A5075159935- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Glycosylation and Glycoproteins Research
- interferon and immune responses
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Immune Response and Inflammation
- Animal Virus Infections Studies
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Viral Infections and Vectors
- Cytokine Signaling Pathways and Interactions
- Lipoproteins and Cardiovascular Health
- Computational Drug Discovery Methods
- COVID-19 Clinical Research Studies
- Viral Infections and Outbreaks Research
- Polyomavirus and related diseases
- Aquaculture disease management and microbiota
- Virus-based gene therapy research
- Cancer-related Molecular Pathways
- Galectins and Cancer Biology
BGI Research
2025
BGI Group (China)
2019-2023
First Affiliated Hospital of Guangzhou Medical University
2022
State Key Laboratory of Respiratory Disease
2022
Guangzhou Medical University
2022
China National GeneBank
2019-2022
Wuhan Institute of Virology
2013-2015
Chinese Academy of Sciences
2013-2015
We report the isolation and characterization of a novel bat coronavirus which is much closer to severe acute respiratory syndrome (SARS-CoV) in genomic sequence than others previously reported, particularly its S gene. Cell entry susceptibility studies indicated that this virus can use ACE2 as receptor infect animal human cell lines. Our results provide further evidence origin SARS-CoV highlight likelihood future emergence humans.
Chondrichthyan (cartilaginous fish) occupies a key phylogenetic position and is important for investigating evolutionary processes of vertebrates. However, limited whole genomes impede our in-depth knowledge issues such as chromosome evolution immunity. Here, we report the chromosome-level genome white-spotted bamboo shark. Combing it with other shark genomes, reconstructed 16 ancestral chromosomes illustrate dynamic rearrangement process. We found that genes on 13 fast-evolving can be...
Bats have been identified as natural reservoirs of many viruses, including reoviruses. Recent studies demonstrated the interspecies transmission bat reoviruses to humans. In this study, we report isolation and molecular characterization six strains mammalian orthoreovirus (MRV) from Hipposideros Myotis spp. These isolates were grouped into MRV serotype 1, 2 or 3 based on sequences S1 gene, which encodes outer coat protein s1. Importantly, found that three shared high similarity with MRVs...
SARS-CoV-2 Omicron variants feature highly mutated spike proteins with extraordinary abilities in evading antibodies isolated earlier the pandemic. Investigation of memory B cells from patients primarily breakthrough infections Delta variant enables isolation a number neutralizing cross-reactive to heterologous concern (VOCs) including (BA.1-BA.4). Structural studies identify altered complementarity determining region (CDR) amino acids and unusual heavy chain CDR2 insertions respectively two...
The development of shark single domain antibodies (sdAbs) is hindered by the high cost and tediousness large-sized farming. Here, we demonstrated white-spotted bamboo sharks (Chiloscyllium plagiosum) being cultivated commercially as a promising small animal model to produce sdAbs. We found that immunoglobulin new antigen receptor (IgNAR) presented in genome, transcriptome, plasma. Four complete IgNAR clusters including variable domains (vNARs) were discovered germline, Variable-Joining pair...
CD4+ T cells are crucial in cytomegalovirus (CMV) infection, but their role infection remains unclear. The heterogeneity and potential functions of CMVpp65-reactivated cell subsets isolated from human peripheral blood, as well interactions, were analyzed by single-cell RNA-seq receptor (TCR) sequencing. Tregs comprised the largest population these reactivated cells, analysis Treg gene expression showed transcripts associated with both inflammatory inhibitory functions. detailed phenotypes...
Abstract Background The advent of proprotein convertase subtilisin/kexin type 9 (PCSK9)–inhibiting drugs have provided an effective, but extremely expensive treatment for the management low density lipoprotein (LDL). Our aim was to explore a cost‐effective application camelid anti‐PCSK9 single domain antibodies (sdAbs), which are high variable regions heavy chain (VHHs), as human PCSK9 (hPCSK9) inhibitor. One female llama immunized with hPCSK9. Screening affinity VHHs carried out based on...
Nanobodies, also known as VHHs, originate from the serum of Camelidae. Nanobodies have considerable advantages over conventional antibodies, including smaller size, more modifiable, and deeper tissue penetration, making them promising tools for immunotherapy antibody-drug development. A high-throughput nanobody screening platform is critical to rapid development nanobodies. To date, droplet-based microfluidic systems exhibited improved performance compared traditional phage display...
The 0.8-Mb Ig new Ag receptor (IgNAR) region of the whitespotted bamboo shark (Chiloscyllium plagiosum) is incompletely assembled in Chr_44 reference genome. Here we used Cas9-assisted targeting chromosome segments (CATCH) to enrich 2 Mb IgNAR loci and sequenced it by PacBio next-generation sequencing. A fragment >3.13 was isolated intact from RBCs sharks. target enriched 245.531-fold, sequences had up 94% coverage with a 255× mean depth. Compared previously published sequences, 20 holes...
Avian influenza A virus H5N1 is a highly pathogenic and persistently major threat to global health. Vaccines antibodies targeting hemagglutinin (HA) protein are the primary management strategies for epidemic virus. Although camelids possess unique immunological features, immune response induced by specific antigens has not yet been thoroughly investigated. Herein, we immunized an alpaca with HA antigen of performed single-cell transcriptome profiling analysis longitudinal peripheral blood...
Abstract Cartilaginous fishes have a very high phenotypical diversity, phenomenon for which the mechanisms been largely unexplored. Here, we report genome of white-spotted bamboo shark as first chromosome-level assembly cartilaginous fish. Using this genome, illustrated dynamic chromosome rearrangement process in shark, resulted formation 13 chromosomes, all were sparsely distributed with conserved genes and fast-evolving. We found fast-evolving chromosomes to be enriched immune-related two...
Abstract PCSK9 is an effective target for lowering LDL-c. Previously, a camelid-human chimeric heavy chain antibody VHH-B11-Fc targeting human was designed. It had potent hypolipidemic effect. However, the nanobody VHH-B11 interacts with at low affinity, while camelid VHH exhibits some immunogenicity. Moreover, interacting epitope yet to be identified, although shown have distinct hPCSK9-binding epitopes Evolocumab. This might impede molecule’s progress from bench bedside. In present study,...
ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the pandemic of disease 2019 (COVID-19). Great international efforts have been put into development prophylactic vaccines and neutralizing antibodies. However, knowledge about B cell immune response induced by SARS-CoV-2 virus is still limited. Here, we report a comprehensive characterization dynamics immunoglobin heavy chain (IGH) repertoire in COVID-19 patients. By using next-generation sequencing technology,...
Abstract Currently circulating SARS-CoV-2 Omicron variants feature highly mutated spike proteins with extraordinary abilities in evading acute-infection-induced germline antibodies isolated earlier the pandemic. We identified that memory B cells from Delta variant breakthrough-infection patients expressed more extensive somatic hypermutations (SHMs) allowing isolation of a number broadly neutralizing activities against heterologous concerns (VOCs) including variant. Structural studies SHM...