A5076713300- Plant Reproductive Biology
- Plant Molecular Biology Research
- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Microtubule and mitosis dynamics
- Lipid Membrane Structure and Behavior
- Biochemical and Structural Characterization
- Polysaccharides and Plant Cell Walls
- Biotin and Related Studies
- Glycosylation and Glycoproteins Research
- Plant Stress Responses and Tolerance
- Ubiquitin and proteasome pathways
- Plant Gene Expression Analysis
- Legume Nitrogen Fixing Symbiosis
- RNA Research and Splicing
- Phytoplasmas and Hemiptera pathogens
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Mycorrhizal Fungi and Plant Interactions
- Genomics and Chromatin Dynamics
- Endoplasmic Reticulum Stress and Disease
- Chromosomal and Genetic Variations
Ghent University
2016-2025
VIB-UGent Center for Plant Systems Biology
2016-2025
Virogenomics BioDevelopment (United States)
2024
VIB-UGent Center for Medical Biotechnology
2024
Ghent University Hospital
2022
Vlaams Instituut voor Biotechnologie
2006-2019
Center for Systems Biology
2008
University of Tübingen
2008
University of Edinburgh
2008
Duke University
2008
During the development of multicellular organisms, organogenesis and pattern formation depend on formative divisions to specify maintain pools stem cells. In higher plants, these activities are essential shape final root architecture because functioning apical meristems de novo lateral roots entirely rely it. We used transcript profiling sorted pericycle cells undergoing initiation identify receptor-like kinase ACR4 Arabidopsis as a key factor both in promoting cell constraining number once...
In Arabidopsis , lateral root primordia (LRPs) originate from pericycle cells located deep within the parental and have to emerge through endodermal, cortical, epidermal tissues. These overlaying tissues place biomechanical constraints on LRPs that are likely impact their morphogenesis. This study probes interplay between patterns of cell division, organ shape, LRP morphogenesis by exploiting recent advances in live plant imaging image analysis. Our 3D/4D analysis revealed early stage...
The plant root cap, surrounding the very tip of growing root, perceives and transmits environmental signals to inner tissues. In Arabidopsis thaliana, auxin released by cap contributes regular spacing lateral organs along primary axis. Here, we show that periodicity organ induction is driven recurrent programmed cell death at most distal edge cap. We suggest synchronous bursts in cells release pulses tissues, establishing pattern for formation. dynamics turnover may therefore coordinate...
To identify molecular players implicated in cytokinesis and division plane determination, the Arabidopsis thaliana genome was explored for potential genes. More than 100 open reading frames were selected based on similarity to yeast animal genes, cytoskeleton polarity Nicotiana tabacum genes showing cell cycle-controlled expression. The subcellular localization of these proteins determined by means GFP tagging tobacco Bright Yellow-2 cells plants. Detailed confocal microscopy identified 15...
Abstract Clathrin-mediated endocytosis (CME) regulates many aspects of plant development, including hormone signaling and responses to environmental stresses. Despite the importance this process, machinery that CME in plants is largely unknown. In mammals, heterotetrameric ADAPTOR PROTEIN COMPLEX-2 (AP-2) required for formation clathrin-coated vesicles at plasma membrane (PM). Although existence AP-2 has been predicted Arabidopsis thaliana, biochemistry functionality complex still...
Endomembrane trafficking relies on the coordination of a highly complex, dynamic network intracellular vesicles. Understanding will require dissection cargo and vesicle dynamics at cellular level in vivo. This is also key to establishing link between vesicular networks their functional roles development. We used high-content screen discover small molecules targeting endomembrane vivo complex eukaryote, Arabidopsis thaliana. Tens thousands were prescreened selected subset was interrogated...
Plant cytokinesis deploys a transport system that centers cell plate-forming vesicles and fuses them to form plate. Here we show the adaptin-like protein TPLATE clathrin light chain 2 (CLC2) are targeted expanding plate equatorial subregion of plasma membrane referred as cortical division zone (CDZ). Bimolecular fluorescence complementation immunodetection indicates interacts with clathrin. Pharmacological tools well analysis targeting in mutant background affecting formation allowed...
