A5039980313- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Wheat and Barley Genetics and Pathology
- Photosynthetic Processes and Mechanisms
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Analysis
- Biochemical and Structural Characterization
- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Enzyme Production and Characterization
- Plant Physiology and Cultivation Studies
- Postharvest Quality and Shelf Life Management
- Antimicrobial Resistance in Staphylococcus
- Phytase and its Applications
- Enzyme Catalysis and Immobilization
- Peptidase Inhibition and Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Reproductive Biology
- Plant Virus Research Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Micronutrient Interactions and Effects
- Plant biochemistry and biosynthesis
- Fermentation and Sensory Analysis
Austrian Centre of Industrial Biotechnology (Austria)
2020-2024
Montana State University
2011-2024
BOKU University
2021-2024
Martin Luther University Halle-Wittenberg
2001-2017
Plant (United States)
2005-2013
Luther University
2013
University of Münster
2002-2012
University Hospital Münster
2007-2012
Heinrich Heine University Düsseldorf
2009-2010
University of Geneva
2008
The fungus Fusarium fujikuroi causes “bakanae” disease of rice due to its ability produce gibberellins (GAs), but it is also known for producing harmful mycotoxins. However, the genetic capacity whole arsenal natural compounds and their role in fungus' interaction with remained unknown. Here, we present a high-quality genome sequence F. that was assembled into 12 scaffolds corresponding chromosomes described fungus. We used along ChIP-seq, transcriptome, proteome, HPLC-FTMS-based metabolome...
Abstract Leaf senescence is a highly organized process and not passive decay. Photosynthesizing mesophyll cells lose their functions in an early phase, while the epidermal layer with stomates phloem remains functional throughout senescence. The subcellular compartmentation maintained allows cooperation of different organelles remobilization constituents. Nitrogen metabolism changes at onset from assimilation to remobilization. Enzymes involved nitrate reduction are lost, some enzymes...
Summary Leaf senescence, the final step of leaf development, involves extensive reprogramming gene expression. Here, we show that these processes include discrete changes epigenetic indexing, as well global alterations in chromatin organization. During interphase nuclei a decondensation chromocenter heterochromatin, and nuclear distribution H3K4me2, H3K4me3, H3K27me2 H3K27me3 histone modification marks index active inactive chromatin, respectively. Locus‐specific indexing was studied at...
Although an involvement of metabolic signals in the regulation plant senescence has been demonstrated a range studies, exact signalling pathways remain largely unresolved. For leaves, evidence supports role sugar accumulation initiation and/or acceleration senescence. However, or ageing may respond to different heterotrophic organs and organisms. In animals yeast, dietary restriction results increased lifespan. this article, leaf is compared with effects restriction. Similarities differences...
ABSTRACT Trimethoprim-sulfamethoxazole (SXT)-resistant Staphylococcus aureus thymidine-dependent small-colony variants (TD-SCVs) are frequently isolated from the airways of cystic fibrosis (CF) patients, often in combination with isogenic normal strains if patients were treated SXT for extended periods. As inhibits synthesis tetrahydrofolic acid, which acts as a cofactor thymidylate synthase ( thyA ), survival TD-SCVs depends exclusively on availability external thymidine. Since underlying...
Leaf senescence can be described as the dismantling of cellular components during a specific time interval before cell death. This has effect remobilizing N in form amino acids that relocalized to developing seeds. High levels carbohydrates have previously been shown promote onset process. Carbohydrate accumulation barley (Hordeum vulgare) plants was induced experimentally by steam-girdling at leaf base, occluding phloem, and gene regulation under these conditions investigated using...
Everybody is familiar with the visual aspects of plant senescence. Color changes occurring in senescing leaves are, however, only one (albeit striking) symptom senescence process. As this process usually leads to cell, tissue, organ or even organism death, its timing critical for fitness and, an agricultural setting, crop performance. It therefore necessary understand mechanisms regulating onset and rate, especially at (for annual species) level. Considering importance fitness, it...
