A5021065130- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pulmonary Hypertension Research and Treatments
- Neonatal Respiratory Health Research
- MicroRNA in disease regulation
- Medical Imaging and Pathology Studies
- Liver physiology and pathology
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Prostate Cancer Treatment and Research
- Peptidase Inhibition and Analysis
- Cancer-related molecular mechanisms research
- Occupational and environmental lung diseases
- RNA modifications and cancer
- Selenium in Biological Systems
- Endoplasmic Reticulum Stress and Disease
- Protein Tyrosine Phosphatases
- Circular RNAs in diseases
- Viral Infections and Immunology Research
- Pancreatic function and diabetes
- Ubiquitin and proteasome pathways
- Extracellular vesicles in disease
- Biosensors and Analytical Detection
- COVID-19 Clinical Research Studies
- RNA Research and Splicing
- Inhalation and Respiratory Drug Delivery
- Autophagy in Disease and Therapy
Henan Normal University
2018-2025
Stony Brook University
2025
Institute of Molecular and Cell Biology
2023-2024
Yale University
2014-2023
Xinxiang University
2020
University of Pittsburgh
2005-2014
University of Pittsburgh Medical Center
2011-2013
Abstract Over the last decade, great enthusiasm has evolved for microRNA (miRNA) therapeutics. Part of excitement stems from fact that a miRNA often regulates numerous related mRNA s. As such, modulation single allows parallel regulation multiple genes involved in particular disease. While many studies have shown therapeutic efficacy using inhibitors, efforts to restore or increase function been lagging behind. The miR‐29 family gained lot attention its clear tissue fibrosis. This...
A large-scale effort, termed the Secreted Protein Discovery Initiative (SPDI), was undertaken to identify novel secreted and transmembrane proteins. In first of several approaches, a biological signal sequence trap in yeast cells utilized cDNA clones encoding putative second strategy various algorithms that recognize features such as hydrophobic properties sequences proteins encoded by expressed tags (ESTs) from human libraries. third approach surveyed ESTs for protein similarity set known...
Abstract Glutathione peroxidase 3 is a selenium-dependent enzyme playing critical role in detoxifying reactive oxidative species and maintaining the genetic integrity of mammalian cells. In this report, we found that expression glutathione (GPx3) was widely inactivated prostate cancers. Complete inactivation GPx3 correlates with poor clinical outcome. Deletions (hemizygous homozygous) gene are frequent cancer samples, occurring 39% samples studied. The rate methylation exon 1 region reaches...
Idiopathic Pulmonary Fibrosis (IPF) is characterized by profound changes in the lung phenotype including excessive extracellular matrix deposition, myofibroblast foci, alveolar epithelial cell hyperplasia and extensive remodeling. The role of epigenetic determining IPF unknown. In this study we determine whether lungs exhibit an altered global methylation profile.
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Significance Mismatched seasonal influenza vaccines are low efficacy and provide limited protection against circulating strains. A universal vaccine that can induce broadly cross-protective immunity is urgently needed. Here, we have developed a layered peptide-only nanoparticle delivered by dissolvable microneedle patches. Immunizations with the candidates elicited robust long-lasting protective immune responses in mice. We found unique vivo biodistribution feature of nanoparticles...
Abstract SARS-CoV-2 infection-induced hyper-inflammation links to the acute lung injury and COVID-19 severity. Identifying primary mediators that initiate uncontrolled hypercytokinemia is essential for treatments. Mast cells (MCs) are strategically located at mucosa beneficially or detrimentally regulate immune inflammations. In this study, we showed SARS-CoV-2-triggered MC degranulation initiated alveolar epithelial inflammation injury. challenge induced in ACE-2 humanized mice rhesus...
Viral evolution impacts diagnostic test performance through the emergence of variants with sequences affecting efficiency primer binding. Such that evade detection by nucleic acid-based tests are subject to selective pressure, enabling them spread more efficiently. Here, we report a variant-tolerant for SARS-CoV-2 using loop-mediated isothermal amplification (LAMP) assay containing high-fidelity DNA polymerase and polymerase-medicated probe (HFman probe). In addition demonstrating high...
