A5007804636- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Solid State Laser Technologies
- Photodynamic Therapy Research Studies
- Melanoma and MAPK Pathways
- Optical Coherence Tomography Applications
- Optical Imaging and Spectroscopy Techniques
- Veterinary Equine Medical Research
- Advanced Optical Sensing Technologies
- Advanced MRI Techniques and Applications
- Animal Behavior and Welfare Studies
- Wildlife Ecology and Conservation
- Hepatitis C virus research
- Advanced Fiber Laser Technologies
- Computational Drug Discovery Methods
- Advanced Biosensing Techniques and Applications
- Infection Control in Healthcare
- Synthesis and biological activity
- Renal Transplantation Outcomes and Treatments
- Infection Control and Ventilation
- Analytical Chemistry and Sensors
- Peptidase Inhibition and Analysis
- Laser Material Processing Techniques
- RNA Interference and Gene Delivery
- Spectroscopy and Laser Applications
Colorado State University
2022-2024
The Francis Crick Institute
2020
Angiogenesis Foundation
2006-2008
Imaging Center
2007
Genentech
2007
University of Arkansas at Fayetteville
2005
Imperial College London
1998-2003
University of Oxford
2002
Imperial Valley College
2001
University of California, Los Angeles
1998-2000
Association with albumin as a means to improve biodistribution and tumor deposition of Fab was investigated using AB.Fab4D5, bifunctional molecule derived from trastuzumab (HERCEPTIN) capable binding antigen HER2 (erbB2) simultaneously. AB.Fab4D5 compared trastuzumab-derived (Fab4D5) for the ability target tumors overexpressing in mouse mammary virus/HER2 allograft models. Biodistribution monitored intravital microscopy, histology, integrated single-photon emission computed...
The usual paradigm for developing kinase inhibitors in oncology is to use a high-affinity proof-of-concept inhibitor with acceptable metabolic properties key target validation experiments. This approach requires substantial medicinal chemistry and can be confounded by drug toxicity off-target activities of the test molecule. As better alternative, we have developed inducible short-hairpin RNA xenograft models examine vivo efficacy inhibiting oncogenic BRAF. Our results show that tumor...
A whole‐field time‐domain fluorescence lifetime imaging (FLIM) microscope with the capability to perform optical sectioning is described. The excitation source a mode‐locked Ti:Sapphire laser that regeneratively amplified and frequency doubled 415 nm. Time‐gated intensity images at increasing delays after are acquired using gated microchannel plate image intensifier combined an intensified CCD camera. By fitting single or multiple exponential decay each pixel in field of view time‐gated...
Abstract Recombinant human rhApo2L/TRAIL selectively stimulates apoptosis in various cancer cells through its receptors DR4 and DR5, is currently clinical trials. Preclinical studies have established antitumor activity of models epithelial cancers; however, efficacy non-Hodgkin lymphoma (NHL) not well studied. Of 7 NHL cell lines tested vitro, stimulated BJAB, Ramos RA1, DoHH-2 cells. Rituximab, a CD20 antibody used to treat certain types NHL, augmented rhApo2L/TRAIL-induced caspase...
This article describes a wide-field time-domain fluorescence lifetime imaging (FLIM) microscope with optical sectioning. The FLIM system utilizes time-gated image intensifier, minimum gate width of 85 ps, to achieve high temporal resolution decays induced by ultrashort laser pulses. Different configurations, using excitation pulses picojoule energy at 80 MHz repetition rate and nanojoule 10 kHz, are compared. instrument has dynamic range spanning from 100 ps tens μs is shown have...
Highlights•Dry Hydrogen Peroxide for environmental infection prevention and control•Staff-initiated COVID-19 outbreak in long-term care facility impacted by DHP•Fully automated strategies may benefit staff-constrained LTCFAbstractA staff-initiated of among residents a was notable differences attack rates between units the association with dry hydrogen peroxide deployment continuous microbial reduction. Dry had previously been shown to significantly reduce bioburden facility, but not...
Tumor heterogeneity complicates the quantification of tumor microvascular characteristics assessed by dynamic contrast-enhanced MRI (DCE-MRI). To address this issue a novel approach was developed that combines DCE-MRI with diffusion-based multispectral (MS) analysis to quantify specific tissue populations. Diffusion-based MS segmentation (feature space: apparent diffusion coefficient, T(2) and proton density) performed identify populations were determined for each class. The ability...
We describe a novel three-dimensional fluorescence lifetime imaging microscope that exploits structured illumination to achieve whole-field sectioned images with spatial resolution of few micrometers.
Recent evidence has been accumulated showing that chronic hemodialysis (HD) patients have a very high prevalence of antibodies to hepatitis C virus (HCV). In contrast, there is little information addressing the virological characteristics HCV infection in this population. <b>Aim:</b> To measure viral load and correlate with demographic, biochemical, clinical features large cohort HCV-infected on HD. <b>Methods:</b> 394 HD were tested by branched-DNA signal...
We report a novel whole-field three-dimensional fluorescence lifetime imaging microscope that incoporates multispectral to provide five-dimensional (5-D) microscopy. This instrument, which can acquire 5-D data set in less than minute, is based on potentially compact and inexpensive diode-pumped solid-state laser technology. demonstrate spectral discrimination as well optical sectioning minimize artifacts determination illustrate how improves the contrast of biological tissue.
Abstract Background Conditional expression vectors have become a valuable research tool to avoid artefacts that may result from traditional gene studies. However, most systems require multiple plasmids must be independently engineered into the target system, resulting in experimental delay and an increased potential for selection of cell subpopulation differs significantly parental line. We therefore developed pHUSH, inducible system allows regulated shRNA, miRNA or cDNA cassettes on single...
There are two species of free-roaming feral equids in North America: horses ( Equus caballus ) and donkeys or “burros” E . asinus ). Both were introduced as domestic animals to America the early 1500s currently inhabit rangelands across western United States, Canada, all continents except Antarctica. Despite their global distribution, little is known about fine scale spatial ecology. Contemporary research tools assess space use include positioning system (GPS) tracking collars, but older...
A whole-field fluorescence lifetime imaging system is presented which based, for the first time, on an all-solid-state diode-pumped oscillator/amplifier system. Fluorescence of unstained biological tissue in vitro using this instrument demonstrated contrast between different constituents.
Abstract. Wide-field fluorescence lifetime imaging with spectral resolution and optical sectioning has been performed to achieve five-dimensional microscopy. Spectral filtering shown have the potential provide functional information about biological tissue by simultaneously measuring spectral/lifetime signature of sample. The use multispectral separate cellular components spatially their different emission wavelengths also demonstrated thus reducing artefacts in calculated maps. instrument...
Environmental infection transmission is a perennial problem in long-term care facilities (LTCFs), exacerbated by shared living arrangements, residents with cognitive deficits, staffing shortages, and suboptimal cleaning disinfection. This study evaluates the impact of dry hydrogen peroxide (DHP), as supplement to manual decontamination, on bioburden within an LTCF neurobehavioral unit.