A5058039141- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Cancer Immunotherapy and Biomarkers
- Chemokine receptors and signaling
- Microtubule and mitosis dynamics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Monoclonal and Polyclonal Antibodies Research
- Reproductive System and Pregnancy
- Immune cells in cancer
- Ultrasound and Hyperthermia Applications
- Viral-associated cancers and disorders
- Mast cells and histamine
- Cancer, Hypoxia, and Metabolism
- CAR-T cell therapy research
- Sperm and Testicular Function
- Migraine and Headache Studies
- Glycosylation and Glycoproteins Research
- Photosynthetic Processes and Mechanisms
- Heavy Metal Exposure and Toxicity
- Bladder and Urothelial Cancer Treatments
- Endometriosis Research and Treatment
- Systemic Lupus Erythematosus Research
Marshall University
2025
Sapienza University of Rome
2013-2023
Italian Institute of Technology
2015
Istituto Pasteur
1999-2013
Policlinico Umberto I
2013
Otto-von-Guericke University Magdeburg
2009
Vita-Salute San Raffaele University
2009
Med-Storm Innovation (Norway)
2009
University of Brescia
2004
Istituto Neurologico Mediterraneo
2004
Cardiac myocytes have been traditionally regarded as terminally differentiated cells that adapt to increased work and compensate for disease exclusively through hypertrophy. However, in the past few years, compelling evidence has accumulated suggesting heart regenerative potential. Recent studies even surmised existence of resident cardiac stem cells, endothelial generating cardiomyocytes by cell contact or extracardiac progenitors cardiomyocytes, but these findings are still controversial....
CD4+CD25+Foxp3+ Tregs suppress autoimmune responses. In addition, they limit T cell responses during chronic infection, thereby minimizing cell–dependent immunopathology. We sought to investigate how are regulated in the livers of patients chronically infected with HCV, where control balance between an adequate protective immune response and suppression found that, despite accumulating proliferating at sites infection were relatively less expanded than CD4+CD25+Foxp3– effector cells. The...
We have studied the effects of steroid hormones, 17 beta-estradiol and dexamethasone, on relative proportion thymocyte expression CD4 (L3T4), CD8 (Ly-2), TCR IL-2R, used to identify different stages differentiation. After short-term in vivo treatment, a significant decrease number CD4+/CD8+, double positive subpopulation was observed parallel with proportional increase percentage CD4+/CD8- single positive, CD4-/CD8-, negative and, lesser extent, CD8+/CD4- subsets. Either treatment increased...
A new human retrovirus was isolated from a continuous cell line derived patient with CD4 + Tac - cutaneous T lymphoma/leukemia. This virus is related to but distinct leukemia/lymphoma types I and II (HTLV-I HTLV-II) immunodeficiency (HIV-1). With the use of fragment provirus cloned one leukemia, closely sequences were found in DNA tumor cells seven other patients same disease; these only distantly HTLV-I. The phenotype clinical course disease clearly distinguishable leukemia associated All...
Although decidual natural killer (NK) cell accumulation and vascular remodeling are critical steps to ensure successful pregnancy, the molecular mechanisms controlling these events poorly defined.Herein we analyzed whether chemerin, a recently identified chemoattractant involved in many pathophysiological processes, could be expressed uterine compartment regulate relevant for good outcome of pregnancy.Chemerin expression human primary culture stromal (ST) cells, extravillous trophoblast...
Increasing evidence indicates that cancer cell stress induced by chemotherapeutic agents promote antitumor immune responses and contribute to their full clinical efficacy. In this article, we identify the signaling events underlying chemotherapy-induced NKG2D DNAM-1 ligand expression on multiple myeloma (MM) cells. Our findings indicate sublethal doses of doxorubicin melphalan initiate a DNA damage response (DDR) controlling upregulation MM lines patient-derived malignant plasma cells in...
Abstract Several immune cell populations are involved in cartilage damage, bone erosion, and resorption processes during osteoarthritis. The purpose of this study was to investigate the role NK cells pathogenesis experimental osteoarthritis whether how neutrophils can regulate their synovial localization disease. Experimental elicited by intra-articular injection collagenase wild type Cxcr3−/− 8-wk old mice. To follow progression, thickening, osteophyte formation were measured...