Abstract Background Matrix metalloproteinases (MMPs) play important roles in remodeling the extracellular matrix and pathogenesis of idiopathic pulmonary fibrosis (IPF). MMP19, which is an MMP, was significantly upregulated hyperplastic alveolar epithelial cells IPF lung tissues promoted epithelial-mesenchymal transition (EMT). Recent studies have demonstrated that endothelial-to-mesenchymal (E(nd)MT) contributes to fibrosis. However, role MMP19 vascular injury repair E(nd)MT remains...
Abstract Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, fatal, and aging-associated interstitial lung disease with poor prognosis limited treatment options, while the pathogenesis remains elusive. In this study, we found that expression of nuclear receptor subfamily 2 group F member (NR2F2), steroid thyroid hormone superfamily receptors, was reduced in both IPF bleomycin-induced fibrotic lungs, markedly senescent epithelial cells. Inhibition NR2F2 increased senescence markers...
Abstract Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, fibrotic interstitial lung diseases, which mainly existed in middle-aged and elderly people. The accumulation of reactive oxygen species (ROS) common characteristic IPF. Previous research also shown that lactate levels can be abnormally elevated IPF patients. Emerging evidence suggested relationship between ROS needs further elucidation. In this article, we utilized mouse model BLM-induced to detect alterations other...
Abstract Idiopathic pulmonary fibrosis (IPF) is a chronic progressive disease with an abnormal accumulation of fibrotic tissue in the lung parenchyma and elevated glycolysis level associated cells without effective therapy options. Lactate significant factor aggravating IPF development, but main mechanism regulating needs further investigation. In this study, model was induced by bleomycin (BLM) intratracheally female C57BL/6 mice. The changes lactate markers were detected. For vitro...
Idiopathic pulmonary fibrosis (IPF) is an interstitial lung disease characterized by severe, progressive fibrosis. Roles for inflammation and oxidative stress have recently been demonstrated, but despite advances in understanding the pathogenesis, there are still no effective therapies IPF. This study investigates how extracellular superoxide dismutase (EC-SOD), a syndecan-binding antioxidant enzyme, inhibits We hypothesize that EC-SOD protects from oxidant damage preventing syndecan...
Rationale: Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by epithelial phenotypic changes and fibroblast activation. Based on the temporal heterogeneity of IPF, we hypothesized that hyperplastic alveolar cells regulate fibrotic response.Objectives: To identify novel mediators comparing transcriptional signature conserved in same lung.Methods: Laser capture microscope microarrays analysis were used to differentially expressed genes IPF lungs. Bleomycin-induced...
MicroRNAs are small noncoding RNAs that inhibit protein expression. We have previously shown the inhibition of microRNA let-7d in epithelial cells caused changes consistent with epithelial-to-mesenchymal transition (EMT) both vitro and vivo. The aim this study was to determine whether introduction into fibroblasts alters their mesenchymal properties. Transfection primary a decrease expression markers α-smooth muscle actin, N-cadherin, fibroblast-specific protein-1, fibronectin, as well an...
Abstract Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has a global pandemics. To facilitate the detection of infection, various RT-LAMP assays using 19 sets primers had been developed, but never compared. We performed comparative evaluation 4 RNA standards and 29 clinical samples from COVID-19 patients. Six 15 were firstly identified to have faster amplification when tested with four standards, further subjected parallel comparison remaining primer samples. Among these 10 sets,...
The heterogeneity of idiopathic pulmonary fibrosis (IPF) limits its diagnosis and treatment. association between the pathophysiological features serum protein signatures IPF currently remains unclear. present study analyzed specific proteins patterns associated with clinical parameters based on a proteomic dataset by data-independent acquisition using MS. Differentiated in sera distinguished patients into three subgroups signal pathways overall survival. Aging-associated weighted gene...
Idiopathic pulmonary fibrosis (IPF) is a progressive fatal interstitial lung disease without an effective cure. Herein, we explore the role of 3,5,3′-triiodothyronine (T3) administration on alveolar regeneration and at single-cell level. T3 supplementation significantly altered gene expression in fibrotic tissues. Immune cells were rapidly recruited into after injury; there much more M2 macrophages than M1 lungs bleomycin-treated mice; increased slightly, whereas reduced treatment. enhanced...