A5040681676- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
- Genetics and Neurodevelopmental Disorders
- RNA Research and Splicing
- Animal Genetics and Reproduction
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Immune Cell Function and Interaction
- Acute Myeloid Leukemia Research
- Pluripotent Stem Cells Research
- Genetics, Bioinformatics, and Biomedical Research
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Cancer Genomics and Diagnostics
- Genetic Syndromes and Imprinting
- Plant Gene Expression Analysis
- Renal and related cancers
- interferon and immune responses
- RNA and protein synthesis mechanisms
Max Planck Institute of Immunobiology and Epigenetics
2015-2024
Research Institute of Molecular Pathology
2003-2023
Vienna Biocenter
2001-2023
MRC London Institute of Medical Sciences
2022
Hammersmith Hospital
2022
University of Freiburg
2013
Max Planck Society
2008-2013
Dana-Farber Cancer Institute
2005
Harvard University
2005
University of Vienna
2003
Dicer is the enzyme that cleaves double-stranded RNA (dsRNA) into 21–25-nt-long species responsible for sequence-specific RNA-induced gene silencing at transcriptional, post-transcriptional, or translational level. We disrupted dicer-1 ( dcr-1 ) in mouse embryonic stem (ES) cells by conditional targeting and generated Dicer-null ES cells. These were viable, despite being completely defective interference (RNAi) generation of microRNAs (miRNAs). However, mutant displayed severe defects...
Histone lysine methylation is a central modification to mark functionally distinct chromatin regions. In particular, H3-K9 trimethylation has emerged as hallmark of pericentric heterochromatin in mammals. Here we show that H4-K20 also focally enriched at heterochromatin. Intriguingly, by the Suv39h HMTases required for induction trimethylation, although H4 Lys 20 position not an intrinsic substrate these enzymes. By using candidate approach, identified Suv4-20h1 and Suv4-20h2 two novel SET...
Polycomb-group (Pc-G) genes are required for the stable repression of homeotic selector and other developmentally regulated genes, presumably through modulation chromatin domains. Among Drosophila Pc-G Enhancer zeste [E(z)] merits special consideration since it represents one most conserved evolution. In addition, E(Z) protein family contains SET domain, which has recently been linked with histone methyltransferase (HMTase) activity. Although E(Z)-related proteins have not (yet) directly...
The premature aging disease Hutchinson–Gilford Progeria Syndrome (HGPS) is caused by a mutant lamin A (LAΔ50). Nuclei in cells expressing LAΔ50 are abnormally shaped and display loss of heterochromatin. To determine the mechanisms responsible for heterochromatin, epigenetic marks regulating either facultative or constitutive heterochromatin were examined. In from female HGPS patient, histone H3 trimethylated on lysine 27 (H3K27me3), mark lost inactive X chromosome (Xi). methyltransferase...
One function of heterochromatin is the epigenetic silencing by sequestration genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) a major component and thus candidate for establishing maintaining repressive structure. Here we demonstrate that maintenance stable domains in living cells involves transient binding dynamic exchange HP1 from chromatin. kinetics correlate with condensation level chromatin are dependent on histone methyltransferase Suv39h....