number and diversity lectins expressed cells, few receptors have been characterized. Mincle, a C-type lectin, predominantly on macrophages, here shown to play role macrophage responses yeast Candida albicans. After exposure vitro, Mincle localized phagocytic cup, it was not for phagocytosis. In absence production TNF-α macrophages...
Abstract —Vascular endothelial growth factor-B (VEGF-B) is closely related to VEGF-A, an effector of blood vessel during development and disease a strong candidate for angiogenic therapies. To further study the in vivo function VEGF-B, we have generated Vegfb knockout mice ( −/− ). Unlike Vegfa mice, which die embryogenesis, are healthy fertile. Despite appearing overtly normal, hearts reduced size display vascular dysfunction after coronary occlusion impaired recovery from experimentally...
Mammalian genomes harbor a larger than expected number of complex loci, in which multiple genes are coupled by shared transcribed regions antisense orientation and/or bidirectional core promoters. To determine the incidence, functional significance, and evolutionary context mammalian we identified characterized 5,248 cis–antisense pairs, 1,638 promoters, 1,153 chains bidirectionally promoted pairs from 36,606 mouse transcriptional units (TUs), along with 6,141 2,113 1,480 42,887 human TUs....
Imprinted genes tend to occur in clusters. We have identified a cluster distal mouse chromosome (Chr) 2, known from early genetic studies contain both maternally and paternally imprinted, but unspecified, genes. Subsequently, one was as Gnas , which encodes G protein α subunit, there is clinical biochemical evidence that the human homologue GNAS1 mutated patients with Albright hereditary osteodystrophy, also imprinted. used representational difference analysis, based on parent-of-origin...
SUMMARY There is a pressing need for patient-derived cell models of brain diseases that are relevant and robust enough to produce the large quantities cells required molecular functional analyses. We describe here new model based on from human olfactory mucosa, organ smell, which regenerates throughout life neural stem cells. Olfactory mucosa biopsies were obtained healthy controls patients with either schizophrenia, neurodevelopmental psychiatric disorder, or Parkinson’s disease,...
The international FANTOM consortium aims to produce a comprehensive picture of the mammalian transcriptome, based upon an extensive cDNA collection and functional annotation full-length enriched cDNAs. previous dataset, FANTOM2, comprised 60,770 Functional revealed that this dataset contained only about half estimated number mouse protein-coding genes, indicating cDNAs still remained be collected identified. To pursue complete gene catalog covers all predicted cloning sequencing has been...
Finding and characterizing mRNAs, their transcription start sites (TSS), associated promoters is a major focus in post-genome biology. Mammalian cells have at least 5–10 magnitudes more TSS than previously believed, deeper sequencing necessary to detect all active given tissue. Here, we present new method for high-throughput of 5′ cDNA tags—DeepCAGE: merging the Cap Analysis Gene Expression with ultra-high-throughput sequence technology. We apply DeepCAGE characterize 1.4 million sequenced...
Gene expression analysis has become a ubiquitous tool for studying wide range of human diseases. In typical we compare distinct phenotypic groups and attempt to identify genes that are, on average, significantly different between them. Here describe an innovative approach the gene data, one identifies differences in variance as informative metric group phenotype. We find with profiles are not randomly distributed across cell signaling networks. Genes low-expression variance, or higher...
Background Without appropriate cellular models the etiology of idiopathic Parkinson's disease remains unknown. We recently reported a novel patient-derived model generated from biopsies olfactory mucosa (termed neurosphere-derived (hONS) cells) which express functional and genetic differences in disease-specific manner. Transcriptomic analysis Patient Control hONS cells identified NRF2 transcription factor signalling pathway as most differentially expressed disease. Results tested robustness...
Abstract Reprogramming of somatic cells to induced pluripotent stem involves a dynamic rearrangement the epigenetic landscape. To characterize this epigenomic roadmap, we have performed MethylC-seq, ChIP-seq (H3K4/K27/K36me3) and RNA-Seq on samples taken at several time points during murine secondary reprogramming as part Project Grandiose. We find that DNA methylation gain occurs gradually, while loss is achieved only ESC-like state. Binding sites activated factors exhibit focal...