A5019089059- Legionella and Acanthamoeba research
- Polyomavirus and related diseases
- Bacterial biofilms and quorum sensing
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Essential Oils and Antimicrobial Activity
- Antifungal resistance and susceptibility
- Clostridium difficile and Clostridium perfringens research
- Parasitic Infections and Diagnostics
- Microscopic Colitis
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Toxin Mechanisms and Immunotoxins
- Urinary Tract Infections Management
- Lipid Membrane Structure and Behavior
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Vibrio bacteria research studies
- Bacterial Identification and Susceptibility Testing
- Nosocomial Infections in ICU
- Crystallization and Solubility Studies
- Listeria monocytogenes in Food Safety
- Amoebic Infections and Treatments
- Medicinal Plants and Neuroprotection
Sapienza University of Rome
1999-2020
Policlinico Umberto I
2014
Czech Academy of Sciences, Institute of Microbiology
1999
Istituto Superiore di Sanità
1990-1999
University of Genoa
1999
Istituto Pasteur
1992-1994
Institut Pasteur
1986-1987
Summary Goose erythrocyte membranes were isolated and tested for their ability to compete with red cell receptors vesicular stomatitis virus (VSV) attachment fusion at acidic pH. Crude membranes, solubilized Triton X-100, Tween 80 octyl-β-d- glucopyranoside, showed a dose-dependent inhibitory effect on binding haemolysis. The chemical nature of the active molecules was investigated by enzyme digestion separation purified components. Only lipid moiety, specifically phospholipid glycolipid,...
Chemical fingerprints of four different Satureja montana L. essential oils (SEOs) were assayed by an untargeted metabolomics approach based on Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) coupled with either electrospray ionization or atmospheric pressure chemical sources. Analysis and relative quantification the non-polar volatile fraction conducted gas chromatography (GC) to MS. FT-ICR MS confirmed significant differences in polar metabolite composition, while...
Summary The role of gangliosides in rabies virus infection chick embryo-related (CER) cells was investigated. Cultured were pretreated with neuraminidase to render the transiently non-susceptible viral infection. Incubation these desialylated allowed them incorporate exogenous and they recovered their susceptibility Infection CER monitored by specific fluorescence 24 h after inoculation. use individual purified or mixtures two restore cellular showed that GT1b GQ1b most effective....
ABSTRACT We determined the incidence, risk factors, and outcomes of bloodstream infections (BSI) subsequent to Clostridium difficile infection (CDI). performed a retrospective study all patients with definite diagnosis CDI admitted from January 2014 December in two large hospitals Rome. Two groups were analyzed: those BSI (CDI/BSI + ) no evidence primary − ). Data about clinical features, microbiology, treatments, mortality obtained. Overall, 393 cases included final analysis: 72 developed...
ABSTRACT Five sporadic cases of nosocomial Legionnaires’ disease were documented from 1989 to 1997 in a hospital northern Italy. Two them, which occurred 75-year-old man suffering ischemic cardiopathy and an 8-year-old girl acute leukemia, had fatal outcomes. Legionella pneumophila serogroup 6 was isolated both patients hot-water samples taken at different sites the hospital. These facts led us consider possibility that single clone L. persisted environment for 8 years caused infections....
Fluoro-substituted flavones and 2-styrykhromones, related to natural synthetic flavonoids previously described, were prepared, characterized tested for anti-rhinovirus activity. Structural elucidation of the new compounds was performed by IR, NMR spectra X-ray crystal structure analysis 6-fluoro-3-hydroxy-2-styrylchromone. The antiviral potency evaluated a plaque reduction assay in HeLa cell cultures infected with rhinoviruses 1B 14, selected as representative serotypes viral groups B A...
The occurrence of legionnaires' disease has been described previously in passengers cruise ships, but determination the source rare. A 67-year-old, male cigarette smoker with heart contracted during a September 1995 and died 9 days after disembarking. Legionella pneumophila serogroup 1 was isolated from patient's sputum ship's water supply. Samples air-conditioning system were negative. L. isolates supply matched isolate, by both monoclonal antibody subtyping genomic fingerprinting. None 116...
Bloodstream infection (BSI) due to extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae are a major cause of in-hospital mortality. The effect on survival empirical and targeted antibiotic therapy in these patients remains controversial.A prospective cohort study was conducted analyzing data from 94 (age 59 ± 21 years) with BSI ESBL strains (Sixty-one E. coli, 26 K. pneumoniae, 4 Proteus spp 3 Enterobacter spp).Risk factors associated 21-day mortality at univariate analysis...
SUMMARY The effect of gangliosides extracted from human group O Rh+ erythrocytes on haemagglutination by BK virus was investigated. Experiments were performed both ganglioside mixtures and isolated fractions separated column chromatography characterized thin-layer chromatography. These results compared with those obtained standard preparations gangliosides, the inhibiting activity shown to be confined mainly a R Flower than GM1. It also observed that insertion in liposomes increased...
Abstract Urinary tract infections (UTIs) are among the most common bacterial in humans. Although a number of bacteria can cause UTIs, cases due to infection by uropathogenic Escherichia coli (UPEC). UPEC genetically heterogeneous group that exhibit several virulence factors associated with colonization and persistence urinary tract. Caenorhabditis elegans is tiny, free‐living nematode found worldwide. Because many biological pathways conserved C. humans, has been increasingly used as model...
Modifications of intestinal glycoreceptors expression, in particular CEACAM6, typically found ileal Crohn's disease (CD), favor, among the commensal species microbiota, enrichment Escherichia coli. Removal protein glycosidic residues by neuraminidase, a sialidase typical influenza virus, increases adhesion ability coli to Caco-2 cells. In this study we investigated whether virus infection human epithelial cells could influence adhesiveness different strains isolated from CD patients altering...
The growth inhibiting activity of transferrins, citrate, 2-2' dipyridyl and desferrioxamine methanesulphonate towards Legionella spp. their serogroups was investigated. inhibitory all these compounds depended upon the iron-free state molecules abolished by saturation with iron. No bactericidal effect transferrins observed at concentrations up to four times minimal bacteriostatic concentration. interaction legionella cell surface detected direct or indirect fluorescence assay, dialysis...
Coriander (Coriandruim sativum L., Apiaceae) is known for its antimicrobial activity and the aim of this study was to investigate effect essential oil (CDO) against multidrug resistant uropathogenic Escherichia coli (UPEC). CDO able inhibit growth UPEC strains propidium iodide uptake, - electron microscopy examination suggested that bacterial structural modifications occurred. The presence reduced MIC gentamicin. E.coli adhesion efficiency on cell monolayers abiotic surfaces not affected by...