A5075627896- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Tuberculosis Research and Epidemiology
- SARS-CoV-2 detection and testing
- Mycobacterium research and diagnosis
- Biochemical and Molecular Research
- Vaccine Coverage and Hesitancy
- Cancer therapeutics and mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Bacillus and Francisella bacterial research
- Periodontal Regeneration and Treatments
- COVID-19 and healthcare impacts
- Dental Implant Techniques and Outcomes
- Bone Tissue Engineering Materials
- COVID-19 epidemiological studies
- Immunotherapy and Immune Responses
- Herpesvirus Infections and Treatments
- Long-Term Effects of COVID-19
- Virus-based gene therapy research
- Poxvirus research and outbreaks
- Bacterial Infections and Vaccines
- Dental materials and restorations
- Infection Control and Ventilation
- RNA modifications and cancer
- Mosquito-borne diseases and control
University of Pavia
2019-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2021-2024
Policlinico San Matteo Fondazione
2021-2023
Istituto Universitario di Studi Superiori di Pavia
2019-2020
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
2020
Istituto di Biologia e Biotecnologia Agraria
2019
University of Naples Federico II
2016-2017
Federico II University Hospital
2015-2017
The recent emergence of the Omicron variant has raised concerns on vaccine efficacy and urgent need to study more efficient vaccination strategies. Here we observed that an mRNA booster in individuals vaccinated with two doses inactivated significantly increased plasma level specific antibodies bind receptor-binding domain (RBD) or spike (S) ectodomain (S1 + S2) both G614 variants, compared homologous vaccine. RBD- S-specific IgG virus neutralization titers against variants concern...
Neutralizing antibodies that target the receptor-binding domain (RBD) of SARS-CoV-2 spike protein are among most promising approaches against COVID-191,2. A bispecific IgG1-like molecule (CoV-X2) has been developed on basis C121 and C135, two derived from donors who had recovered COVID-193. Here we show CoV-X2 simultaneously binds independent sites RBD and, unlike its parental antibodies, prevents detectable binding to cellular receptor virus, angiotensin-converting enzyme 2 (ACE2)....
The COVID-19 pandemic is caused by the betacoronavirus SARS-CoV-2. In November 2021, Omicron variant was discovered and immediately classified as a of concern (VOC), since it shows substantially more mutations in spike protein than any previous variant, especially receptor-binding domain (RBD). We analyzed binding RBD to human angiotensin-converting enzyme-2 receptor (ACE2) ability sera from patients or vaccinees comparison Wuhan, Beta, Delta variants.
Mosquito-borne West Nile virus (WNV) infection is benign in most individuals but can cause encephalitis <1% of infected individuals. We show that ∼35% patients hospitalized for WNV disease (WNVD) six independent cohorts from the EU and USA carry auto-Abs neutralizing IFN-α and/or -ω. The prevalence these antibodies highest with (∼40%), silent as low general population. odds ratios WNVD relative to those without them population range 19.0 (95% CI 15.0–24.0, P value <10–15) only...
The emergence of Omicron lineages and descendent subvariants continues to present a severe threat the effectiveness vaccines therapeutic antibodies. We have previously suggested that an insufficient mucosal immunoglobulin A (IgA) response induced by mRNA is associated with surge in breakthrough infections. Here, we further show intramuscular and/or inactivated cannot sufficiently boost secretory IgA uninfected individuals, particularly against variant. thus engineered characterized...
Vaccine breakthrough SARS-CoV-2 infection has been monitored in 3720 healthcare workers receiving 2 doses of BNT162b2. is detected 33 subjects, with a 100-day cumulative incidence 0.93%. protection against acquisition 83% (95%CI: 58-93%) the overall population and 93% 69-99%) SARS-CoV-2-experienced when compared non-vaccinated control group from same Institution, which occurs 20/346 subjects (100-day incidence: 5.78%). The symptomatic 16 (48%) vaccinated vs 17 (85%) controls (p = 0.01). All...
Since the breakout of COVID-19, mutated forms severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have shown enhanced rates transmission and adaptation to humans. The variants concern (VOC), designated Alpha, Beta, Gamma, Delta, Omicron emerged independent one another, in turn rapidly became dominant. success each VOC, as well virus fitness, were enabled by altered intrinsic functional properties and, reasonably, antigenicity changes, conferring ability evade a primed immune...
Background: Titanium dental implants are today widely used with osseointegration mainly dependently on the implant surface properties. Different processing routes lead to different characteristics resulting, of course, in situ behaviors implants. Materials: The effect treatments, whether mechanical or chemical, morphology titanium were investigated. To this aim, various experimental methods, including roughness analysis as well scanning electron microscope (SEM) observations, applied....
Tuberculosis (TB) is one of the major causes deaths related to antimicrobial resistance worldwide because spread Mycobacterium tuberculosis multidrug resistant (MDR and XDR) clinical isolates. To fight MDR XDR tuberculosis, three new antitubercular drugs, bedaquiline (BDQ), delamanid, pretomanid were approved for their use in setting. Unfortunately, BDQ quickly acquired two main mechanisms resistance, consisting mutations both atpE gene, encoding target, Rv0678, coding repressor MmpS5-MmpL5...
