A5069875485- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA Research and Splicing
- Neurogenetic and Muscular Disorders Research
- Nerve injury and regeneration
- Pain Mechanisms and Treatments
- Mobile Health and mHealth Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Digital Mental Health Interventions
- Neurogenesis and neuroplasticity mechanisms
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Peroxisome Proliferator-Activated Receptors
- Spinal Cord Injury Research
- Signaling Pathways in Disease
- Technology Use by Older Adults
- Attention Deficit Hyperactivity Disorder
- Extracellular vesicles in disease
- Augmented Reality Applications
- Neurofibromatosis and Schwannoma Cases
- Animal testing and alternatives
- Protein Kinase Regulation and GTPase Signaling
- Neurological disorders and treatments
Roche (Switzerland)
2023
Nationwide Children's Hospital
2016-2019
Neurological Surgery
2013-2017
University of Miami
2010-2017
Scuola Internazionale Superiore di Studi Avanzati
2008-2010
International Centre for Genetic Engineering and Biotechnology
2006
Axon regeneration in the central nervous system normally fails, part because of a developmental decline intrinsic ability CNS projection neurons to extend axons. Members KLF family transcription factors regulate regenerative potential developing neurons. Expression one member, KLF7, is down-regulated developmentally, and overexpression KLF7 cortical vitro promotes axonal growth. To circumvent difficulties achieving high neuronal expression exogenous we created chimera with VP16...
Finding and characterizing mRNAs, their transcription start sites (TSS), associated promoters is a major focus in post-genome biology. Mammalian cells have at least 5–10 magnitudes more TSS than previously believed, deeper sequencing necessary to detect all active given tissue. Here, we present new method for high-throughput of 5′ cDNA tags—DeepCAGE: merging the Cap Analysis Gene Expression with ultra-high-throughput sequence technology. We apply DeepCAGE characterize 1.4 million sequenced...
Tumor necrosis factor (TNF) is associated with the pathophysiology of various neurological disorders, including multiple sclerosis. It exists as a transmembrane form tmTNF, signaling via TNF receptor 2 (TNFR2) and TNFR1, soluble form, solTNF, TNFR1. Multiple sclerosis detrimental effects solTNF acting through while tmTNF promotes repair remyelination. Here we demonstrate that oligodendroglial TNFR2 key mediator tmTNF-dependent protection in experimental autoimmune encephalomyelitis (EAE)....
Although infiltrating macrophages influence many pathological processes after spinal cord injury (SCI), the intrinsic molecular mechanisms that regulate their function are poorly understood. A major hurdle has been dissecting macrophage-specific functions from those in other cell types as well understanding how change over time. Therefore, we used RiboTag method to obtain mRNA directly injured mice and performed RNA sequencing investigate transcriptional profile. Our data show at 7 d SCI,...
CLN6-Batten disease, a form of neuronal ceroid lipofuscinosis is rare lysosomal storage disorder presenting with gradual declines in motor, visual, and cognitive abilities early death by 12-15 years age. We developed self-complementary adeno-associated virus serotype 9 (scAAV9) vector expressing the human CLN6 gene under control chicken β-actin (CB) hybrid promoter. Intrathecal delivery scAAV9.CB.hCLN6 into cerebrospinal fluid (CSF) lumbar spinal cord 4-year-old non-human primates was safe,...
We have studied the splicing regulation of NF1 exons 36 and 37. show that they not only require an intact exonic Splicing Enhancer (ESE) within exon 37, but also need genomic region stretching from 31 to 38. Any nucleotide change in two 37 third codon positions disrupts ESE. The extent skipping due a mutated ESE depends on context. This is unique example what may be more general phenomena involved tuning pre‐mRNA processing gene expression modulation chromosomal setting.
To fully understand cell type identity and function in the nervous system there is a need to neuronal gene expression at level of isoform diversity. Here we applied Next Generation Sequencing transcriptome (RNA-Seq) purified sensory neurons cerebellar granular (CGNs) grown on an axonal growth permissive substrate. The goal analysis was uncover specific isoforms as prelude understanding patterns underlying their intrinsic abilities. Global were comparable those found for other types, that...
Stress and glucocorticoid (GC) release are common behavioral hormonal responses to injury or disease. In the brain, stress/GCs can alter neuron structure function leading cognitive impairment. GCs also exacerbate pain, but whether a corresponding change occurs in structural plasticity of sensory neurons is unknown. Here, we show that female mice (Mus musculus) basal GC receptor (Nr3c1, known as GR) expression dorsal root ganglion (DRG) 15-fold higher than canonical stress-responsive brain...
CGG repeat expansions in the Fragile X mental retardation 1 (FMR1) gene are responsible for a family of associated disorders characterized by either intellectual disability and autism Syndrome (FXS), or adult-onset neurodegeneration X-associated Tremor/Ataxia Syndrome. However, FMR1 locus is complex encodes several long non-coding RNAs, whose expression altered expansion mutations. The role these lncRNAs thus far unknown; therefore we investigated functionality FMR4, which previously...
Abstract We report gene profiling data on genomic processes underlying the progression towards recurrent seizures after injection of kainic acid (KA) into mouse hippocampus. Focal enabled us to separate effects proepileptic stimuli initiated by KA injection. Both injected and contralateral hippocampus participated in status epilepticus. However, neuronal death induced treatment was restricted hippocampus, although there some axonal degeneration. profiled expression changes dorsal ventral...
The R7 family of regulators G protein signaling (RGS) is involved in many functions the nervous system. This includes RGS6, RGS7, RGS9, and RGS11 gene products defined by presence characteristic first found Disheveled, Egl-10, Pleckstrin (DEP), DEP helical extension (DHEX), Gγ-like, RGS domains. Herein, we examined subcellular localization most broadly expressed member. Our immunofluorescence studies retinal dorsal root ganglion neurons showed that RGS7 concentrated at plasma membrane cell...
Animal models of sciatic nerve injury are commonly used to study neuropathic pain as well axon regeneration. Administration post-surgical analgesics is an important consideration for animal welfare, but the actions analgesic must not interfere with scientific goals experiment. In this study, we show that treatment either buprenorphine or acetaminophen following a bilateral crush surgery does alter expression in dorsal root ganglion (DRG) sensory neurons panel genes associated wound healing....
<sec> <title>BACKGROUND</title> Digital therapeutics (DTx) are software-based products that prevent, manage, or treat a medical condition and delivered through smartphone app, web application, wearable device. Clinical trials assessing DTx pose challenges, foremost among which is designing appropriate <i>digital shams</i> (or placebos</i>), should ideally mimic (in terms of design, components, duration treatment) while omitting the active principle component. </sec>...