A5039460637- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- COVID-19 Clinical Research Studies
- Viral Infections and Outbreaks Research
- SARS-CoV-2 detection and testing
- Hepatitis Viruses Studies and Epidemiology
- Virus-based gene therapy research
- Zoonotic diseases and public health
- Hepatitis B Virus Studies
- Fire effects on ecosystems
- Bat Biology and Ecology Studies
- Rabies epidemiology and control
- Mosquito-borne diseases and control
- Liver Disease and Transplantation
- Plant Virus Research Studies
- Poxvirus research and outbreaks
- Bacillus and Francisella bacterial research
- Animal Diversity and Health Studies
- Animal Disease Management and Epidemiology
- Virology and Viral Diseases
- Computational Drug Discovery Methods
- Respiratory viral infections research
- Bacteriophages and microbial interactions
Kunming Institute of Zoology
2022-2025
Chinese Academy of Sciences
2015-2024
Wuhan Institute of Virology
2015-2024
University of Chinese Academy of Sciences
2017-2024
Duke-NUS Medical School
2017-2023
Dali University
2017
Yunnan Institute of Endemic Diseases Control and Prevention
2017
Wuhan University
2017
EcoHealth Alliance
2017
Abstract Since the outbreak of severe acute respiratory syndrome (SARS) 18 years ago, a large number SARS-related coronaviruses (SARSr-CoVs) have been discovered in their natural reservoir host, bats 1–4 . Previous studies shown that some bat SARSr-CoVs potential to infect humans 5–7 Here we report identification and characterization new coronavirus (2019-nCoV), which caused an epidemic Wuhan, China. The epidemic, started on 12 December 2019, had 2,794 laboratory-confirmed infections...
In December 2019, a novel coronavirus (2019-nCoV) caused an outbreak in Wuhan, China, and soon spread to other parts of the world. It was believed that 2019-nCoV transmitted through respiratory tract then induced pneumonia, thus molecular diagnosis based on oral swabs used for confirmation this disease. Likewise, patient will be released upon two times negative detection from swabs. However, many coronaviruses can also oral–fecal route by infecting intestines. Whether infected patients carry...
The 2002-3 pandemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV) was one of the most significant public health events in recent history. An ongoing outbreak Middle East suggests that this group viruses remains a key threat and their distribution is wider than previously recognized. Although bats have been suggested to be natural reservoirs both viruses, attempts isolate progenitor virus SARS-CoV from unsuccessful. Diverse SARS-like coronaviruses (SL-CoVs) now reported...
A large number of SARS-related coronaviruses (SARSr-CoV) have been detected in horseshoe bats since 2005 different areas China. However, these bat SARSr-CoVs show sequence differences from SARS coronavirus (SARS-CoV) genes (S, ORF8, ORF3, etc) and are considered unlikely to represent the direct progenitor SARS-CoV. Herein, we report findings our 5-year surveillance a cave inhabited by multiple species Yunnan Province, The full-length genomes 11 newly discovered SARSr-CoV strains, together...
Since the SARS outbreak 18 years ago, a large number of severe acute respiratory syndrome related coronaviruses (SARSr-CoV) have been discovered in their natural reservoir host, bats. Previous studies indicated that some those bat SARSr-CoVs potential to infect humans. Here we report identification and characterization novel coronavirus (nCoV-2019) which caused an epidemic humans, Wuhan, China. The epidemic, started from December 12th, 2019, has 198 laboratory confirmed infections with three...
Cross-species transmission of viruses from wildlife animal reservoirs poses a marked threat to human and health
We report the isolation and characterization of a novel bat coronavirus which is much closer to severe acute respiratory syndrome (SARS-CoV) in genomic sequence than others previously reported, particularly its S gene. Cell entry susceptibility studies indicated that this virus can use ACE2 as receptor infect animal human cell lines. Our results provide further evidence origin SARS-CoV highlight likelihood future emergence humans.
Abstract The 2019 novel coronavirus (SARS-CoV-2) outbreak is a major challenge for public health. SARS-CoV-2 infection in human has broad clinical spectrum ranging from mild to severe cases, with mortality rate of ~6.4% worldwide (based on World Health Organization daily situation report). However, the dynamics viral infection, replication and shedding are poorly understood. Here, we show that Rhesus macaques susceptible by SARS-CoV-2. After intratracheal inoculation, first peak RNA was...
ABSTRACT Increasing data indicate that bats harbor diverse viruses, some of which cause severe human diseases. In this study, sequence-independent amplification and high-throughput sequencing (Solexa) were applied to the metagenomic analysis viruses in bat fecal samples collected from 6 locations China. A total 8,746,417 reads with a length 306,124,595 bp obtained. Among these reads, 13,541 (0.15%) had similarity phage sequences 9,170 (0.1%) eukaryotic virus sequences. 129 assembled contigs...
SUMMARY A new coronavirus (CoV) identified as COVID-19 virus is the etiological agent responsible for 2019-2020 viral pneumonia outbreak that commenced in Wuhan 1–4 . Currently there no targeted therapeutics and effective treatment options remain very limited. In order to rapidly discover lead compounds clinical use, we initiated a program of combined structure-assisted drug design, virtual screening high-throughput identify leads target main protease (M pro ). M key CoV enzyme, which plays...
Abstract SARS-CoV-2 induced marked lymphopenia in severe patients with COVID-19. However, whether lymphocytes are targets of viral infection is yet to be determined, although RNA or antigen has been identified T cells from patients. Here, we confirmed that could detected patient peripheral blood (PBCs) postmortem lung cells, and the infectious virus also antigen-positive PBCs. We next prove infects lymphocytes, preferably activated CD4 + vitro. Upon infection, RNA, subgenomic protein...
The emergence of Omicron lineages and descendent subvariants continues to present a severe threat the effectiveness vaccines therapeutic antibodies. We have previously suggested that an insufficient mucosal immunoglobulin A (IgA) response induced by mRNA is associated with surge in breakthrough infections. Here, we further show intramuscular and/or inactivated cannot sufficiently boost secretory IgA uninfected individuals, particularly against variant. thus engineered characterized...
ABSTRACT Middle East respiratory syndrome coronavirus (MERS-CoV) has represented a human health threat since 2012. Although several MERS-related CoVs that belong to the same species as MERS-CoV have been identified from bats, they do not use receptor, dipeptidyl peptidase 4 (DPP4). Here, we screened 1,059 bat samples at least 30 collected in different regions south China and 89 strains of lineage C betacoronaviruses, including Tylonycteris pachypus HKU4 , Pipistrellus pipistrellus HKU5 CoVs....
Coronaviruses (CoVs) of bat origin have caused two pandemics in this century. Severe acute respiratory syndrome (SARS)-CoV and Middle East (MERS)-CoV both originated from bats, it is highly likely that coronaviruses will cause future outbreaks. Active surveillance urgent essential to predict mitigate the emergence these viruses humans. Next-generation sequencing (NGS) currently preferred methodology for virus discovery ensure unbiased CoVs, considering their high genetic diversity. However,...
The emerging SARS-CoV-2 infection associated with the outbreak of viral pneumonia in China is ongoing worldwide. There are no approved antiviral therapies to treat this disease. Here we examined abilities three broad-spectrum compounds gemcitabine, lycorine and oxysophoridine against cell culture. We found that all tested inhibited replication Vero-E6 cells at noncytotoxic concentrations. effect gemcitabine was suppressed efficiently by cytidine nucleosides. Additionally, combination had an...