A5054993501- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Genomics and Chromatin Dynamics
- Adrenal and Paraganglionic Tumors
- Glioma Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- Molecular Communication and Nanonetworks
- Renal and related cancers
- ATP Synthase and ATPases Research
- Ubiquitin and proteasome pathways
- Lipid Membrane Structure and Behavior
- Wnt/β-catenin signaling in development and cancer
- Nanopore and Nanochannel Transport Studies
- Nanoplatforms for cancer theranostics
- Streptococcal Infections and Treatments
- Cancer Cells and Metastasis
- Lung Cancer Treatments and Mutations
- Fibroblast Growth Factor Research
- Nanoparticle-Based Drug Delivery
- Mass Spectrometry Techniques and Applications
- Cardiac tumors and thrombi
- MicroRNA in disease regulation
- Rabies epidemiology and control
- Luminescence and Fluorescent Materials
- Cancer-related gene regulation
Centre National de la Recherche Scientifique
2020-2024
Institut Pasteur
2020-2024
Université Paris Cité
2021-2024
Instituto de Investigación Sanitaria del Principado de Asturias
2014-2020
Universidad de Oviedo
2013-2019
Central University Hospital of Asturias
2018-2019
Centro de Investigación Biomédica en Red de Cáncer
2019
Glioblastoma (GBM) is the most aggressive brain cancer and its relapse after surgery, chemo radiotherapy appears to be led by GBM stem cells (GSCs). Also, tumor networking intercellular communication play a major role in driving therapy-resistance. Tunneling Nanotubes (TNTs), thin membranous open-ended channels connecting distant cells, have been observed several types of cancer, where they emerge drive more malignant phenotype. Here, we investigated whether are capable intercommunicate...
// Inés Sáenz-de-Santa-María 1 , Cristóbal Bernardo-Castiñeira Eduardo Enciso 2 Inmaculada García-Moreno 3 Jose Luis Chiara 4 Carlos Suarez María-Dolores Servicio de Otorrinolaringología, Hospital Universitario Central Asturias, Instituto Oncología del Principado CIBERONC, Universidad Oviedo, Spain Facultad Ciencias Químicas, Complutense Madrid, Química-Física “Rocasolano”, IQFR-CSIC,...
Loss of 5-hydroxymethylcytosine (5hmC) has been associated with mutations the ten-eleven translocation (TET) enzymes in several types cancer. However, tumors wild-type TET genes can also display low 5hmC levels, suggesting that other mechanisms involved gene regulation might be implicated decline this epigenetic mark. Here we show DNA hypermethylation and loss hydroxymethylation, as well a marked reduction activating histone marks TET3 gene, impair expression lead to genome-wide levels...
// María G. García 1, 2 , Antonella Carella Rocío Urdinguio Gustavo F. Bayón 1 Virginia Lopez Juan Ramón Tejedor 3 Marta I. Sierra Estela García-Toraño Pablo Santamarina Raúl Perez Cristina Mangas Aurora Astudillo 4 M. Daniela Corte-Torres 5 Inés Sáenz-de-Santa-María 6 María-Dolores Chiara Agustín Fernández and Mario Fraga Institute of Oncology Asturias (IUOPA), HUCA, Universidad de Oviedo,...
Tunneling nanotubes (TNTs) are thin membranous channels providing a direct cytoplasmic connection between remote cells. They commonly observed in different cell cultures and increasing evidence supports their role intercellular communication, pathogen amyloid protein transfer. However, the study of TNTs presents several pitfalls (e.g., difficulty preserving such delicate structures, possible confusion with other protrusions, structural functional heterogeneity, etc.) therefore requires...
Metabolic reprogramming is a very heterogeneous phenomenon in cancer. It mostly consists on increased glycolysis, lactic acid formation and extracellular acidification. These events have been associated to activity of the hypoxia inducible factor, HIF-1α. This study aimed at defining metabolic program activated by HIF-1α oropharyngeal squamous cell carcinomas (SCC) assessing its clinical impact. Global gene/miRNA expression was analyzed SCC-derived cells exposed hypoxia. Expression HIF-1α,...
Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, though it known to be essential for embryonic development, its role during adult life still poorly understood. Here we show that this lysine massively hyperacetylated peripheral neutrophils. Genome-wide mapping of H4K16ac terminally differentiated blood cells, along with functional experiments, supported histone post-translational modification the...
Understanding how heterogeneous cancer cell populations migrate collectively is of paramount importance to arrest metastasis. Here, we applied 3D culture-based approaches for in vitro modeling the collective migration squamous carcinoma cells and examine impact epithelial mesenchymal interactions on this type migration. We show that both N-cadherin-expressing cancer-associated fibroblasts cooperate by leading their This was consistent with observed distribution E-cadherin/N-cadherin human...
Abstract Background Cytogenetic and gene expression analyses in head neck squamous cell carcinomas (HNSCC) have allowed identification of genomic aberrations that may contribute to cancer pathophysiology. Nevertheless, the molecular consequences numerous genetic alterations still remain unclear. Methods To identify novel genes implicated HNSCC pathogenesis, we analyzed present five HNSCC-derived lines by array CGH, compared high level focal amplifications with levels whose is directly...
