A5003537159- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Click Chemistry and Applications
- Receptor Mechanisms and Signaling
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Chemical Synthesis and Analysis
- Peptidase Inhibition and Analysis
- Ion channel regulation and function
- Lymphoma Diagnosis and Treatment
- Radiopharmaceutical Chemistry and Applications
- Lipid Membrane Structure and Behavior
- Heart Failure Treatment and Management
- Occupational Health and Performance
- Mass Spectrometry Techniques and Applications
- Pregnancy-related medical research
- Cholinesterase and Neurodegenerative Diseases
- Growth Hormone and Insulin-like Growth Factors
- Nicotinic Acetylcholine Receptors Study
- Photoreceptor and optogenetics research
- Analytical Chemistry and Chromatography
- Neuroendocrine Tumor Research Advances
- Cell Adhesion Molecules Research
- Advanced biosensing and bioanalysis techniques
- Toxin Mechanisms and Immunotoxins
Genentech
1992-2015
PDL BioPharma (United States)
2004-2010
Molecular Oncology (United States)
1998
Beth Israel Deaconess Hospital
1994
Harvard University
1994
Cornell University
1994
Tel Aviv University
1991
University of California System
1991
University at Buffalo, State University of New York
1987-1988
University of Chicago
1988
The proto-oncogene designated erb B2 or HER2 encodes a 185-kilodalton transmembrane tyrosine kinase (p185 ), whose overexpression has been correlated with poor prognosis in several human malignancies. A 45-kilodalton protein heregulin-α (HRG-α) that specifically induced phosphorylation of p185 was purified from the conditioned medium breast tumor cell line. Several complementary DNA clones encoding related HRGs were identified, all which are similar to proteins epidermal growth factor...
The heregulin/neu differentiation factor gene products were purified and cloned based on their ability to stimulate the phosphorylation of a 185-kDa protein in human breast carcinoma cell lines known express erbB2. However, not all cells that erbB2 respond heregulin, indicating other components besides may be required for heregulin binding. Cells are transfected with closely related receptor, erbB3, display single class lower affinity binding sites than has been previously observed lines....
Hormonal control of metabolic rate can be important in regulating the imbalance between energy intake and expenditure that underlies development obesity. In mice fed a high-fat diet, human fibroblast growth factor 19 (FGF19) increased [1.53 +/- 0.06 liters O(2)/h.kg(0.75) (vehicle) vs. 1.93 0.05 (FGF19); P < 0.001] decreased respiratory quotient [0.82 0.01 0.80 0.05]. contrast to vehicle-treated gained weight (0.14 g/mouse.d), FGF19-treated lost (-0.13 0.03 g/mouse.d; 0.001) without...
We report identification of interleukin (IL)-17E, a novel member the IL-17 family cytokines. IL-17E is ligand for recently identified protein termed EVI27/IL-17BR, which we term receptor homolog 1 (IL-17Rh1) in light multiple reported ligand-receptor relationships. Murine EVI27 was through its location at common site retroviral integration BXH2 murine myeloid leukemias. IL-17Rh1 shows highest level expression kidney with moderate other organs, whereas mRNA detected very low levels several...
The effects of recombinantly produced ob protein were compared to those food restriction in normal lean and genetically obese mice. Ob infusion into ob/ob mice resulted large decreases body fat-depot weight intake that persisted throughout the study. Smaller weights observed vehicle-treated fed same amount as consumed by protein-treated (pair feeding). In mice, significantly decreased weights, while decreasing a much lesser extent than Pair feeding did not reduce weights. potent weight-,...
Antibody drug conjugates (ADCs) combine the ideal properties of both antibodies and cytotoxic drugs by targeting potent to antigen-expressing tumor cells, thereby enhancing their antitumor activity. Successful ADC development for a given target antigen depends on optimization antibody selection, linker stability, potency, mode linker-drug conjugation antibody. Here, we systematically examined in vitro potency as well vivo preclinical efficacy safety profiles heterogeneous preparation...
