A5018862562- Advancements in Transdermal Drug Delivery
- Lipid Membrane Structure and Behavior
- Advanced Drug Delivery Systems
- Surfactants and Colloidal Systems
- Essential Oils and Antimicrobial Activity
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Parkinson's Disease Mechanisms and Treatments
- Pharmacological Effects of Natural Compounds
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Dermatology and Skin Diseases
- Neurogenetic and Muscular Disorders Research
- Enhanced Recovery After Surgery
- Cardiac, Anesthesia and Surgical Outcomes
- Toxin Mechanisms and Immunotoxins
- Bee Products Chemical Analysis
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Ultrasound and Hyperthermia Applications
- Signaling Pathways in Disease
- Colorectal Cancer Surgical Treatments
- Ultrasound and Cavitation Phenomena
- Connexins and lens biology
- Ocular Surface and Contact Lens
Sapienza University of Rome
2012-2024
Scientific Centre of Monaco
2024
Toscana Life Sciences
2024
Fondazione Santa Lucia
2024
PowerCell (Sweden)
2024
Nationwide Children's Hospital
2016-2023
University of Turin
2021
University of Rome Tor Vergata
2021
Italian Institute of Technology
2014-2020
Multidisciplinary Digital Publishing Institute (Switzerland)
2019
To examine the pathogenic role of α-synuclein (αS) in Parkinson's Disease, we have generated induced Pluripotent Stem Cell lines from early onset Disease patients with SNCA A53T and Triplication mutations, this study differentiated them to PSC-macrophages (pMac), which recapitulate many features their brain-resident cousins, microglia. We show that pMac, but not significantly increased intracellular αS versus controls release more medium. reduced phagocytosis capability can be phenocopied by...
The aim of this in vitro study is to prepare and characterize drug free pentamidine loaded chitosan glutamate coated niosomes for intranasal delivery reach the brain through delivery. Mucoadhesive properties stability testing various environments were evaluated examine potential these formulations be effective vehicles brain. Samples prepared using thin film hydration method. Changes size ζ-potential uncoated with without loading conditions assessed by dynamic light scattering (DLS), while...
Cerebral Dopamine Neurotrophic Factor (CDNF) and Mesencephalic Astrocyte-derived factor (MANF) are members of a recently discovered family neurotrophic factors (NTFs). Here, we used intranigral or intrastriatal lentiviral vector-mediated expression to evaluate their efficacy at protecting dopaminergic function in the 6-OHDA model Parkinson's disease (PD). In contrast well-studied Glial-Derived (GDNF), no beneficial effects were demonstrated by striatal overexpression either protein....
Magnetic force microscopy (MFM) is an atomic (AFM) based technique in which AFM tip with a magnetic coating used to probe local fields the typical spatial resolution, thus allowing one acquire images reflecting properties of samples at nanoscale. Being well established tool for characterization recording media, superconductors and nanomaterials, MFM finding constantly increasing application study materials systems biological biomedical interest. After reviewing these latter applications,...
Non-neuronal cell types such as astrocytes can contribute to Parkinson's disease (PD) pathology. The G2019S mutation in leucine-rich repeat kinase 2 (LRRK2) is one of the most common known causes familial PD. To characterize its effect on astrocytes, we developed a protocol produce midbrain-patterned from human induced pluripotent stem cells (iPSCs) derived PD LRRK2 patients and healthy controls. RNA sequencing analysis revealed downregulation genes involved extracellular matrix cases. In...
The aim of the present work is to develop nanoemulsions (NEs), nanosized emulsions, manufactured for improving delivery active pharmaceutical ingredients. In particular, composed Neem seed oil, contain rich bioactive components, and Tween 20 as nonionic surfactant were prepared. A mean droplet size ranging from 10 100 nm was obtained by modulating oil/surfactant ratio. Physicochemical characterisation carried out evaluating size, ζ-potential, microviscosity, polarity turbidity external shell...
Chemical fingerprints of four different Satureja montana L. essential oils (SEOs) were assayed by an untargeted metabolomics approach based on Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) coupled with either electrospray ionization or atmospheric pressure chemical sources. Analysis and relative quantification the non-polar volatile fraction conducted gas chromatography (GC) to MS. FT-ICR MS confirmed significant differences in polar metabolite composition, while...
