A5030855454- Advanced Fluorescence Microscopy Techniques
- Medical Imaging Techniques and Applications
- Advanced X-ray and CT Imaging
- Advanced Radiotherapy Techniques
- Photoacoustic and Ultrasonic Imaging
- Optical Imaging and Spectroscopy Techniques
- Advanced MRI Techniques and Applications
- Photodynamic Therapy Research Studies
- Radiation Dose and Imaging
- Advanced Biosensing Techniques and Applications
- Optical Coherence Tomography Applications
- Robotics and Sensor-Based Localization
- Monoclonal and Polyclonal Antibodies Research
- Near-Field Optical Microscopy
- Advanced X-ray Imaging Techniques
- Infrastructure Maintenance and Monitoring
- Cellular Mechanics and Interactions
- Photosynthetic Processes and Mechanisms
- Robot Manipulation and Learning
- Radiation Therapy and Dosimetry
- Nanoplatforms for cancer theranostics
- Photonic and Optical Devices
- Cellular transport and secretion
- Radiomics and Machine Learning in Medical Imaging
- Robotic Path Planning Algorithms
Science and Technology Facilities Council
2007-2022
Research Complex at Harwell
2011-2022
Rutherford Appleton Laboratory
2010-2019
Biotechnology and Biological Sciences Research Council
2018
UK Research and Innovation
2018
Centro Laser
2016
Central Laser Facility
2016
University of Bristol
2015
University of Cambridge
2015
Imperial College London
2000-2014
Growing patterned rectangular objects The growth of usually requires a template to aid the positioning multiple materials. Qiu et al. used seeded crystallizable block copolymer and homopolymer produce highly uniform structures (see Perspective by Ballauff). Chemical etching, or dissolution, uncross-linked regions produced perforated platelet micelles. sequential addition different blends cross-linking/dissolution strategies allowed formation well-defined hollow micelles, which can be...
A cell membrane can be considered a liquid-phase plane in which lipids and proteins theoretically are free to diffuse. Numerous reports, however, describe retarded diffusion of animal cells. This anomalous results from combination structuring factors including protein-protein interactions, cytoskeleton corralling, lipid organization into microdomains. In plant cells, plasma-membrane (PM) have been described as relatively immobile, but the control mechanisms that structure PM not studied....
An emerging theme in cell biology is that surface receptors need to be considered as part of supramolecular complexes proteins and lipids facilitating specific receptor conformations distinct distributions, e.g., at the immunological synapse. Thus, a new goal develop bioimaging not only locates live cells but can also probe their environment. Such technique demonstrated here using fluorescence lifetime imaging green fluorescent protein (GFP). We first show, by time-correlated single-photon...
A new method is proposed for scatter-correction of cone-beam CT images.A coarse reconstruction used in initial iteration steps.Modelling the x-ray tube spectra and detector response are included algorithm.Photon diffusion inside imaging subject calculated using Monte Carlo method.Photon scoring at forced detection to a fixed set node points.The scatter profiles then obtained by linear interpolation.The algorithm referred as Coarse Reconstruction Fixed Detection (CRFD) technique.Scatter...
Axons are cable-like neuronal processes wiring the nervous system. They contain parallel bundles of microtubules as structural backbones, surrounded by regularly spaced actin rings termed periodic membrane skeleton (PMS). Despite being an evolutionarily conserved, ubiquitous, highly ordered feature axons, function PMS is unknown. Here we studied abundance, organization, and function, combining versatile Drosophila genetics with superresolution microscopy various functional readouts. Analyses...
Plant plasma-membrane (PM) proteins are involved in several vital processes, such as detection of pathogens, solute transport, and cellular signaling. For these to function effectively there needs be structure within the PM allowing, for example, same signaling cascade spatially organized. Here we demonstrate that with divergent functions located clusters differing size membrane using subdiffraction-limited Airyscan confocal microscopy. Single particle tracking reveals move at different...
A whole‐field time‐domain fluorescence lifetime imaging (FLIM) microscope with the capability to perform optical sectioning is described. The excitation source a mode‐locked Ti:Sapphire laser that regeneratively amplified and frequency doubled 415 nm. Time‐gated intensity images at increasing delays after are acquired using gated microchannel plate image intensifier combined an intensified CCD camera. By fitting single or multiple exponential decay each pixel in field of view time‐gated...