ErbB3 is a member of the epidermal growth factor (EGF) receptor subfamily tyrosine kinases and believed to be for an unknown ligand. We have tested possibility that heregulin, possessing EGF-like domain, ligand ErbB3. found iodinated recombinant domain heregulin-beta 1 (125I-rHRG beta 1(177-244) bound specifically insect cell-expressed bovine with dissociation constant 0.85 nM. Moreover, 125I-rHRG NIH3T3 fibroblasts stably transfected erbB3 60 pM, but did not bind parental cells. could...
A large-scale effort, termed the Secreted Protein Discovery Initiative (SPDI), was undertaken to identify novel secreted and transmembrane proteins. In first of several approaches, a biological signal sequence trap in yeast cells utilized cDNA clones encoding putative second strategy various algorithms that recognize features such as hydrophobic properties sequences proteins encoded by expressed tags (ESTs) from human libraries. third approach surveyed ESTs for protein similarity set known...
Membranes from rabbit aorta and rat kidney cortex possess high-affinity (Kd = 10(-10) M) specific binding sites for atrial natriuretic factor (ANF). Similar are present in an established cell line pig kidney, LLC-PK1. Results of fractionation studies indicate that the receptors localized plasma membrane these tissues. The is time-dependent saturable. An excellent quantitative correlation was found between affinity synthetic ANF analogs intermediate activity to membranes half-maximal...
Antibody-drug conjugates (ADC), potent cytotoxic drugs covalently linked to antibodies via chemical linkers, provide a means increase the effectiveness of chemotherapy by targeting drug neoplastic cells while reducing side effects. Here, we systematically examine potential targets and linker-drug combinations that could an optimal ADC for treatment non-Hodgkin's lymphoma. We identified seven antigens (CD19, CD20, CD21, CD22, CD72, CD79b, CD180) lymphoma with ADCs. ADCs cleavable linkers...
Anti-cytomegalovirus (anti-CMV) hyperimmune globulin (HIG) has demonstrated efficacy in preventing CMV disease solid-organ transplant patients as well congenital when administered to pregnant women. To identify the neutralizing component of cytomegalovirus (CMV-HIG), we performed serial depletions CMV-HIG on cell-surface-expressed antigens purified antigens. Using this approach, demonstrate that major antibody response is directed at gH/gL/UL128/UL130/UL131 complex, suggesting little role...
ADVERTISEMENT RETURN TO ISSUEArticleNEXTStudies of the composition purified Torpedo californica acetylcholine receptor and its subunitsRichard L. Vandlen, Wilson C.-S. Wu, James C. Eisenach, Michael A. RafteryCite this: Biochemistry 1979, 18, 10, 1845–1854Publication Date (Print):May 1, 1979Publication History Published online1 May 2002Published inissue 1 1979https://pubs.acs.org/doi/10.1021/bi00577a001https://doi.org/10.1021/bi00577a001research-articleACS PublicationsRequest reuse...
Delivery of siRNA is a key hurdle to realizing the therapeutic promise RNAi. By targeting internalizing cell surface antigens, antibody–siRNA complexes provide possible solution. However, initial reports relied on non-specific charged interactions and have not been broadly applicable. To assess improve this delivery method, we built an industrial platform antibodies called THIOMABs, engineered enable precise covalent coupling siRNAs. We report that such generates monomeric conjugates (ARCs)...
The initial observation of the specificity in vivo blockade nicotinic acetylcholine receptor (AChR) by neurotoxins from Bungarus multicinctus α-toxin (α-BGT) and similar toxins Naja species (Lee Chang 1966; Lee et al. 1967) afforded unique tools for characterization these receptors at biochemical level. Quantities AChR suitable such types study are available only electric tissues Electrophorus electricus, Torpedo Narcine species. In vitro studies carried out present time with two...