A5007969892- Force Microscopy Techniques and Applications
- Microbial Fuel Cells and Bioremediation
- Electrochemical sensors and biosensors
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- Diamond and Carbon-based Materials Research
- Metal and Thin Film Mechanics
- Electrochemical Analysis and Applications
- Mechanical and Optical Resonators
- Carbon Nanotubes in Composites
- Characterization and Applications of Magnetic Nanoparticles
- Polymer Surface Interaction Studies
- Near-Field Optical Microscopy
- Microbial Community Ecology and Physiology
- Graphene and Nanomaterials Applications
- Nanoparticle-Based Drug Delivery
- Molecular Junctions and Nanostructures
- Plant and Biological Electrophysiology Studies
- Electrocatalysts for Energy Conversion
- Magnetic properties of thin films
- Carbon and Quantum Dots Applications
- DNA and Nucleic Acid Chemistry
- Photoreceptor and optogenetics research
- Graphene research and applications
- Adhesion, Friction, and Surface Interactions
École Polytechnique Fédérale de Lausanne
2019-2024
École Polytechnique
2023-2024
Sapienza University of Rome
2014-2020
Centre National de la Recherche Scientifique
2017-2019
Institut de Physique et Chimie des Matériaux de Strasbourg
2017-2019
Université de Strasbourg
2017-2019
Clausthal University of Technology
2019
University of Rome Tor Vergata
2016
Italian Institute of Technology
2015
Université Laval
2015
Escherichia coli (E. coli) show limited extracellular electron transfer (EET) that compromises their use in bioelectronics. We enhance the EET E. by expressing an pathway spans inner and outer membranes of cell, including periplasmic space between. observe a 54% enhancement for engineered endogenous inner-membrane NapC NapB cytochromes under non-native conditions with outer-membrane MtrCAB complex from Shewanella oneidensis (S. oneidensis). The greatest enhancement, however, is observed...
Magnetic force microscopy (MFM) is an atomic (AFM) based technique in which AFM tip with a magnetic coating used to probe local fields the typical spatial resolution, thus allowing one acquire images reflecting properties of samples at nanoscale. Being well established tool for characterization recording media, superconductors and nanomaterials, MFM finding constantly increasing application study materials systems biological biomedical interest. After reviewing these latter applications,...
Abstract Living photovoltaics are microbial electrochemical devices that use whole cell–electrode interactions to convert solar energy electricity. The bottleneck in these technologies is the limited electron transfer between microbe and electrode surface. This study focuses on enhancing this by engineering a polydopamine (PDA) coating outer membrane of photosynthetic Synechocystis sp. PCC6803. provides conductive nanoparticle shell increase adhesion improve charge extraction. A combination...
Magnetic force microscopy (MFM) has been demonstrated as valuable technique for the characterization of magnetic nanomaterials. To be analyzed by MFM techniques, nanomaterials are generally deposited on flat substrates, resulting in an additional contrast images due to unavoidable heterogeneous electrostatic tip-sample interactions, which cannot easily distinguished from one. In order correctly interpret data, a method remove contributions is needed. this work, we propose new technique,...
Abstract Living photovoltaics represent a growing class of microbial devices that are based on whole cell–electrode interactions. The limited charge transfer at the interface represents significant bottleneck in realizing an efficient technology. This study focuses development poly(3,4‐ethylenedioxythiophene) (PEDOT)‐based electrodes electrosynthesized presence sodium dodecyl sulphate (SDS) dopant. Potentiodynamic and potentiostatic electrochemical techniques, as well scanning electron...
Titanium (Ti) is the most widely used metal in biomedical applications because of its biocompatibility; however, significant difference mechanical properties between Ti and surrounding tissues results stress shielding which detrimental for load-bearing tissues. In current study, to attenuate effect, a new processing route was developed. It aimed at growing thick poly(methyl methacrylate) (PMMA) layers grafted on substrates incorporate polymer component implants. However, currently available...
Detecting stiff nanoparticles buried in soft biological matrices by atomic force microscopy (AFM) based techniques represents a new frontier the field of scanning probe microscopies, originally developed as surface characterization methods. Here we report detection (magnetic) (NPs) internalized cells using contact resonance AFM (CR-AFM) employed potentially non-destructive subsurface tool. Magnetite (Fe3O4) NPs were microglial from cerebral cortices mouse embryos 18 days phagocytosis....
Abstract The discovery of extracellular electron conduits has spurred new applications in microbial electronics. Except for a limited number exoelectrogens, most microbes are surrounded by insulating membranes that impair transfer. This study focuses on the fabrication conductive polypyrrole (PPy) coating enhancing charge extraction. polymer deposition is characterized and optimized using combination potentiodynamic potentiostatic measurements as well scanning microscopy (SEM), energy...
The development of magnetic nanoparticles for biomedical applications requires a detailed characterization their properties, with relation not only to chemical structure, but also morphology and size. Magnetic force microscopy (MFM), thanks its nanometric lateral resolution capability detect weak fields, appears as powerful tool the properties single nanoparticles, together morphological characteristics. Nevertheless, application MFM quantitative measurements at nanoscale is still an open...
Abstract At the center of microbial bioelectricity applications lies critical need to express foreign heme proteins that are capable redirecting electron flux cell’s metabolism. This study presents bioengineered Synechocystis sp. PCC 6803 cells increased electrogenicity through introduction a genetic construct for cytochrome expression. We could demonstrate functional expression periplasmic MtrA decaheme c-type from Shewanella oneidensis , dissimilatory metal-reducing exoelectrogen, inside ....
Characterization of mechanical properties at the nanometer scale variable temperature is one main challenges in development polymer-based nanocomposites for application high environments. Contact resonance atomic force microscopy (CR-AFM) a powerful technique to characterize viscoelastic materials nanoscale. In this work, we demonstrate capability CR-AFM characterizing (i.e., storage and loss moduli, as well tangent) temperature. first illustrated on two polymeric reference samples, i.e.,...
Abstract The alkali-activation of titanium (Ti) surfaces performed in a heated sodium hydroxide (NaOH) aqueous solution, results porous layer rich hydroxyl (OH) groups, the structure and porosity which strongly depend on reaction time NaOH concentration used. In this study, polymerization initiator is covalently grafted alkali-activated Ti substrates by using phosphonic acid as coupling agent resulting are used scaffolds to drive growth tethered poly(methyl methacrylate) (PMMA) chains via...
Abstract Bioelectrochemical systems (BES) are promising for energy, sensing, environmental, and synthesis applications. Escherichia coli were previously bioengineered application in BES by introduction of extracellular electron transfer (EET) pathways. Inspired the metal-reducing (Mtr) pathway Shewanella oneidensis MR-1, several its cytochromes heterologously expressed E. , leading to increased EET rates successful BES. Besides direct S. MR-1 is known secrete flavins that act as redox...
The in-situ electrodeposition of polyaniline (PANI), one the most attractive conducting polymers (CP), has been monitored performing electrochemical atomic force microscopy (EC-AFM) experiments. electropolymerization PANI on a Pt working electrode observed cyclic voltammetry experiments and controlling evolution current flowing through surface, together with standard AFM image. principle potentialities this emerging technique are briefly reviewed factors limiting studying electrosynthesis...