We have developed a wide-field time-resolved imaging system to image quantitatively both the fluorescence lifetime and rotational correlation time of fluorophore. Using polarization-resolved imager, we simultaneously orthogonal polarization components emission onto time-gated intensified CCD. demonstrate solvent viscosity variations through fluorescein in multiwell plate apply this technique probe microviscosity live cells.
Integrins undergo global conformational changes that specify their activation state. Current models portray the inactive receptor in a bent conformation upon converts to fully extended form which integrin subunit leg regions are separated enable ligand binding and subsequent signaling. To test applicability of this model adherent cells, we used fluorescent resonance energy transfer (FRET)–based approach, combination with engineered mutants monoclonal antibody reporters, image α5β1...
We have applied fluorescence lifetime imaging (FLIM) to the autofluorescence of different kinds biological tissue in vitro, including animal sections and knee joints as well human teeth, obtaining two-dimensional maps with functional contrast. find that decay profiles are described by stretched exponential function (StrEF), which can represent complex nature tissue. The StrEF yields a continuous distribution lifetimes, be extracted an inverse Laplace transformation, additional information is...
Analytical methods that enable visualization of nanomaterials derived from solution self-assembly processes in organic solvents are highly desirable. Herein, we demonstrate the use stimulated emission depletion microscopy (STED) and single molecule localization (SMLM) to map living crystallization-driven block copolymer (BCP) media at sub-diffraction scale. Four different dyes were successfully used for single-colour super-resolution imaging BCP nanostructures allowing micelle length...
This article describes a wide-field time-domain fluorescence lifetime imaging (FLIM) microscope with optical sectioning. The FLIM system utilizes time-gated image intensifier, minimum gate width of 85 ps, to achieve high temporal resolution decays induced by ultrashort laser pulses. Different configurations, using excitation pulses picojoule energy at 80 MHz repetition rate and nanojoule 10 kHz, are compared. instrument has dynamic range spanning from 100 ps tens μs is shown have...
Total internal reflection fluorescence microscopy (TIRFM) has been proven to be an extremely powerful technique in animal cell research for generating high contrast images and dynamic protein conformation information. However, there long a perception that TIRFM is not feasible plant cells because the wall would restrict penetration of evanescent field lead scattering illumination. By comparative analysis epifluorescence TIRF root cells, it demonstrated can generate images, superior other...
Scatter in a detector and its housing can result image degradation. Typically, such scatter leads to low-spatial frequency ‘glare’ superimposed on the primary signal. We infer glare-spread function (GSF) of an amorphous-silicon flat-panel via edge-spread technique. demonstrate that this spread (referred as ‘scatter-glare’ herein) causes drop associated modulation-transfer function. This results compression range reconstructed CT (computed tomography) numbers is impediment accurate CT-number...
Abstract From biomineralization to synthesis, organic additives provide an effective means of controlling crystallization processes. There is growing evidence that these are often occluded within the crystal lattice. This promises elegant creating nanocomposites and tuning physical properties. Here we use incorporation sulfonated fluorescent dyes gain new understanding additive occlusion in calcite (CaCO 3 ), link morphological changes mechanisms. We demonstrate incorporated specific zones,...
We report a picosecond time-gated fluorescence lifetime imaging (FLIM) system extended to perform time-resolved anisotropy (TR-FAIM). Upon excitation with linearly polarized laser pulses, the parallel and perpendicular components of emission from sample are imaged simultaneously using polarization-resolved imager. The technique presented here quantitatively reports rotational mobility fluorophore as it varies according local environment. In single acquisition run yields maps both correlation...
We describe a novel three-dimensional fluorescence lifetime imaging microscope that exploits structured illumination to achieve whole-field sectioned images with spatial resolution of few micrometers.
The Rutherford Appleton Laboratory clinical positron camera consists of two opposed multiwire proportional chambers (MWPCs) mounted on a rotating gantry capable performing routine nuclear medicine studies. system has operated since the end 1986 with complete reliability. It sensitivity 37 kcps MBq-1 cm3 per axial cm for 20 diameter uniformly filled cylinder activity. best spatial resolution obtainable is about 6 mm, although in practice images are smoothed order to reduce statistical noise...
Several techniques for performing digital image restoration are reviewed and the problems associated with evaluating processing discussed. An application of constrained deconvulution to images liver produced by single-photon emission computed tomography is presented. Specific evaluation criteria suggested based on these, choice conditions best suited proposed. Typically cold tumor contrast can be improved a factor greater than 2 whilst mottle increases negligibly.