A5062614877- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Extracellular vesicles in disease
- Multiple Myeloma Research and Treatments
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- NF-κB Signaling Pathways
- Cytomegalovirus and herpesvirus research
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- Ubiquitin and proteasome pathways
- Estrogen and related hormone effects
- interferon and immune responses
- Immune Response and Inflammation
- Vitamin D Research Studies
- Histone Deacetylase Inhibitors Research
- Signaling Pathways in Disease
- Chemokine receptors and signaling
- Toxin Mechanisms and Immunotoxins
- Inflammatory mediators and NSAID effects
- Toxoplasma gondii Research Studies
- HIV Research and Treatment
- Peroxisome Proliferator-Activated Receptors
- Cholesterol and Lipid Metabolism
Istituto Pasteur
2016-2025
Sapienza University of Rome
2016-2025
Institut Pasteur
2016
Instituto Pasteur
2013
Cancer Institute (WIA)
2007
Tumori Foundation
1998
Istituto Neurologico Mediterraneo
1998
Science Applications International Corporation (United States)
1996-1997
National Cancer Institute
1995-1996
National Institutes of Health
1995
Interleukin 12 (IL-12), produced by myelomonocytic cells, plays a pivotal role in the development of T helper 1 (Th1) which are involved pathogenesis chronic inflammatory autoimmune disorders. 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] inhibits IL-12 production activated macrophages and dendritic thus providing novel interpretation to its immunosuppressive properties. 1,25(OH)2D3 significantly mRNA expression for both p35 p40 subunits acting at transcriptional level. The effect on promoter...
Abstract Cancer progression is continuously controlled by the immune system which can identify and destroy nascent tumor cells or inhibit metastatic spreading. However, its deregulated activity in microenvironment also promote favoring outgrowth of cancers capable escaping control, a process termed cancer immunoediting. This process, has been classified into three phases, i.e. “elimination”, “equilibrium” “escape”, influenced several cancer- microenvironment-dependent factors. Senescence...
1α,25-Dihydroxyvitamin D3 [1225-(OH)2D3] exerts several effects on the immune system, by regulating lymphocyte proliferation, differentiation of monocytes and secretion cytokines as IL-2, granulocyte-macrophage colony-stimulating factor IFN-γ in T cells. Here, we analyze effect 1,25-(OH)2D3 gene transcription. Transient transfection assays Jurkat cells indicate that activation promoter is down-regulated 1,25-(OH)2D3. This enhanced retinoid X receptor (RXR), a functional vitamin (VDR)...
Exosomes are a class of nanovesicles formed and released through the late endosomal compartment represent an important mode intercellular communication. The ability anticancer chemotherapy to enhance immunogenic potential malignant cells mainly relies on establishment cell death (ICD) release damage-associated molecular patterns (DAMPs). Here, we investigated whether genotoxic stress could promote exosomes from multiple myeloma (MM) studied immunomodulatory properties they exert NK cells,...
Abstract Natural killer (NK) cells are innate cytotoxic lymphocytes that play a key role in cancer immunosurveillance thanks to their ability recognize and kill cells. NKG2D is an activating receptor binds MIC ULBP molecules typically induced on damaged, transformed or infected The release of ligands (NKG2DLs) the extracellular milieu through protease‐mediated cleavage by vesicle (EV) secretion allows evade NKG2D‐mediated immunosurveillance. In this work, we investigated immunomodulatory...
Our group has previously reported that the nuclear factor Yin-Yang 1 (YY1), a ubiquitous DNA-binding protein, is able to interact with silencer element (BE) in gamma interferon (IFN-gamma) promoter region. In this study, we demonstrated YY1 can directly inhibit activity of IFN-gamma by interacting multiple sites promoter. cotransfection assays, expression vector significantly inhibited activity. Mutation binding site native was associated an increase Analysis DNA sequences revealed second...
