A5030030059- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Urinary Tract Infections Management
- Bacterial Genetics and Biotechnology
- Reproductive tract infections research
- Viral gastroenteritis research and epidemiology
- Bacterial biofilms and quorum sensing
- Gut microbiota and health
- Enterobacteriaceae and Cronobacter Research
- Syphilis Diagnosis and Treatment
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Listeria monocytogenes in Food Safety
- Cervical Cancer and HPV Research
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Ear Surgery and Otitis Media
- Nasal Surgery and Airway Studies
- Macrophage Migration Inhibitory Factor
- Diet, Metabolism, and Disease
- Clostridium difficile and Clostridium perfringens research
- Hepatitis B Virus Studies
- Pneumonia and Respiratory Infections
- Antibiotic Use and Resistance
- Genital Health and Disease
Sapienza University of Rome
2012-2024
Istituto Superiore di Sanità
1999
Urinary tract infections (UTIs) are among the most common worldwide. Uropathogenic Escherichia coli (UPECs) main causative agent of UTIs. UPECs initially colonize human host adhering to bladder epithelium. Adhesion is followed by bacterial invasion urothelial epithelial cells where they can replicate form compact aggregates intracellular bacteria with biofilm-like properties. UPEC strains may persist within cells, thus acting as quiescent reservoirs (QIRs). It has been proposed that cell...
Adherent-invasive Escherichia coli (AIEC) have been implicated in the ethiopathogenesis of Crohn’s disease (CD). In this study, we analyzed a collection intestinal mucosa-associated E. isolates, presenting AIEC phenotypes, isolated from biopsies CD pediatric patients and non-inflammatory bowel diseases (IBD) controls, order to investigate their genetic phenotypic pathogenic features. A total 616 isolates four non-IBD controls were collected individually analyzed. For identification, adherent...
Urinary tract infections (UTIs) are mainly caused by uropathogenic
We have previously shown that integration of the virulence plasmid pINV into chromosome enteroinvasive Escherichia coli and Shigella flexneri makes these strains noninvasive (C. Zagaglia, M. Casalino, B. Colonna, C. Conti, A. Calconi, Nicoletti, Infect. Immun. 59:792-799, 1991). In this work, we studied transcription regulatory genes virB, virF, hns (virR) in wild-type E. HN280 its pINV-integrated derivative HN280/32. While virF is not affected by integration, virB severely reduced even if...
This work shows for the first time that Acinetobacter baumannii binds to carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), CEACAM5, and CEACAM6. binding significantly enhances A. internalization within alveolar host epithelia.
The ability of enteroinvasive Escherichia coli and Shigella flexneri to cause disease depends on the presence a large virulence plasmid (pINV). In this report we show that pHN280, pINV O135:K-:H- enteroivasive strain E. HN280, pWR100, S. serotype 5 M90T, are able integrate into specific site host chromosome. pINV-integrated HN280 M90T strains required methionine (Met-) grow in minimal medium, were noninvasive, did not produce contact-mediated hemolysin, had lost bind Congo red (Crb-) at 37...
Outer membrane protein A (OmpA) is a multifaceted predominant outer of Escherichia coli and other Enterobacteriaceae whose role in the pathogenesis various bacterial infections has recently been recognized. Here, OmpA on virulence Shigella flexneri investigated. An ompA mutant wild-type S. 5a strain M90T was constructed (strain HND92) it shown to be severely impaired cell-to-cell spreading since failed plaque HeLa cell monolayers. The lack significantly reduced levels IcsA while associated...
Proper protein localization is critical for bacterial virulence. PhoN2 a virulence-associated ATP-diphosphohydrolase (apyrase) involved in IcsA-mediated actin-based motility of S. flexneri. Herein, by analyzing ΔphoN2 mutant the flexneri strain M90T and generating phoN2::HA fusions, we show that PhoN2, periplasmic strictly localizes at poles, with strong preference old pole, pole where IcsA exposed, it required proper exposition. PhoN2-HA was found to be polarly localized both when...
Carbapenem-resistant Acinetobacter baumannii (CRAb) have emerged in recent decades as major causes of nosocomial infections. Resistance is mainly due to overexpression intrinsic and/or acquired carbapenemases, especially oxacillinases (OXA). In Italy, although the sequence type (ST) 2 and ST78 are most frequently detected, we recently reported ST632, a single locus variant ST2. Therefore, this study was aimed at unraveling common bacterial surface virulence factors involved pathogenesis...
