A5043985590- Nuclear Structure and Function
- Polysaccharides and Plant Cell Walls
- Genomics and Chromatin Dynamics
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- RNA Research and Splicing
- DNA Repair Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant and Biological Electrophysiology Studies
- Advanced Electron Microscopy Techniques and Applications
- Plant Molecular Biology Research
- Tree Root and Stability Studies
- Molecular Biology Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Plant Pathogenic Bacteria Studies
- Microtubule and mitosis dynamics
- Pickering emulsions and particle stabilization
- Plant Surface Properties and Treatments
- Pregnancy and preeclampsia studies
- Social Acceptance of Renewable Energy
- Cellular transport and secretion
University of Warwick
2021-2023
Ollscoil na Gaillimhe – University of Galway
2023
University of California, Berkeley
2023
Oxford Brookes University
2014-2020
Imperial College London
2011-2018
Max Planck Institute of Molecular Plant Physiology
2012
Norwich Research Park
2011
Sainsbury Laboratory
2011
Abstract The plant cell wall is a dynamic and complex structure whose functional integrity constantly being monitored maintained during development interactions with the environment. In response to damage (CWD), putatively compensatory responses, such as lignin production, are initiated. this context, deposition could reinforce maintain integrity. Lignin important for plant’s environmental stress, reinforcement secondary formation, long-distance water transport. Here, we identify two stages...
Plants actively perceive and respond to perturbations in their cell walls which arise during growth, biotic abiotic stresses. However, few components involved plant wall integrity sensing have been described date. Using a reverse-genetic approach, we identified the Arabidopsis thaliana leucine-rich repeat receptor kinase MIK2 as an important regulator of damage responses triggered upon cellulose biosynthesis inhibition. Indeed, loss-of-function mik2 alleles are strongly affected immune...
Cell wall integrity and immune signaling systems cooperatively mediate responses to cell damage in the model plant Arabidopsis .
Plant plasma-membrane (PM) proteins are involved in several vital processes, such as detection of pathogens, solute transport, and cellular signaling. For these to function effectively there needs be structure within the PM allowing, for example, same signaling cascade spatially organized. Here we demonstrate that with divergent functions located clusters differing size membrane using subdiffraction-limited Airyscan confocal microscopy. Single particle tracking reveals move at different...
Cellulose is the most abundant biopolymer in world, main load-bearing element plant cell walls, and represents a major sink for carbon fixed during photosynthesis. Previous work has shown that photosynthetic activity partially regulated by carbohydrate sinks. However, coordination of cellulose biosynthesis with metabolism photosynthesis not well understood. Here, we demonstrate inhibition (CBI) leads to reductions transcript levels genes involved photosynthesis, Calvin cycle, starch...
Plastids are multifunctional, pleomorphic organelles of purported endosymbiotic origin that in plants and green algae display a characteristic double membrane envelope ([Wise, 2007][1]). All plastids originate from colorless proplastids, simple pigmentation-based classification distinguishes
Seeds of dicotyledonous plants store proteins in dedicated membrane-bounded organelles called protein storage vacuoles (PSVs). Formed during seed development through morphological and functional reconfiguration lytic embryos [M. Feeney et al. , Plant Physiol. 177, 241–254 (2018)], PSVs undergo division the later stages maturation. Here, we study biophysical mechanism PSV morphogenesis vivo, discovering that micrometer-sized liquid droplets containing form within vacuolar lumen phase...
ABSTRACT The bacterial plant pathogen Pseudomonas syringae causes disease in a wide range of plants. associated decrease crop yields results economic losses and threatens global food security. Competition exists between the immune system pathogen, basic principles which can be applied to animal infection pathways. P. uses type III secretion (T3SS) deliver virulence factors into that promote survival bacterium. T3SS is product hypersensitive response pathogenicity ( hrp ) conserved hrc gene...
The linker of nucleoskeleton and cytoskeleton (LINC) complex is an essential multi-protein structure spanning the eukaryotic nuclear envelope. LINC functions to maintain architecture, positioning, mobility, along with specialized in meiotic prophase chromosome segregation. Members were recently identified maize, important scientific agricultural grass species. Here we characterized
The linker of nucleoskeleton to cytoskeleton (LINC) complex is an essential multi-protein structure spanning the nuclear envelope. It connects cytoplasm nucleoplasm, functions maintain shape and architecture regulates chromosome dynamics during cell division. Knowledge LINC composition function in plant kingdom primarily limited Arabidopsis, but critically missing from evolutionarily distant monocots, which include grasses, most important agronomic crops worldwide. To fill this knowledge...
