A5076498328- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Plant-derived Lignans Synthesis and Bioactivity
- SARS-CoV-2 and COVID-19 Research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Phytochemistry and Biological Activities
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Toxin Mechanisms and Immunotoxins
- Traditional and Medicinal Uses of Annonaceae
- HIV/AIDS Research and Interventions
- Natural product bioactivities and synthesis
- T-cell and B-cell Immunology
- interferon and immune responses
- Hepatitis C virus research
- Phytochemical compounds biological activities
- Click Chemistry and Applications
- Herpesvirus Infections and Treatments
- Mosquito-borne diseases and control
- Pneumocystis jirovecii pneumonia detection and treatment
- Cholinesterase and Neurodegenerative Diseases
- Transgenic Plants and Applications
- Bioactive Natural Diterpenoids Research
- Immunotherapy and Immune Responses
Kunming Institute of Zoology
2016-2025
Chinese Academy of Sciences
2016-2025
University of Hong Kong
2016-2025
University of Chinese Academy of Sciences
2016-2025
Soochow University
2016-2025
Yunnan University of Traditional Chinese Medicine
2024
Huzhou University
2024
Chinese University of Hong Kong
2010-2023
University of Science and Technology of China
2014-2022
Center for Excellence in Brain Science and Intelligence Technology
2020-2021
The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continually poses serious threats to global public health. main protease (Mpro) of SARS-CoV-2 plays a central role in viral replication. We designed and synthesized 32 new bicycloproline-containing Mpro inhibitors derived from either boceprevir or telaprevir, both which are approved antivirals. All compounds inhibited activity vitro, with 50% inhibitory concentration values ranging 7.6 748.5 nM....
Chinese tree shrews (Tupaia belangeri chinensis) possess many features valuable in animals used as experimental models biomedical research. Currently, there are numerous attempts to employ for a variety of human disorders: depression, myopia, hepatitis B and C virus infections, hepatocellular carcinoma, name few. Here we present publicly available annotated genome sequence the shrew. Phylogenomic analysis shrew other mammalians highly support its close affinity primates. By characterizing...
The zinc-finger antiviral protein (ZAP) was originally identified as a host factor that inhibits the replication of Moloney murine leukemia virus. Here we report ZAP HIV-1 infection by promoting degradation specific viral mRNAs. Overexpression rendered cells resistant to in expression level-dependent manner, whereas depletion endogenous enhanced infection. Both human and rat inhibited propagation replication-competent HIV-1. specifically targeted multiply spliced but not unspliced or singly...
Abstract The 2019 novel coronavirus (SARS-CoV-2) outbreak is a major challenge for public health. SARS-CoV-2 infection in human has broad clinical spectrum ranging from mild to severe cases, with mortality rate of ~6.4% worldwide (based on World Health Organization daily situation report). However, the dynamics viral infection, replication and shedding are poorly understood. Here, we show that Rhesus macaques susceptible by SARS-CoV-2. After intratracheal inoculation, first peak RNA was...
Coronavirus disease 2019 (COVID-19) is a global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The models that can accurately resemble human-relevant responses to viral infection are lacking. Here, biomimetic human model on chip allows recapitulate lung injury and immune induced SARS-CoV-2 in vitro at organ level created. This alveolar reproduce the key features of alveolar-capillary barrier coculture epithelium, microvascular endothelium, circulating cells...
Abstract SARS-CoV-2 is the underlying cause for COVID-19 pandemic. Like most enveloped RNA viruses, uses a homotrimeric surface antigen to gain entry into host cells. Here we describe S-Trimer, native-like trimeric subunit vaccine candidate based on Trimer-Tag technology. Immunization of S-Trimer with either AS03 (oil-in-water emulsion) or CpG 1018 (TLR9 agonist) plus alum adjuvants induced high-level neutralizing antibodies and Th1-biased cellular immune responses in animal models....
Cytokine storm and multi-organ failure are the main causes of SARS-CoV-2-related death. However, origin excessive damages caused by SARS-CoV-2 remains largely unknown. Here we show that envelope (2-E) protein alone is able to cause acute respiratory distress syndrome (ARDS)-like in vitro vivo. 2-E proteins were found form a type pH-sensitive cation channels bilayer lipid membranes. As observed SARS-CoV-2-infected cells, heterologous expression induced rapid cell death various susceptible...
Safe, efficacious, and deployable vaccines are urgently needed to control COVID-19 in the large-scale vaccination campaigns. We report here preclinical studies of an approved protein subunit vaccine against COVID-19, ZF2001, which contains tandem-repeat dimeric receptor-binding domain (RBD) with alum-based adjuvant. assessed immunogenicity efficacy both mice non-human primates (NHPs). ZF2001 induced high levels RBD-binding SARS-CoV-2 neutralizing antibody primates, elicited balanced TH1/TH2...
