A5039962080- Carbon Nanotubes in Composites
- Mesoporous Materials and Catalysis
- Nanoparticle-Based Drug Delivery
- Nanoparticles: synthesis and applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Zeolite Catalysis and Synthesis
- Recycling and Waste Management Techniques
- Microplastics and Plastic Pollution
- Catalytic Processes in Materials Science
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Field-Flow Fractionation Techniques
- Microfluidic and Bio-sensing Technologies
- Catalysis and Oxidation Reactions
- Protein purification and stability
- Nanotechnology research and applications
- Graphene research and applications
- SARS-CoV-2 and COVID-19 Research
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- SARS-CoV-2 detection and testing
- Graphene and Nanomaterials Applications
- Diatoms and Algae Research
- 3D Printing in Biomedical Research
Joint Research Centre
2016-2025
European Commission
2016-2022
Joint Research Centre
2017-2018
Directorate-General Joint Research Centre
2017
Consumers Union
2017
Don Carlo Gnocchi Foundation
2013-2016
International Flame Research Foundation
2015
Mylan (Switzerland)
2014-2015
University of Szeged
2001-2005
University of Namur
2003-2004
The implementation of the proposal from European Chemical Agency (ECHA) to restrict use nanoplastics (NP) and microplastics (MP) in consumer products will require reliable methods perform size mass-based concentration measurements. Analytical challenges arise at nanometre micrometre interface, e.g., 800 nm–10 µm, where techniques applicable scale reach their upper limit applicability approaches must be pushed lower limits detection. Herein, we compared performances nine analytical by...
Neutralizing antibodies that target the receptor-binding domain (RBD) of SARS-CoV-2 spike protein are among most promising approaches against COVID-191,2. A bispecific IgG1-like molecule (CoV-X2) has been developed on basis C121 and C135, two derived from donors who had recovered COVID-193. Here we show CoV-X2 simultaneously binds independent sites RBD and, unlike its parental antibodies, prevents detectable binding to cellular receptor virus, angiotensin-converting enzyme 2 (ACE2)....
Emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants diminishes the efficacy vaccines and antiviral monoclonal antibodies. Continued development immunotherapies vaccine immunogens resilient to viral evolution is therefore necessary. Using coldspot-guided antibody discovery, a screening approach that focuses on portions virus spike glycoprotein are both functionally relevant averse change, we identified human neutralizing antibodies highly conserved epitopes....
The availability of analytical methods for the characterization lipid nanoparticles (LNPs) in-vivo intracellular delivery nucleic acids is critical fast development innovative RNA therapies. In this study, protocols to measure (i) chemical composition, (ii) drug loading, (iii) particle size, concentration, and stability as well (iv) structure morphology were evaluated compared based on a comprehensive strategy linking key physical properties in-vitro efficacy toxicity. Furthermore,...
The use of nanotechnology in medical products has been demonstrated at laboratory scale, and many resulting nanomedicines are the translational phase toward clinical applications, with global market trends indicating strong growth sector coming years. translation clinic subsequent commercialization may require development new or adaptation existing standards to ensure quality, safety efficacy such products. This work addresses some identified needs, illustrates shortcomings currently used...
The measurement of physicochemical properties polydisperse complex biological samples, for example, extracellular vesicles, is critical to assess their quality, resulting from production and isolation methods. community gradually becoming aware the need combine multiple orthogonal techniques perform a robust characterization samples. Three pillars quality attribute EVs are sizing, concentration phenotyping. repeatable vesicle one key-challenges that requires further efforts, in order obtain...
Micro- and nanoplastic (pMP pNP, respectively) release is an emerging issue since these particles constitute a ubiquitous growing pollutant, which not only threatens the environment but may have potential consequences for human health. In particular, there concern about of secondary pMP pNP from degradation plastic consumer products. The phenomenon well-documented in relation to waste but, more recently, reports generated even during normal use food contact materials, such as water bottles,...
A comprehensive physicochemical characterization of heterogeneous nanoplastic (NPL) samples remains an analytical challenge requiring a combination orthogonal measurement techniques to improve the accuracy and robustness results. Here, batch methods, including dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), tunable resistive pulse sensing (TRPS), transmission electron microscopy (TEM), scanning (SEM), as well separation/fractionation methods such centrifugal liquid...