Article6 August 2016Open Access Transparent process The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis Annika K Weimer Department of Molecular Mechanisms Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du CNRS, IBMP-CNRS UPR2357, Université Strasbourg, Strasbourg Cedex, France Search for more papers by this author Sascha Biedermann Hirofumi Harashima orcid.org/0000-0003-3370-4111 RIKEN Center Sustainable Resource Science,...
Proximity labeling is a powerful approach for detecting protein-protein interactions. Most proximity techniques use promiscuous biotin ligase or peroxidase fused to protein of interest, enabling the covalent proteins and subsequent capture identification interacting neighboring without need complex remain intact. To date, only few studies have reported on in plants. Here, we present results systematic study applying variety biotin-based approaches several plant systems using various...
The preprophase band (PPB) is a faithful but transient predictor of the division plane in somatic cell divisions. Throughout mitosis PPBs positional information preserved by factors that continuously mark at cortex, cortical zone, their distinct spatio-temporal localization patterns. However, mechanism maintaining these identity plasma membrane after PPB disassembly remains obscure. pair kinesin-12 class proteins PHRAGMOPLAST ORIENTING KINESIN1 (POK1) and POK2 are key players maintenance....
Abstract The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both homologues yeast ARP2/3 activator, Pan1p. Here, we show that these also involved in actin cytoskeleton regulated autophagy. AtEH/Pan1 localise to plasma membrane autophagosomes. Upon induction autophagy, recruit TPC AP-2 subunits, clathrin, Increased expression boosts autophagosome formation, suggesting independent redundant pathways...
Abstract ATP production requires the establishment of an electrochemical proton gradient across inner mitochondrial membrane. Mitochondrial uncouplers dissipate this and disrupt numerous cellular processes, including vesicular trafficking, mainly through energy depletion. Here we show that Endosidin9 (ES9), a novel uncoupler, is potent inhibitor clathrin-mediated endocytosis (CME) in different systems ES9 induces inhibition CME not because its effect on ATP, but rather due to protonophore...
To sustain a lifelong ability to initiate organs, plants retain pools of undifferentiated cells with preserved proliferation capacity. The root pericycle represents unique tissue conditional meristematic activity, and its tight control determines initiation lateral organs. Here we show that the activity is constrained by interaction adjacent endodermis. Release these restraints elimination endodermal single-cell ablation triggers re-enter cell cycle. We found endodermis removal substitutes...
Abstract Endoplasmic reticulum–plasma membrane contact sites (ER–PM CS) play fundamental roles in all eukaryotic cells. Arabidopsis thaliana mutants lacking the ER–PM protein tether synaptotagmin1 (SYT1) exhibit decreased PM integrity under multiple abiotic stresses, such as freezing, high salt, osmotic stress, and mechanical damage. Here, we show that, together with SYT1, stress-induced SYT3 is an that also functions maintaining integrity. The CS localization of SYT1 dependent on...
In eukaryotes, clathrin-coated vesicles (CCVs) facilitate the internalization of material from cell surface as well movement cargo in post-Golgi trafficking pathways. This diversity functions is partially provided by multiple monomeric and multimeric clathrin adaptor complexes that provide compartment selectivity. The adaptor-protein assembly polypeptide-1 (AP-1) complex operates part secretory pathway at trans-Golgi network (TGN), while AP-2 TPLATE jointly operate plasma membrane to execute...
Plant cells produce different microtubule arrays that are essential for cell division and morphogenesis without equivalent in other eukaryotes. Microtubule-associated proteins influence the behavior of microtubules is presumed to culminate into transitions from one array another. We analyzed microtubule-binding properties three Arabidopsis (Arabidopsis thaliana) members, AtMAP65-1, AtMAP65-4, AtMAP65-5, live using laser scanning confocal microscopy. Depending on overall organization cortical...
Aurora-like kinases play key roles in chromosome segregation and cytokinesis yeast, plant, animal systems. Here, we characterize three Arabidopsis thaliana protein kinases, designated AtAurora1, AtAurora2, AtAurora3, which share high amino acid identities with the Ser/Thr kinase domain of yeast Ipl1 Auroras. Structure expression AtAurora1 AtAurora2 suggest that these genes arose by a recent gene duplication, whereas diversification plant alpha beta Aurora predates origin land plants. The...