Abstract Monkeypox virus (MPXV) is a zoonotic disease endemic in the rainforest countries of Central and West Africa. Understanding immune response zoonosis fundamental to prevent contrast viral spreading. MPXV close relative Variola (smallpox) vaccination with vaccinia gives approximatively 85% protection against MPXV. With emergence recent outbreak, JYNNEOS vaccine has been proposed individuals at high‐risk exposure. Comparative data on vaccinated or infected subjects are still limited....
•In SARS-CoV-2-naïve subjects, spike-specific T-cell response was significantly increased after the second dose of vaccine•In patients treated with chemo-immunotherapy, seems to be lower borderline statistical significance•Between naïve and experienced patients, no differences were reported in terms CD4- CD8-specific response•In level SARS-CoV-2 neutralizing antibodies reached a higher median 1 : 80 dose•No COVID-19 cases documented throughout period study BackgroundVery few cancer enrolled...
To assess the humoral and cell-mediated response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) elicited by mRNA BNT162b2 vaccine in SARS-CoV-2-experienced -naive subjects against a reference strain SARS-CoV-2 variants.The (including neutralizing antibodies) T-cell-mediated 145 healthcare workers (both naive positive for previous infection) were evaluated. In subset of subjects, effect variants on antibody level was also investigated.Overall, 125/127 (98.4%) developed both...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has severely impacted on public health, mainly immunosuppressed patients, including solid organ transplant recipients. Vaccination represents a valuable tool for the prevention of SARS-CoV-2 infection, and immunogenicity mRNA vaccines been evaluated in transplanted patients. In this study, we investigated role third dose BNT162b2 vaccine cohort kidney recipients, analyzing both humoral cell-mediated responses. We...
•All subjects but one reached detectable IgG levels 3 weeks after the third dose of SARS-CoV-2 BNT162b2 messenger RNA vaccine.•A median 16-fold increase NT Ab titer was observed in patients without previous COVID-19.•The cell-mediated immune response significantly higher SARS-CoV-2-naïve booster.•Median level Abs 32-fold lower against Omicron compared to wild-type strain. BackgroundAlthough a full course coronavirus disease 2019 (COVID-19) vaccine is effective cancer patients, duration...
Platelet-derived factors are biomaterials that might accelerate healing process in oral, maxillofacial, and several other applications. Release of specific by platelet concentrates is critical to achieving a successful outcome. Here, we have shown platelet-rich fibrin (PRF) clots were beneficial sources leukocytes, which may directly affect the release chemokines growth factors. When compared with standard leukocyte-PRF (L-PRF), experimental low-force modified procedure [defined as...
We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian PV10734 (D614G, B.1.1) and Alpha (lineage B.1.1.7)) were able infect monocytes (MN) monocyte-derived macrophages (MDM) these infected cells may, in turn, be vectors infection. For this purpose, we designed an vitro study following the evolution MN MDM infection at different time points order confirm permissive for replication. Finally, investigated whether, regardless viral replication,...
Avermectins are macrocyclic lactones with anthelmintic activity. Recently, they were found to be effective against Mycobacterium tuberculosis, which accounts for one third of the worldwide deaths from antimicrobial resistance. However, their anti-mycobacterial mode action remains elucidated. The activity selamectin was determined a panel M. tuberculosis mutants. Two strains carrying mutations in DprE1, decaprenylphosphoryl-β-D-ribose oxidase involved synthesis mycobacterial arabinogalactan,...
Starting from the analysis of hypothetical binding mode our previous furan-based hit (I), we successfully achieved objective to replace nitro moiety, leading disclosure a new lead exhibiting strong activity against MbtI. Our best candidate 1 h displayed Ki 8.8 µM and its antimycobacterial (MIC99 = 250 µM) is conceivably related mycobactin biosynthesis inhibition. These results support hypothesis that 5-phenylfuran-2-carboxylic derivatives are promising class MbtI inhibitors.
The Mg2+-dependent Mycobacterium tuberculosis salicylate synthase (MbtI) is a key enzyme involved in the biosynthesis of siderophores. Because iron essential for survival and pathogenicity microorganism, this protein constitutes an attractive target antitubercular therapy, also considering absence homologous enzymes mammals. An extension structure–activity relationships our furan-based candidates allowed us to disclose most potent competitive inhibitor known date (10, Ki = 4 μM), which...
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants has raised concerns about reduced vaccine effectiveness and the increased risk infection, while repeated homologous booster shots are recommended for elderly immunocompromised individuals, they cannot completely protect against breakthrough infections. In our previous study, we assessed immunogenicity an adenovirus-based expressing SARS-CoV-2 S1 (Ad5.S1) in mice, which induced robust humoral cellular...