Quercetin-loaded nano-liposomes were prepared by high-pressure homogenization (HPH) at different pressures (up to 150 MPa) and number of passes 3) define the best processing conditions allowing lowest particle size highest encapsulation efficiency (EE). The process MPa for 1 pass was best, producing quercetin-loaded liposomes with 42% EE. Advanced techniques (multi-detector asymmetrical-flow field flow fractionation analytical ultracentrifugation combined transmission electron microscopy)...
Abstract When performing effect studies to investigate the impact of microplastic (MP) on cell lines, algae, or daphnia, it is advantageous if such experiments can be performed without use surfactants. The need for surfactants arises from fact that finely milled pristine MP particles generally are hydrophobic. Methods preparation larger amounts hydrophilic and hence artificially aged approaches their characterization high importance. Here we present methods age polyethylene terephthalate...
One of the possible functions photonic-crystal structure found on wing scales some butterflies is investigated. The optical and electron microscopic investigation two male butterflies-blue (colored) brown (discolored)-representing a sister species pair originating from different altitudes, revealed that blue color can be attributed unambiguously to fine, spongelike medium, called "pepper-pot structure," present between ridges cross ribs in colored butterfly. Only traces this discolored Other...
Analytical ultracentrifugation (AUC) is a powerful tool for the study of particle size distributions and interactions with high accuracy resolution. In this work, we show how analysis sedimentation velocity data from AUC can be used to characterize nanocarrier drug delivery systems in nanomedicine. Nanocarrier distribution ratio free versus nanoparticle-encapsulated commercially available liposomal doxorubicin formulation are determined using interference absorbance based measurements...
Silver nanoparticles (AgNPs) may be synthesized by many different methods, with those based on the thermal reduction of silver salts citric acid or acid/tannic being amongst most commonly used. These although widely used and technically simple, can produce particles in which size, polydispersivity morphology vary greatly. In this work nearly mono-dispersed spherical AgNPs have been via a one-step method using sodium citrate varying quantities Tannic Acid (TA), was thermally conditioned prior...
Titanium dioxide is a white colourant authorised as food additive E171 in the EU and applied range of products. Currently specifications for do not refer to characterisation particle size distribution; however, this may be requested near future. Only few studies have been published date reporting data on distribution grade titanium dioxide. The aim study was characterise particles contained eight confectionery products pristine samples used each This allowed direct comparison both extracted...
Liposomal formulations for the treatment of cancer and other diseases are most common form nanotechnology enabled pharmaceuticals (NEPs) submitted market approval in clinical application today. The accurate characterization their physical-chemical properties is a key requirement; particular, size, size distribution, shape, stability among that regulators identify as critical quality attributes. Here we develop validate an optimized method, based on multi-detector asymmetrical-flow field flow...
Validation of analytical methods for measurements microplastics (MP) is severely hampered because a general lack reference materials, RM. There great need to develop such materials. This study presents concept three-component kit with immobilised MP in solid NaCl, surfactant and clean water that can be applied the production many types RMs. As proof concept, an RM polyethylene terephthalate (PET) particles was prepared evaluated its homogeneity. The ranged from 30 μm (Feretmin) about 200...
Microplastic pollution represents a global problem with negative impacts on aquatic environment and organisms' health. To date, most of the laboratory toxicological studies microplastics (MPs) have made use single commercial micro nano-polymers, which do not reflect heterogeneity environmental MPs. improve relevance hazard assessment, micrometer-sized plastic particles miscellaneous non-reusable waste plastics, size <100 µm <50 (waste microplastics, wMPs), were characterized by microscopic...
Curcumin-loaded polymeric micelles composed of poly(ethylene glycol)-b-poly(N-2-benzoyloxypropyl methacrylamide) (mPEG-b-p(HPMA-Bz)) were prepared to solubilize and improve the pharmacokinetics curcumin. by a nanoprecipitation method using mPEG5kDa-b-p(HPMA-Bz) copolymers with varying molecular weight hydrophobic block (5.2, 10.0, 17.1 kDa). At equal curcumin loading, mPEG5kDa-b-p(HPMA-Bz)17.1kDa showed better retention in both phosphate-buffered saline (PBS) plasma at 37 °C than based on...
Heamatopoietic cancer cells from patients were objectively and accurately recognized by high-resolution Raman imaging their characteristic spectra.
Abstract Plastic particulates in the environment pose an increasing concern for regulatory bodies due to their potential risk higher organisms (including humans) as they enter food chain. Nanoplastics (defined here smaller than 1 μm) are particularly challenging detect and analyze at environmentally relevant concentrations biological matrices. The tunicate Ciona Robusta is effective bioindicator microplastics nanoplastic contamination marine environment, its capacity filter substantial...