Head and neck paragangliomas (HNPGLs) arise from parasympathetic paraganglias 35% to 45% are hereditary caused by mutations in succinate dehydrogenase (SDH) genes. The connection between SDH tumor development is unclear. most accepted hypothesis proposes a central role for the pseudohypoxic (pHx) pathway activated hypoxia-inducible factor (HIF). Paradoxically, we showed that activation of HIF HNPGLs restricted subset lacking mutations. These tumors overexpress HIF-1α protein target genes...
The hypoxia-inducible factor 1α (HIF-1α) and its microRNA target, miR-210, are candidate tumor-drivers of metabolic reprogramming in cancer. Neuroendocrine neoplasms such as paragangliomas (PGLs) particularly appealing for understanding the cancer adjustments because their associations with deregulations enzymes, succinate dehydrogenase (SDH), von Hippel Lindau (VHL) gene involved HIF-1α stabilization. However, role miR-210 pathogenesis SDH-related tumors remains an unmet challenge. Herein...
Abstract Context Germline mutations in the succinate dehydrogenase A, B, C, and D genes (collectively, SDHx) predispose to development of paragangliomas (PGLs) arising at parasympathetic or sympathetic neuroendocrine systems. SDHx cause absence tumoral immunostaining for SDHB. However, negative SDHB has also been found a subset PGLs that lack mutations. Settings Here, we report comprehensive molecular characterization one such tumor origin compared with healthy paraganglia other without...
We previously showed that activation of epidermal growth factor receptor (EGFR) induces hypoxia inducible factor-1α (HIF-1α) in head and neck squamous cell carcinoma (HNSCC) cells. In this study, we have furthered by investigating the mechanism HIF-1α (EGF) its association with sensitivity to gefitinib.EGFR/HIF-1α signaling was tested immunoblot, polymerase chain reaction (PCR), proliferation, apoptosis assays.HIF-1α accumulated cells overexpressing EGF phosphorylated (pEGFR),...
(–)‐Berkelic acid is an architecturally unique secondary metabolite isolated from extremophilic Penicillium species. In this paper, we describe a very simple protocol to construct the central core of natural product. Using strategy, not only (–)‐berkelic acid, but also small library analogues has been accessed. The key point our remarkable silver‐catalysed cascade reaction that allows assembly product in single step and two starting materials, 4‐pentynol derivative ortho...
Abstract Context SDHB mutations are found in an increasing number of neoplasms, most notably paragangliomas and pheochromocytomas (PPGLs). SDHB-PPGLs slow-growing tumors, but ∼50% them may develop metastasis. The molecular basis metastasis these tumors is a long-standing unresolved problem. Thus, better understanding the biology needed. Objective This study aimed to identify gene methylation changes relevant for metastatic SDHB-PPGLs. Design We performed genome-wide profiling DNA diverse...
A BODIPY derivative of carnitine enters mitochondria regardless their membrane potential and in an enantioselective way through a specific mitochondrial transporter living cells.
Abstract Background Succinate dehydrogenase subunit B (SDHB) immunohistochemistry was considered a valuable tool to identify patients with inherited paraganglioma/pheochromocytoma (PGL/PCC). However, previous studies jointly analyzed 2 related but clinically distinct entities, parasympathetic head and neck paragangliomas (HNPGLs) sympathetic PCCs/PGLs. Additionally, role for hypoxia inducible factor‐1α (HIF‐1α) as biomarker succinate ( SDHx )‐mutated tumors has not been studied. Here, we...
Rabies virus (RABV), the causative agent for rabies disease is still presenting a major public health concern causing approximately 60,000 deaths annually. This neurotropic (genus Lyssavirus , family Rhabdoviridae ) induces an acute and almost always fatal form of encephalomyelitis in humans. Despite lethal consequences associated with clinical symptoms rabies, RABV limits neuro-inflammation without histopathological lesions Nevertheless, information about mechanisms infection cellular...
Metastasis remains a clinically unsolved issue in cancer that is initiated by the acquisition of collective migratory properties cells. Phenotypic and functional heterogeneity arise among cells within same tumor increase cellular plasticity promote metastasis, however, their impact on cell migration incompletely understood. Here, we show vitro depends FAK MMP-2 presence cancer-associated fibroblasts (CAFs). The absence rendered incapable invading surrounding stroma. However, CAFs...
Discoidal nanoparticles showed highest blood-brain barrier permeability and transfer efficiency via tunneling nanotubes in glioblastoma cells, offering a promising strategy for delivery intercellular distribution within tumors.
Abstract Glioblastoma (GBM) is the most aggressive brain cancer and its relapse after surgery, chemo radiotherapy appears to be led by GBM stem cells (GSLCs). Also, tumor networking intercellular communication play a major role in driving therapy-resistance. Tunneling Nanotubes (TNTs), thin membranous open-ended channels connecting distant cells, have been observed several types of cancer, where they emerge drive more malignant phenotype. Here, we investigated whether are capable...