Plant cell vacuoles may have either storage or degradative functions. Vegetative proteins (VSPs) are synthesized in response to wounding and developmental switches that affect carbon nitrogen sinks. Here we show VSPs stored a unique type of vacuole is derived from central coincident with insertion new tonoplast intrinsic protein (TIP), δ-TIP, into their membranes. This finding demonstrates tight coupling between the presence δ-TIP acquisition specialized function indicates TIP isoforms...
• To identify genes involved in the regulation and execution of leaf senescence whole-plant nitrogen reallocation, near-isogenic barley germplasm divergent timing protein concentration mature grains was contrasted. Barley lines differing allelic state at a major locus on chromosome six, controlling grain concentration, were obtained after four generations backcrossing. Based physiological data indicating differences between low- high-grain 14–21 d past anthesis, flag kernel transcriptomes...
• Senescence is the highly regulated last developmental phase of plant organs and tissues, optimized to allow nutrient remobilization surviving parts, such as seeds annual crops. High leaf carbohydrate nitrogen (C : N) ratios have been implicated in induction or acceleration senescence process. A combination phloem interruption mature leaves (by steam-girdling, leading accumulation from photosynthesis) varied nitrate supply was used analyse correlations between metabolite levels, parameters...
Fusion protein technologies to facilitate soluble expression, detection, or subsequent affinity purification in
In this study, full-genome DNA microarrays based on the sequence of Staphylococcus aureus N315 were used to compare transcriptome a clinical S. strain with normal phenotype that its isogenic mutant stable small-colony-variant (SCV) (hemB::ermB). addition standard statistical analyses, systems biology advances applied identify reporter metabolites and achieve more detailed survey genome-wide expression differences between hemB parental strain. Genes enzymes involved in glycolytic fermentative...
Leaf senescence and nitrogen remobilization from senescing tissues are two important factors determining grain protein content (GPC) in cereals. We compared near‐isogenic barley ( Hordeum vulgare L.) germplasm varying the allelic state of a major GPC quantitative trait locus on chromosome 6, delineated by molecular markers HVM74 ABG458 explaining approximately 46% variability this trait. High was consistently associated with earlier whole‐plant senescence. SDS–PAGE immunoblot analysis flag...
Chromatin structure and gene expression are regulated by posttranslational modifications (PTMs) on the N-terminal tails of histones. Mono-, di-, or trimethylation lysine residues histone methyltransferases (HKMTases) can have activating repressive functions depending position context modified lysine. In Arabidopsis, 9 H3 (H3K9me3) is mainly associated with euchromatin transcribed genes, although low levels this mark also detected at transposons repeat sequences. Besides evolutionarily...
ABSTRACT Polyphenol oxidases (PPOs) present in mature wheat ( Triticum aestivum L.) kernels have been implicated the undesirable darkening of cereal products such as Asian noodles. To accelerate functional characterization PPOs and allow identification those PPO genes that are primarily involved food biochemistry, several basic local alignment search tool (BLAST) searches expressed sequence tag (EST) databases were performed using a known argument; identified ESTs resequenced aligned....
Proteins containing the evolutionarily conserved SET domain are involved in regulation of eukaryotic gene expression and chromatin structure through their histone lysine methyltransferase (HMTase) activity. The Drosophila SU(VAR)3-9 protein related proteins other organisms have been associated with repression heterochromatinization. In Arabidopsis there 10 SUVH 5 SUVR genes encoding similar to SU(VAR)3-9, 4 shown control heterochromatic silencing by its HMTase activity directing DNA...
Abstract A gibberellin (GA) biosynthetic pathway was discovered operating in root tips of 7-d-old pumpkin (Cucurbita maxima) seedlings. Stepwise analysis GA metabolism cell-free systems revealed the conversion GA12-aldehyde to bioactive GA4 and inactive GA34. Highest levels endogenous GA34 were found hypocotyls 3-d-old cDNA molecules encoding two oxidases, CmGA20ox3 CmGA3ox3, isolated from LAB150978-treated Recombinant fusion protein converted GA12 GA9, GA24 GA14 GA4, and, less efficiently,...