A5052252796- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related gene regulation
- Histone Deacetylase Inhibitors Research
- RNA and protein synthesis mechanisms
- Genetics and Neurodevelopmental Disorders
- DNA Repair Mechanisms
- Molecular Biology Techniques and Applications
- Receptor Mechanisms and Signaling
- Catalytic C–H Functionalization Methods
- Sulfur-Based Synthesis Techniques
- Calcium signaling and nucleotide metabolism
- Autophagy in Disease and Therapy
- Protein Degradation and Inhibitors
- Photopolymerization techniques and applications
- Radical Photochemical Reactions
- Cancer-related molecular mechanisms research
- Diabetes Treatment and Management
- Epoxy Resin Curing Processes
- Lignin and Wood Chemistry
- Sirtuins and Resveratrol in Medicine
- Neuropeptides and Animal Physiology
- Mass Spectrometry Techniques and Applications
Sanofi (Germany)
2018-2021
University of Regensburg
2013-2019
Center for Integrated Protein Science Munich
2008-2015
Ludwig-Maximilians-Universität München
2006-2015
University of California, Santa Cruz
2013
Instituto de Salud Carlos III
2007
Spanish National Cancer Research Centre
2003-2005
The present work investigates the occurrence and significance of aberrant DNA methylation patterns during early stages atherosclerosis. To this end, we asked whether genetically atherosclerosis-prone APOE-null mice show any changes in before appearance histologically detectable vascular lesion. We exploited a combination various techniques: fingerprinting, vitro methyl-accepting assay, 5-methylcytosine quantitation, histone post-translational modification analysis, Southern blotting, PCR....
DNA methyltransferase 1 (DNMT1) plays an essential role in murine development and is thought to be the enzyme primarily responsible for maintenance of global methylation status genomic DNA. However, loss DNMT1 human cancer cells affects only a limited number pericentromeric sequences. Here we show that lacking display at least two important differences with respect wild type cells: profound disorganization nuclear architecture, altered pattern histone H3 modification results increase...
The nucleolus is the site of ribosome synthesis in nucleus, whose integrity essential. Epigenetic mechanisms are thought to regulate activity ribosomal RNA (rRNA) gene copies, which part nucleolus. Here we show that human cells lacking DNA methyltransferase 1 (Dnmt1), but not Dnmt33b, have a loss methylation and an increase acetylation level lysine 16 histone H4 at rRNA genes. Interestingly, observed SirT1, NAD+-dependent deacetylase with preference for H4, interacts Dnmt1; SirT1 recruitment...
The AT-hook has been defined as a DNA binding peptide motif that contains glycine-arginine-proline (G-R-P) tripeptide core flanked by basic amino acids. Recent reports documented variations in the sequence of AT-hooks and revealed RNA activity some canonical AT-hooks, suggesting higher structural functional variability this protein domain than previously anticipated. Here we describe discovery characterization extended (eAT-hook), which acids appear symmetrical mainly at distance 12–15 from...
Histone N-termini undergo diverse post-translational modifications that significantly extend the information potential of genetic code. Moreover, they appear to mark specific chromatin regions, modulating epigenetic control, lineage commitment, and overall function chromosomes. It is widely accepted histone affect function, but exact mechanisms how on tails combinations are generated, cross-talk with one another, still enigmatic. Mass spectrometry ideal for analysis becoming gold standard...
Histone post-translational modifications play an important role in regulating chromatin structure and gene expression vivo . Extensive studies investigated the of core histones H3 H4 or linker histone H1. Much less is known on regulation H2A H2B modifications. Here, we show that a major modification Drosophila melanogaster methylation N-terminal proline, which increases during fly development. Experiments performed cultured cells revealed higher levels when are dense, regardless their cell...
Chromatin is the template for replication and transcription in eukaryotic nucleus, which needs to be defined composition structure before these processes can fully understood.We report an isolation protocol targeted purification of specific genomic regions their native chromatin context from Saccharomyces cerevisiae.Subdomains multicopy ribosomal DNA locus containing units RNA polymerases I, II or III autonomous sequence were independently purified sufficient amounts purity analyze protein...
Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, though it known to be essential for embryonic development, its role during adult life still poorly understood. Here we show that this lysine massively hyperacetylated peripheral neutrophils. Genome-wide mapping of H4K16ac terminally differentiated blood cells, along with functional experiments, supported histone post-translational modification the...
Nuclear events such as chromatin condensation, DNA cleavage at internucleosomal sites, and histone release from are recognized hallmarks of apoptosis. However, there is no complete understanding the molecular underlying these changes. It likely that epigenetic changes methylation modifications involved in dynamics structure also nuclear described. In this report we have shown apoptosis associated with global hypomethylation deacetylation leukemia cells. Most importantly, observed a...
The linker histone H1 binds to the DNA in between adjacent nucleosomes and contributes chromatin organization transcriptional control. It is known that carries diverse posttranslational modifications (PTMs), including phosphorylation, lysine methylation ADP-ribosylation. Their biological functions, however, remain largely unclear. This part due fact most of studies have been performed organisms several variants, which complicates analyses. We chosen Drosophila melanogaster, a model organism,...
Abstract Phenol-urea-formaldehyde-tannin (PUFT) adhesives were prepared by co-polymerization of Pinus pinaster bark tannins with phenol-urea-formaldehyde (PUF) prepolymers at room temperature. A detailed analysis 13C-NMR spectroscopy the resins together an evaluation their properties was performed in order to find suitable preparation conditions for prior tannins. allowed identification different linkages formed and quantification main functional groups PUF prepolymers, which greatly...
Abstract Photoredox chemistry has greatly stimulated the application of radical based transformations in medicinal and early drug discovery recent years. Carboxylate groups have been identified as traceless leaving that can be converted into intermediates capable undergoing 1,4‐conjugate addition reactions to Michael acceptors. Herein, we show successful C‐terminal derivatization small peptide substrates by using this methodology a parallel synthesis setting. Finally, outline general...
Abstract Historically, peptide optimization has mainly focused on pharmacological activity. Physicochemical properties have often been evaluated late in discovery, potentially providing molecules with suboptimal stability properties. Often, such issues cannot be overcome by formulation approaches but need additional cycles for a redesign of the peptide. Here, early rational multiparameter exendin‐4 based agonists is described toward i) balanced triple activity profile at glucagon‐like 1,...