Monocytes/macrophages are activated in several autoimmune diseases, including systemic sclerosis (scleroderma; SSc), with increased expression of interferon (IFN)-regulatory genes and inflammatory cytokines, suggesting dysregulation the innate immune response autoimmunity. In this study, we investigated whether lytic form Epstein-Barr virus (EBV) infection (infectious EBV) is present scleroderma monocytes contributes to their activation SSc. Monocytes were isolated from peripheral blood...
We phenotypically and functionally characterized a distinct CD56low natural killer cell subset based on CD16 expression levels in bone marrow peripheral blood of healthy children pediatric patients with acute lymphoblastic leukemia. Our findings demonstrate for the first time that CD56lowCD16low cells are more abundant than their frequency is further increased Bone compared CD56lowCD16high express lower inhibitory receptors, higher CD27, CD127, CD122, CD25, but undetectable CD57, suggesting...
Glioblastoma (GBM) is the most lethal and aggressive malignant primary brain tumor in adults. After surgical resection of tumor, patient typically should be subjected to chemotherapy (temozolomide, TMZ) concomitant radiotherapy. Since TMZ treatment does not lead complete remission often develops resistance, identification efficacious therapeutics strongly pursue. Among epigenetic players, H3K27 methyltransferase (MT) EZH2 (enhancer zeste homologue 2) has been found overexpressed or mutated...
Abstract Very late Ag (VLA)-3, VLA-4, and VLA-5, belonging to the beta-1 subfamily of integrins, have been recently identified as receptors for different binding regions fibronectin (FN). We detected VLA-4 but not VLA-3, on fresh CD3-, CD16+, CD56+ human NK cells by flow cytometry immunochemical analyses using mAb directed against beta-1, alpha-3, alpha-4, alpha-5 subunits. Binding assays, performed FN-coated plates, showed that specifically adhere FN their capacity is increased MgCl2 CaCl2....
Recognition of tumor cells by the immune system is a key step in cancer eradication. Melphalan an alkylating agent routinely used treatment patients with multiple myeloma (MM), but at therapeutic doses it leads to immunosuppressive state due lymphopenia. Here, we mouse model MM investigate ability vivo low melphalan modulate natural killer (NK) cell activity, which have been shown play major role control growth. was able enhance surface expression stress-induced NKG2D ligands RAE-1 and...
Abstract Granzyme B (GrB) is a serine proteinase known to be expressed by cytotoxic lymphocytes and induce, in presence of perforin (Pf), apoptosis target cells. Recently, GrB expression has been shown (often absence Pf) nonlymphoid cells, but its function not defined. In our study, we investigated Pf bladder cancer cell lines urothelial carcinoma (UC) tissues reverse transcription‐polymerase chain reaction (RT‐PCR), Western blot, ELISA, immunofluorescence immunohistochemistry. We also...
Autophagy has emerged as a key mechanism in the survival and function of T B lymphocytes, its activation was involved apoptosis resistance rheumatoid arthritis (RA). To investigate whether relationship between autophagy may impact response to therapy, we analyzed ex vivo spontaneous patients with RA subjected treatment anti-tumor necrosis factor (TNF) drugs vitro effects TNFα anti-TNF on cell fate. Peripheral blood mononuclear cells (PBMCs) from 25 treated were for levels marker LC3-II by...
CD69 represents a functional triggering molecule on activated NK and T cells, capable of inducing cytotoxic activity costimulating cytokine production. It belongs to the C-lectin type superfamily, its gene maps in complex, close other genes coding for receptors. CD94 / NKG2-A complex is inhibitory receptor non classical MHC class I HLA-E human cells. To investigate CD69-initiated signal transduction pathways, evaluate interference ability, we have generated transfectants expressing both...
The keratinocyte growth factor receptor (KGFR)/fibroblast 2b is activated by high‐affinity‐specific interaction with two different ligands, (KGF)/fibroblast (FGF)7 and FGF10/KGF2, which are characterized an opposite requirement of heparan sulfate proteoglycans heparin for binding to the receptor. We investigated here possible endocytic trafficking KGFR, induced ligands. Immunofluorescence immunoelectron microscopy analysis showed that KGFR internalization triggered either KGF or FGF10 occurs...