In plant cells, molecular connections link the cell wall–plasma membrane–actin cytoskeleton to form a continuum. It is hypothesized that wall provides stable anchor points around which actin remodels. Here we use live imaging of fluorescently labelled marker proteins quantify organization and dynamics determine impact disrupting within Labelling with green fluorescent protein (GFP)–fimbrin actin-binding domain 2 (FABD2) resulted in network composed fine filaments thicker bundles appeared as...
Breast cancer is the most commonly occurring in women worldwide and second common overall. The development of new therapies to treat this devastating malignancy needed urgently. Nanoparticles are one class nanomaterial with multiple applications medicine, ranging from their use as drug delivery systems promotion changes cell morphology control gene transcription. made natural polymer chitosan easy produce, have a very low immunogenic profile, diffuse easily into cells. One hallmark feature...
In eukaryotes, the nuclear envelope (NE) encloses chromatin and separates it from rest of cell. The Linker Nucleoskeleton Cytoskeleton (LINC) complex physically bridges across NE, linking cytoplasmic components. plants, these LINC complexes are beginning to be ascribed roles in cellular functions, including organization, regulation nuclei shape movement, cell division. Homologs core components, KASH SUN proteins, have previously been identified maize. Here, we characterized presumed...
Genomic deoxyribonucleic acid (DNA) is continuously being damaged by endogenous processes such as metabolism or exogenous events radiation. The specific phosphorylation of histone H2AX on serine residue 139, described γ-H2AX, an excellent indicator marker DNA double-strand breaks (DSBs). yield γ-H2AX (foci) shown to have some correlation with the dose radiation other DSB-causing agents. However, there discrepancy in DSB foci among imaging and methods gel electrophoresis. Super-resolution...
Protein targeting to the inner nuclear membrane (INM) is one of least understood protein pathways. INM proteins are important for chromatin organization, morphology and movement, meiosis, have been implicated in human diseases. In opisthokonts, mechanism transport factor-mediated trafficking, which localization signals (NLSs) function import transmembrane proteins. To explore whether this pathway exists plants, we fused SV40 NLS a plant ER tail-anchored showed that GFP-tagged fusion was...
Purpose To combat climate change, protect biodiversity, maintain water quality, facilitate a just transition for workers and engage citizens communities, diversity of stakeholders across multiple levels work together collaborate to co-create mutually beneficial solutions. This paper aims illustrate how 7.5-year collaboration between local researchers, academics, companies, state agencies policymakers is contributing the reframing industrial harvested peatlands regenerative ecosystems carbon...
Abstract Plant plasma-membrane (PM) proteins are involved in several vital processes, such as detection of pathogens, solute transport and cellular signalling. Recent models suggest that for these to function effectively there needs be structure within the PM allowing, example, same signalling cascade spatially organized. Here we demonstrate with divergent functions located clusters differing size membrane using sub-diffraction-limited Airyscan confocal microscopy. In addition, single...
Abstract Plant cells are surrounded by walls, which must often meet opposing functional requirements during plant growth and defense. The them modifying wall structure composition in a tightly controlled adaptive manner. modifications seem to be mediated dedicated cell integrity (CWI) maintenance mechanism. Currently the mode of action mechanism is not understood it unclear how its activity coordinated with established defense signaling. We investigated responses induced damage (CWD)...
Biological sample preparation is a major challenge for any type of electron microscopy.Energy dispersive X-ray spectrometry (EDS) detects the characteristic X-rays produced as result specimenbeam interactions in an microscope and identifies elements present sample.Scanning microscopy-EDS (SEM-EDS) typically applied to hard materials using relatively high accelerating voltages beam currents produce accurate quantitative data about composition.The application SEM-EDS biological samples has...
Abstract The plant secretory pathway is responsible for the production of majority proteins and carbohydrates consumed on planet. early composed Golgi bodies endoplasmic reticulum (ER) highly mobile in plants with rapid remodelling ER network. dynamics driven by actin cytoskeleton myosin motor play a key role this. However, exactly how drive currently contentious issue. Here, using combination live cell microscopy over-expression non-functional myosins we demonstrate that filament sliding...
ABSTRACT In eukaryotes, the nuclear envelope (NE) encloses chromatin and separates it from rest of cell. The Linker Nucleoskeleton Cytoskeleton (LINC) complex physically bridges across NE, linking cytoplasmic components. plants, these LINC complexes are beginning to be ascribed roles in cellular functions, including organization, regulation nuclei shape movement, cell division. Homologs core components, KASH SUN proteins, have previously been identified maize. Here, we characterized presumed...