A5060543785- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- Genetic Syndromes and Imprinting
- Seed Germination and Physiology
- Plant Gene Expression Analysis
- Plant responses to water stress
- Plant Taxonomy and Phylogenetics
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Berry genetics and cultivation research
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Chromatin Dynamics
- Redox biology and oxidative stress
- Microtubule and mitosis dynamics
- Bee Products Chemical Analysis
- Bayesian Methods and Mixture Models
- Enzyme Structure and Function
- Statistical Methods and Bayesian Inference
- MicroRNA in disease regulation
- Heat shock proteins research
University of Oslo
2012-2024
Institut thématique Génétique, génomique et bioinformatique
2023
Norwegian University of Science and Technology
2011-2012
UiT The Arctic University of Norway
2006
University of Tübingen
1998-2002
Praxis für Humangenetik Tübingen
1996-1997
Polycomb repressive complex 2 (PRC2) is a key regulator of epigenetic states catalyzing histone H3 lysine 27 trimethylation (H3K27me3), chromatin mark. PRC2 composition conserved from humans to plants, but the function during early stage plant life unclear beyond fact that it required for development endosperm, nutritive tissue supports embryo growth. Circumventing requirement in endosperm allowed us generate viable homozygous null mutants FERTILIZATION INDEPENDENT ENDOSPERM (FIE), which...
Abstract Background MADS-domain transcription factors play important roles during plant development. The Arabidopsis MADS-box gene SHORT VEGETATIVE PHASE ( SVP ) is a key regulator of two developmental phases. It functions as repressor the floral transition vegetative phase and later it contributes to specification meristems. How these distinct activities are conferred by single factor unclear, but interactions with other MADS domain proteins which specify binding different genomic regions...
Significance Hybrid seed lethality has been recognized and addressed by a long-standing tradition of plant-breeding research. Nevertheless, its role in evolution speciation underestimated. In this study, hybrid between Arabidopsis lyrata arenosa , two model species growing interest the scientific community, was investigated. This study shows that endosperm defects are sufficient to explain direction gene flow wild species, suggesting an important hybridization barrier plant speciation....
Background SET-domain proteins are histone lysine (K) methyltransferases (HMTase) implicated in defining transcriptionally permissive or repressive chromatin. The Arabidopsis ASH1 HOMOLOG 2 (ASHH2) protein (also called SDG8, EFS and CCR1) has been suggested to methylate H3K4 and/or H3K36 is similar Drosophila ASH1, a positive maintainer of gene expression, yeast Set2, HMTase. Mutation the ASHH2 pleiotropic developmental effects. Here we focus on role plant reproduction. Methodology/Principal...
Abstract As in other eukaryotes, progression through the cell cycle plants is governed by cyclin-dependent kinases. Phosphorylation of a canonical Thr residue T-loop kinases required for high enzyme activity animals and yeast. We show that Arabidopsis thaliana Cdc2+/Cdc28 homolog CDKA;1 also phosphorylated phosphorylation at conserved Thr-161 essential its function. A phospho-mimicry T161D substitution restored primary defect cdka;1 mutants, although displayed dramatically reduced kinase...
Abstract Peroxiredoxins (Prx) are thiol-dependent antioxidants containing one (1-cysteine [-Cys]) or two (2-Cys) conserved Cys residues that protect lipids, enzymes, and DNA against reactive oxygen species. In plants, the 1-Cys Prxs highly expressed during late seed development, expression pattern is dormancy related in mature seeds. We have Arabidopsis Prx AtPER1 Escherichia coli show this protein has antioxidant activity vitro protects E. vivo toxic oxidant cumene hydroperoxide. Although...
The Arabidopsis thaliana genome contains more than 30 genes encoding SET-domain proteins that are thought to be epigenetic regulators of gene expression and chromatin structure. can divided into subgroups, members the Polycomb group (PcG) trithorax (trxG) have been shown important development. Both in animals plants some these components multimeric protein complexes. Here, we analyzed trxG ASHR3 which has a SET domain pre- post-SET domains similar Ash1 Drosophila. In addition domain,...