ABSTRACT – Purpose. Aim of this research was to prepare and study drug release from a new formulation consisting non ionic surfactant vesicular structures, niosomes (NSVs), loaded with model molecules calcein (CALC), nile red (NR), ibuprofen (IBU) or caffeine (CAFF), embedded in hydrogel matrix. Methods. The system locust bean gum/xanthan (1:1), prepared at 60 °C, used entrap the vesicles (Tween 20/cholesterol 1:1), guest profiles were detected 32 °C. systems characterized by means scanning...
The use of nanocarriers, which respond to different stimuli controlling their physicochemical properties and biological responsivness, shows a growing interest in pharmaceutical science. are activated by targeting tissues compartments, e.g., pH modification, temperature, redox condition, enzymatic activity, or can be physically applied, magnetic field ultrasound. modification represents the easiest method passive targeting, is actually used accumulate nanocarriers cells tissues. aim this...
CLN6-Batten disease, a form of neuronal ceroid lipofuscinosis is rare lysosomal storage disorder presenting with gradual declines in motor, visual, and cognitive abilities early death by 12-15 years age. We developed self-complementary adeno-associated virus serotype 9 (scAAV9) vector expressing the human CLN6 gene under control chicken β-actin (CB) hybrid promoter. Intrathecal delivery scAAV9.CB.hCLN6 into cerebrospinal fluid (CSF) lumbar spinal cord 4-year-old non-human primates was safe,...
Brain infections as meningitis and encephalitis are attracting a great interest. Challenges in the treatment of these diseases mainly represented by blood brain barrier (BBB) that impairs efficient delivery even very potent drugs to reach brain. The nose administration route, is non-invasive alternative for quick onset action, enables transport numerous medicinal agents straight thus workarounding BBB through highly vascularized olfactory region. In this report, Thymus vulgaris Syzygium...
Several phytochemical-containing herbal extracts are increasingly marketed as health-promoting products. In particular, chamomile (Matricaria recutita L.) is well known for its anti-inflammatory, analgesic, and antitumor properties. Here, we evaluated differences in chemical composition among six commercially available products their potential impact on biological activity human immortalized colonocytes. Our investigation encompassed: (i) preparation of dry yield evaluation; (ii) qualitative...
Background Gap junction communication has been shown in glial and neuronal cells it is thought they mediate inter- intra-cellular communication. Connexin 36 (Cx36) expressed extensively the developing brain, with levels peaking at P14 after which its fall expression becomes entirely neuronal. These other data have led to hypothesis that Cx36 may direct coupling neurogenesis during development. Methodology/Principal Findings To investigate function we used a neurosphere model of cell...
Background: Liquorice extracts demonstrate therapeutic efficacy in treating dermatitis, eczema, and psoriasis when compared with corticosteroids. In this work, nonionic surfactant vesicles (niosomes, NSVs) containing polysorbate 20 (Tween 20), cholesterol, cholesteryl hemisuccinate at different molar concentrations were used to prepare monoammonium glycyrrhizinate (AG)-loaded NSVs. The anti-inflammatory properties of AG-loaded NSVs investigated murine models. Methods: physicochemical the...
pH-sensitive nonionic surfactant vesicles (niosomes) by polysorbate-20 (Tween-20) or derivatized glycine (added as pH sensitive agent), were developed to deliver Ibuprofen (IBU) and Lidocaine (LID). For the physical-chemical characterization of (mean size, size distribution, zeta potential, vesicle morphology, bilayer properties stability) dynamic light scattering (DLS), small angle X-ray fluorescence studies performed. Potential cytotoxicity was evaluated on immortalized human keratinocyte...
Silver nanoparticles (AgNPs) are widely used as antibacterial agents and anticancer drugs, but often their low stability limits mass production broad applications. The use of niosomes a carrier to protect envelop AgNPs gives new perspective solve these problems. In this study, were functionalized with sodium 3-mercapto-1-propanesulfonate (3MPS) induce hydrophilic behavior, improving loading in Tween 20 Span (NioTw20 NioSp20, respectively). Entrapment efficiency was evaluated by UV analyses...