A5064886500- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Transgenic Plants and Applications
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Plant Genetic and Mutation Studies
- Genomics and Chromatin Dynamics
- Plant-Microbe Interactions and Immunity
- bioluminescence and chemiluminescence research
- Plant Gene Expression Analysis
- Polysaccharides and Plant Cell Walls
- Chromatin Remodeling and Cancer
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Light effects on plants
- Legume Nitrogen Fixing Symbiosis
- Insect Resistance and Genetics
- Mechanisms of cancer metastasis
- Cancer Mechanisms and Therapy
- Polyamine Metabolism and Applications
- Plant Virus Research Studies
Max Planck Institute for Plant Breeding Research
2012-2022
HUN-REN Szegedi Biológiai Kutatóközpont
2012-2022
Institute of Plant Biology
2012-2022
Hungarian Academy of Sciences
2010-2022
Max Planck Society
2005-2014
Budapest University of Technology and Economics
2008
Universitat de Barcelona
2003-2008
Centro de Investigaciones Biológicas Margarita Salas
2008
Universitat de València
2008
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2008
Summary Δ‐1‐pyrroline‐5‐carboxylate synthetase enzymes, which catalyse the rate‐limiting step of proline biosynthesis, are encoded by two closely related P5CS genes in Arabidopsis. Transcription is differentially regulated drought, salinity and abscisic acid, suggesting that these play specific roles control biosynthesis. Here we describe genetic characterization p5cs insertion mutants, indicates P5CS1 required for accumulation under osmotic stress. Knockout mutations result reduction...
An insertion element [transferred DNA (T-DNA)], transferred by soil agrobacteria into the nuclear genome of plants, was used for induction gene fusions in Arabidopsis thaliana, Nicotiana tabacum, and plumbaginifolia. A promoterless aph(3')II (aminoglycoside phosphotransferase II) reporter linked to right end T-DNA transformed plants along with a plasmid replicon selectable hygromycin-resistance gene. Transcriptional translational were identified screening APH(3')II enzyme activity diverse...
Summary Proline is a common compatible osmolyte in higher plants. accumulation response to water stress and salinity preceded by rapid increase of the mRNA level Δ 1 ‐pyrroline‐5‐carboxylate synthase (P5CS) controlling rate‐limiting step glutamate‐derived proline biosynthesis. P5CS encoded two differentially regulated genes Arabidopsis . Gene AtP5CS1 mapped chromosome 2‐78.5 expressed most plant organs, but silent dividing cells. AtP5CS2 located close marker m457 on 3–101.3 contributes...
The levels of endogenous polyamines have been shown to increase in plant cells challenged with low temperature; however, the functions regulation cold stress responses are unknown. Here, we show that accumulation putrescine under is essential for proper acclimation and survival at freezing temperatures because Arabidopsis (Arabidopsis thaliana) mutants defective biosynthesis (adc1, adc2) display reduced tolerance compared wild-type plants. Genes ADC1 ADC2 different transcriptional profiles...
Abstract Heat shock factors (HSFs) are principal regulators of plant responses to several abiotic stresses. Here, we show that estradiol-dependent induction HSFA4A confers enhanced tolerance salt and oxidative agents, whereas inactivation results in hypersensitivity stress Arabidopsis (Arabidopsis thaliana). Estradiol transgenic plants decreases, while the knockout hsfa4a mutation elevates hydrogen peroxide accumulation lipid peroxidation. Overexpression alters transcription a large set...
The ethylene response factor VII (ERF-VII) transcription RELATED TO APETALA2.12 (RAP2.12) was previously identified as an activator of the ALCOHOL DEHYDROGENASE1 promoter::luciferase (ADH1-LUC) reporter gene. Here we show that overexpression RAP2.12 and its homologues RAP2.2 RAP2.3 sustains ABA-mediated activation ADH1 activates hypoxia marker genes under both anoxic normoxic conditions. Inducible expression all three RAP2s conferred tolerance to anoxia, oxidative osmotic stresses, enhanced...
Summary Brassinosteroids which show high structural similarity to animal steroid hormones elicit a variety of growth responses when exogeneously applied plant tissues. Thus far however, the function endogeneous brassinosteroids in higher plants has been unclear. This paper describes three extremely dwarfed Arabidopsis thaliana mutants, cbb1 ( dwf1‐6 ), cbb2 and cbb3 , are impared cell elongation controlled by brassinosteroids. While ) can be phenotypically normalized wild‐type feeding with...
Light signaling via the phytochrome A (phyA) photoreceptor controls basic plant developmental processes including de-etiolation and hypocotyl elongation. We have identified a new Arabidopsis mutant, pat (phytochrome signal transduction)1-1, which shows strongly reduced responses in continuous far-red light. Physiological molecular data indicate that this mutant is disrupted at an early step of phyA transduction. The PAT1 gene encodes cytoplasmic protein 490 amino acids with sequence...
The prl1 mutation localized by T-DNA tagging on Arabidopsis chromosome 4-44 confers hypersensitivity to glucose and sucrose. results in transcriptional derepression of responsive genes defining a novel suppressor function signaling. also augments the sensitivity plants growth hormones including cytokinin, ethylene, abscisic acid, auxin; stimulates accumulation sugars starch leaves; inhibits root elongation. PRL1 encodes regulatory WD protein that interacts with ATHKAP2, an α-importin nuclear...
Null-mutations of the Arabidopsis FKBP-like immunophilin TWISTED DWARF1 (TWD1) gene cause a pleiotropic phenotype characterized by reduction cell elongation and disorientated growth all plant organs. Heterologously expressed TWD1 does not exhibit cis-trans-peptidylprolyl isomerase (PPIase) activity complement yeast FKBP12 mutants, suggesting that acts indirectly via protein-protein interaction. Yeast two-hybrid protein interaction screens with identified cDNA sequences encode C-terminal...
In an attempt to increase the insecticidal effect of delta-endotoxin crystal protein CryIC on relatively Cry-insensitive larvae Spodoptera littoralis, a combination and endochitinase was used. comprising first 756 amino acids from Bacillus thuringiensis K26-21 ChiAII encoded by Serratia marcescens were separately produced in Escherichia coli carrying genes overexpression vectors. The its own, even at very low concentrations (0.1 microgram/ml), perforated larval midgut peritrophic membrane....