Interferon-γ (IFN-γ) is an immunoregulatory cytokine expressed in large granular lymphocytes and T cells. However, the molecular mechanisms underlying IFN-γ gene transcription have not been fully defined. Here, we analyze responsible for inhibition of promoter activity by glucocorticoid hormone dexamethasone. Cotransfection assays performed Jurkat cells demonstrated that initial 108 base pairs was down-regulated presence Furthermore, utilizing electrophoretic mobility shift analysis,...
Genotoxic stress can promote antitumor NK cell responses by upregulating the surface expression of activating ligands on cancer cells. Moreover, a number studies suggested role for soluble group 2D in impairment tumor recognition and killing. We investigated whether genotoxic could release (MHC class I-related chain [MIC]A MICB), as well molecular mechanisms underlying this event human multiple myeloma (MM) Our results show that agents used therapy MM (i.e., doxorubicin melphalan)...
Increasing evidence indicates that cancer cell stress induced by chemotherapeutic agents promote antitumor immune responses and contribute to their full clinical efficacy. In this article, we identify the signaling events underlying chemotherapy-induced NKG2D DNAM-1 ligand expression on multiple myeloma (MM) cells. Our findings indicate sublethal doses of doxorubicin melphalan initiate a DNA damage response (DDR) controlling upregulation MM lines patient-derived malignant plasma cells in...
// Cinzia Fionda 1 , Maria Pia Abruzzese Alessandra Zingoni Francesca Cecere Elisabetta Vulpis Giovanna Peruzzi 2 Soriani Rosa Molfetta Rossella Paolini Rosaria Ricciardi 3 Teresa Petrucci Angela Santoni 1,4,* and Marco Cippitelli 1,* Department of Molecular Medicine, Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza University Rome, Italy Italiano di Tecnologia, CLNS@Sapienza, Division Hematology, Cellular Biotechnologies 4 Mediterraneo Neuroscienze Neuromed, Pozzilli, * These authors...
Anti-cancer immune responses may contribute to the control of tumors after conventional chemotherapy, and different observations have indicated that chemotherapeutic agents can induce resulting in cancer cell death immune-stimulatory side effects. Increasing experimental clinical evidence highlight importance natural killer (NK) cells toward multiple myeloma (MM), combination therapies able enhance activity NK against MM are showing promise treating this hematologic cancer. The epigenetic...
Abstract Treatment of multiple myeloma (MM) cells with sublethal doses genotoxic drugs leads to senescence and results in increased NK cell recognition effector functions. Herein, we demonstrated that doxorubicin- melphalan-treated senescent display expression IL15, a cytokine involved activation, proliferation, maturation. IL15 upregulation was evident at the mRNA protein level, both MM lines malignant plasma from patients’ bone marrow (BM) aspirates. However, detectable as soluble only...
The carefully orchestrated events that result in a protective immune response are coordinated to large extent by cytokines produced T helper 1 (Th1) and 2 (Th2) T-cell subsets, which two arms of the system. Th1 cells preferentially produce interleukin (IL-2), interferon gamma (IFN gamma), tumor necrosis factor (TNF), resulting cellular helps eliminate infected cells. In contrast, Th2 IL-4, IL-5, IL-6, IL-10 stimulate an antibody prevent from becoming infected. clinical progression several...
Modulation of the host immune system represents a promising therapeutic approach against cancer, including multiple myeloma. Recent findings indicate that NK group 2D (NKG2D)- and DNAX accessory molecule-1 (DNAM-1)-activating receptors play prominent role in tumor recognition elimination by cytotoxic lymphocytes, suggesting levels NKG2D DNAM-1 ligand expression on cells may be critical factor to improve response cancer. In this study, we tested effect 17-allylaminogeldanamycin radicicol,...
Abstract Engagement of NKG2D and DNAX accessory molecule-1 (DNAM-1) receptors on lymphocytes plays an important role for anticancer response represents interesting therapeutic target pharmacological modulation. In this study, we investigated the effect inhibitors targeting glycogen synthase kinase-3 (GSK3) expression DNAM-1 ligands in multiple myeloma (MM) cells. GSK3 is a pleiotropic serine–threonine kinase point convergence numerous cell-signaling pathways, able to regulate proliferation...