Allergic rhinitis (AR) and adenoid hypertrophy (AH) are, in children, the main cause of partial or complete upper airway obstruction reduction airflow. However, limited data exist about impact increased resistance to airflow, on nasal microbial composition children with AR end AH. as well (AH), represent extremely common pathologies this population. Their known inflammatory is amplified when both coexist. In our study, microbiota anterior nares 75 pediatric subjects AR, AH conditions, was...
Adherent/invasive Escherichia coli (AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than healthy subjects. Since AIEC show a high percentage similarity to extraintestinal pathogenic E. (ExPEC), neonatal meningitis-associated (NMEC), uropathogenic (UPEC) strains, here we compared strain LF82 with UPEC isolate (strain EC73) assess whether would be able infect prostate cells as an...
Lactose-negative Escherichia coli strains were isolated at high frequency from children with diarrhea in Somalia during a 2-year study on diarrheal diseases. Sixty-four of these strains, considered to be representative sample, characterized for virulence factors, plasmid profiles, and antibiotic resistance. Of 5 recognized as enteroinvasive E. (they serotyped O135:K-:H-), 6 belonged classical enteropathogenic serotypes, 9 able adhere tissue culture cells (of these, 4 showed pattern localized...
Specific Escherichia coli strains have been associated to colorectal cancer, while no data are available on genotypic and phenotypic features of E. colonizing premalignant adenomatous polyps their pathogenic potential. This study was aimed at characterizing isolates collected from adjacent tissue in comparison with those normal mucosa. From colonoscopy biopsies, 1500 were retrieved genotyped; 272 characterized for phylogroup major traits (i.e., biofilm formation, motility, hemolysins,...
Satureja montana essential oil (SEO) presents a wide range of biological activities due to its high content active phytochemicals. In order improve the oil’s (EO) properties, in water nanoemulsions (NEs) composed SEO and Tween-80 were prepared, characterized, their antimicrobial antibiofilm properties assayed against Escherichia coli strains isolated from healthy chicken. Since surfactant composition can strongly influence NE features application field, ternary phase diagram was constructed...
Acinetobacter baumannii is a critical opportunistic pathogen associated with nosocomial infections. The high rates of antibiotic-resistance acquisition make most antibiotics ineffective. Thus, new medical countermeasures are urgently needed. Outer membrane proteins (OMPs) prime candidates for developing novel drug targets and antibacterial strategies. However, there substantial gaps in our knowledge A. OMPs. This study reports the impact OmpA-like protein on bacterial physiology virulence...
Chlamydia pneumoniae persistent infection has been implicated in the pathogenesis of several chronic inflammatory diseases including atherosclerosis, and we hypothesized that modulation apoptosis macrophages and/or T cells by C. may contribute to development such diseases. We therefore evaluated apoptosis, cytokine response, redox status human primary infected with pneumoniae. In addition, co-cultures were also carried out. Apoptosis, levels glutathione (GSH), disulfide (GSSG), tumour...
ABSTRACT The transcription of the virulence plasmid (pINV)-carried invasion genes Shigella flexneri and enteroinvasive Escherichia coli (EIEC) is induced at 37°C repressed 30°C. In this work, we report that O135: K−:H− EIEC strain HN280 S. SFZM53, M90T, 454, serotypes 4, 5, 2a, respectively, produce apyrase (ATP-diphosphohydrolase), product apy gene. addition, strains, but not strain, a nonspecific phosphatase encoded by phoN-Sf Both are pINV-carried loci whose contribution to pathogenicity...
We evaluated, in 415 patients with asymptomatic carotid atherosclerosis: (i) the prevalence of C. pneumoniae DNA atherosclerotic plaques and peripheral blood mononuclear cells (PBMC); (ii) distribution PBMC from same patients; (iii) correlation between circulating anti-chlamydial antibodies presence DNA. Overall, 160 174 specimens atherosclerosis were examined by ompA nested touchdown PCR for pneumoniae. In addition, was detected 81 plaque obtained patients. found 36.9% 40.2% (P=NS). With...