Abstract Some people remain healthier throughout life than others but the underlying reasons are poorly understood. Here we hypothesize this advantage is attributable in part to optimal immune resilience (IR), defined as capacity preserve and/or rapidly restore functions that promote disease resistance (immunocompetence) and control inflammation infectious diseases well other causes of inflammatory stress. We gauge IR levels with two distinct peripheral blood metrics quantify balance between...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic. Antibody resistance dampens neutralizing antibody therapy and threatens current Coronavirus (COVID-19) vaccine campaigns. In addition to the emergence of resistant SARS-CoV-2 variants, little is known about how evades antibodies. Here, we report novel mechanism extracellular vesicle (EV)-mediated cell-to-cell transmission SARS-CoV-2, which facilitates escape from These EVs, initially observed in...
9α,13α-Dihydroxylisopropylidenylisatisine A (1), which was derived from isatisine (2) and possessed an unprecedented fused pentacyclic skeleton, isolated the leaves of Isatis indigotica Fort. The structure relative configuration were elucidated on basis extensive NMR analyses finally determined by single-crystal X-ray diffraction. Compound 1 showed moderate anti-HIV-1 activity with EC50 = 37.8 μM SI 7.98.
Peptidomics and genomics analyses were used to study an anti-infection array of peptides amphibian skin. 372 cDNA sequences antimicrobial characterized from a single individual skin the frog Odorrana grahami that encode 107 novel peptides. This contribution almost triples number currently reported The could be organized into 30 divergent groups, including 24 groups. diversity in peptide coding is, our knowledge, most extreme yet described for any animal. patterns diversification suggest...
Abstract COVID-19, caused by SARS-CoV-2, is an acute and rapidly developing pandemic, which leads to a global health crisis. SARS-CoV-2 primarily attacks human alveoli causes severe lung infection damage. To better understand the molecular basis of this disease, we sought characterize responses alveolar epithelium its adjacent microvascular endothelium viral under co-culture system. massive virus replication dramatic organelles remodeling in epithelial cells, alone. While, affected...
Long noncoding RNAs (lncRNAs) mediate important epigenetic regulation in a wide range of biological processes and diseases. We applied comprehensive analyses RNA-seq CAGE-seq (cap analysis gene expression sequencing) to characterize the dynamic changes lncRNA rhesus macaque ( Macaca mulatta ) brain four representative age groups. identified 18 anatomically diverse modules 14 mRNA representing spatial, age, sex specificities. Spatiotemporal- sex-biased were generally higher than those...
Abstract Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic worldwide. Currently, however, no effective drug or vaccine is available to treat prevent the resulting disease 2019 (COVID-19). Here, we report our discovery of promising anti-COVID-19 candidate, lipoglycopeptide antibiotic dalbavancin, based on virtual screening FDA-approved peptide library combined in vitro and vivo functional antiviral assays. Our results showed that dalbavancin...
Understanding the processes of immune regulation in patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial for improving treatment. Here, we performed longitudinal whole-transcriptome RNA sequencing on peripheral blood mononuclear cell (PBMC) samples from 18 disease 2019 (COVID-19) during their treatment, convalescence, and rehabilitation. After analyzing regulatory networks differentially expressed messenger RNAs (mRNAs), microRNAs (miRNAs) long...
Abstract SARS-CoV-2 infection-induced hyper-inflammation links to the acute lung injury and COVID-19 severity. Identifying primary mediators that initiate uncontrolled hypercytokinemia is essential for treatments. Mast cells (MCs) are strategically located at mucosa beneficially or detrimentally regulate immune inflammations. In this study, we showed SARS-CoV-2-triggered MC degranulation initiated alveolar epithelial inflammation injury. challenge induced in ACE-2 humanized mice rhesus...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global crisis, urgently necessitating the development of safe, efficacious, convenient-to-store, and low-cost vaccine options. A major challenge is that receptor-binding domain (RBD)-only fails to trigger long-lasting protective immunity if used alone for vaccination. To enhance antigen processing cross-presentation in draining lymph nodes (DLNs), we developed an interferon (IFN)-armed RBD dimerized by...
Abstract The emerging SARS-CoV-2 variants, commonly with many mutations in S1 subunit of spike (S) protein are weakening the efficacy current vaccines and antibody therapeutics. This calls for variant-proof targeting more conserved regions S protein. Here, we designed a recombinant vaccine, HR121, HR1 domain S2 HR121 consisting HR1–linker1–HR2–linker2–HR1, is conformationally functionally analogous to present fusion intermediate conformation subunit. Immunization rabbits rhesus macaques...
Objectives: There is conflicting data regarding the response of older people with HIV (PWH) to antiretroviral therapy (ART). The objective this study was evaluate long-term immunological and virological responses, changes in regimen, adverse drug reactions (ADRs) participants (50+ years) compared younger (18–34 middle-aged (35–49 PWH. Methods: A retrospective review medical records conducted on 1622 who received ART Yunnan Province, China, from 2010 2019. CD4 + T-cell counts, /CD8 ratio,...