Seed development in angiosperms is dependent on the interplay among different transcriptional programs operating embryo, endosperm, and maternally-derived seed coat. In angiosperms, embryo endosperm are products of double fertilization during which two pollen sperm cells fuse with egg cell central female gametophyte. Arabidopsis, analyses mutants cell-cycle regulator CYCLIN DEPENDENT KINASE A;1 (CKDA;1) have revealed importance a paternal genome for effective ultimately seed. Here we...
The emergence of multicellular animals was associated with an increase in phenotypic complexity and the acquisition spatial cell differentiation embryonic development. Paradoxically, this transition not paralleled by major changes underlying developmental toolkit regulatory networks. In fact, most these systems are ancient, established already unicellular ancestors [1Shalchian-Tabrizi K. Minge M.A. Espelund M. Orr R. Ruden T. Jakobsen K.S. Cavalier-Smith Multigene phylogeny choanozoa origin...
Uridine at the wobble position of tRNA is usually modified, and modification required for accurate efficient protein translation. In eukaryotes, uridines are modified into 5-methoxycarbonylmethyluridine (mcm 5 U), 5-carbamoylmethyluridine (ncm U) or derivatives thereof. Here, we demonstrate, both by in vitro vivo studies, that Arabidopsis thaliana methyltransferase AT1G31600, denoted us AtTRM9, responsible final step mcm U formation, thus representing a functional homologue Saccharomyces...
Abstract The female gametophyte of higher plants gives rise, by double fertilization, to the diploid embryo and triploid endosperm, which develop in concert produce mature seed. What roles gametophytic maternal factors play this process is not clear. female-gametophytic effects on endosperm development Arabidopsis mea, fis, fie mutants appear be due gametic imprinting that can suppressed METHYL TRANSFERASE1 antisense (MET1 a/s) transgene expression or mutation DECREASE IN DNA METHYLATION1...
Abstract The life cycle of plants comprises two alternating generations, the diploid sporophyte (spore-bearing plant) and haploid gametophyte (gamete-bearing plant). In contrast to animals, postmeiotic cells give rise organisms whose function is produce gametes mediate fertilization. Analysis development has been hampered by difficulty identifying haplo-phase-specific mutants in conventional mutagenesis screens. Here we use a genetic strategy that based on segregation distortion nearby...
The control of the stoichiometric balance α- and β-tubulin is important during microtubule biogenesis. This process involves several tubulin-folding cofactors (TFCs), which only TFC A not essential in mammalian vitro systems or vivo yeast. Here, we show that gene plants. Arabidopsis KIESEL (KIS) shows sequence similarity to gene. Expression mouse under 35S promoter rescues kis mutation, indicating KIS ortholog A. plants exhibit a range defects similar phenotypes associated with impaired...
Proteins containing the evolutionarily conserved SET domain are involved in regulation of eukaryotic gene expression and chromatin structure through their histone lysine methyltransferase (HMTase) activity. The Drosophila SU(VAR)3-9 protein related proteins other organisms have been associated with repression heterochromatinization. In Arabidopsis there 10 SUVH 5 SUVR genes encoding similar to SU(VAR)3-9, 4 shown control heterochromatic silencing by its HMTase activity directing DNA...
Genomic imprinting is an epigenetic phenomenon established in the gametes prior to fertilization that causes differential expression of parental alleles, mainly endosperm flowering plants. The overlap between previously identified panels imprinted genes limited. To investigate imprinting, we used high-resolution sequencing data acquired with sequence-capture technology. We present a bioinformatics pipeline assay parent-of-origin allele-specific and report more than 300 loci bias Arabidopsis...
CUL4-RING ubiquitin E3 ligases (CRL4s) were recently shown to exert their specificity through the binding of various substrate receptors, which bind CUL4 interactor DNA DAMAGED BINDING PROTEIN1 (DDB1) a WDxR motif. In segregation-based mutagenesis screen, we identified motif–containing protein (WDR55) required for male and female gametogenesis seed development. We demonstrate that WDR55 physically interacts with Arabidopsis thaliana DDB1A in planta, suggesting may be novel recruiter CRL4...
Abstract Genomic imprinting promotes differential expression of parental alleles in the endosperm flowering plants and is regulated by epigenetic modification such as DNA methylation histone tail modifications chromatin. After fertilization, develops through a syncytial stage before it cellularizes becomes nutrient source for growing embryo. Regional compartmentalization has been shown both early late development, different transcriptional domains suggest divergent spatial temporal regional...