A5088975858- Sarcoma Diagnosis and Treatment
- Genetic and rare skin diseases.
- Psoriasis: Treatment and Pathogenesis
- melanin and skin pigmentation
- Histone Deacetylase Inhibitors Research
- Autoimmune and Inflammatory Disorders
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Autoimmune and Inflammatory Disorders Research
- Genomics and Chromatin Dynamics
- Autoimmune Bullous Skin Diseases
- Cutaneous Melanoma Detection and Management
- Cancer Genomics and Diagnostics
- Reproductive Physiology in Livestock
- Cancer and Skin Lesions
- Asthma and respiratory diseases
- Pneumonia and Respiratory Infections
- Cancer Mechanisms and Therapy
- Multiple Myeloma Research and Treatments
- Nursing care and research
- Neonatal skin health care
- RNA Interference and Gene Delivery
- Genetic and Kidney Cyst Diseases
- Literary and Cultural Studies
- Quinazolinone synthesis and applications
Instituto de Investigación Sanitaria del Principado de Asturias
2014-2023
Universidad de Oviedo
2019-2023
Central University Hospital of Asturias
2019
Universitat Autònoma de Barcelona
2014
Hospital de Manises
2012
Hospital General Universitario De Valencia
2003-2006
The University of Texas at San Antonio
1989
The cellular microenvironment plays a relevant role in cancer development. We have reported that mesenchymal stromal/stem cells (MSCs) deficient for p53 alone or together with RB (p53(-/-)RB(-/-)) originate leiomyosarcoma after subcutaneous (s.c.) inoculation. Here, we show intrabone periosteal inoculation of p53(-/-) p53(-/-)RB(-/-) bone marrow- adipose tissue-derived MSCs originated metastatic osteoblastic osteosarcoma (OS). To assess the contribution environment factors to OS development,...
Abstract Tumors evolve from initial tumorigenic events into increasingly aggressive behaviors in a process usually driven by subpopulations of cancer stem cells (CSCs). Mesenchymal stromal/stem (MSCs) may act as the cell-of-origin for sarcomas and CSCs that present MSC features have been identified due to their ability grow self-renewed floating spheres (tumorspheres). Accordingly, we previously developed sarcoma models using human MSCs transformed with relevant oncogenic events. To study...
// Juan Tornin 1 , Lucia Martinez-Cruzado Laura Santos Aida Rodriguez Luz-Elena Núñez 2 Patricia Oro Maria Ana Hermosilla Eva Allonca Teresa Fernández-García 3 Aurora Astudillo 4 Carlos Suarez Francisco Morís Rene Hospital Universitario Central de Asturias and Instituto Oncología del Principado Asturias, Oviedo, Spain EntreChem SL, Unidad Histopatología Molecular en Modelos Animales Cáncer, IUOPA, Universidad Servicio Anatomía Patológica, Correspondence to: Rodriguez, e-mail: renerg@ficyt.es...
Stemness in sarcomas is coordinated by the expression of pluripotency factors, like SOX2, cancer stem cells (CSC). The role SOX2 tumor initiation and progression has been well characterized osteosarcoma. However, pro-tumorigenic features have scarcely investigated other sarcoma subtypes. Here, we show that depletion dramatically reduced ability undifferentiated pleomorphic (UPS) to form tumorspheres initiate growth. Conversely, overexpression resulted increased vivo tumorigenicity. Moreover,...
Treatment of head and neck squamous cell carcinomas (HNSCC), the sixth most frequent cancer worldwide, remains challenging. miRNA dysregulation is closely linked to tumorigenesis tumor progression, thus emerging as suitable targets for treatment. Transcriptomic analysis TCGA HNSCC dataset revealed that miR-301a expression levels significantly increased in primary tumors, compared patient-matched normal tissue. This prompted us investigate its pathobiological role potential new therapeutic...
Trabectedin has been approved for second-line treatment of soft tissue sarcomas. However, its efficacy to target sarcoma initiating cells not addressed yet. Here, we used pioneer models myxoid/round cell liposarcoma (MRCLS) and undifferentiated pleomorphic (UPS) developed from transformed human mesenchymal stromal/stem (MSCs) evaluate the effect trabectedin in type responsible sarcomagenesis their derived cancer stem (CSC) subpopulations. We found that low nanomolar concentrations...
Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, though it known to be essential for embryonic development, its role during adult life still poorly understood. Here we show that this lysine massively hyperacetylated peripheral neutrophils. Genome-wide mapping of H4K16ac terminally differentiated blood cells, along with functional experiments, supported histone post-translational modification the...
For the cancer genomics era, there is a need for clinically annotated close-to-patient cell lines suitable to investigate altered pathways and serve as high-throughput drug-screening platforms. This particularly important drug-resistant tumors like chondrosarcoma which has few models available. Here we established characterized new derived from two secondary (CDS06 CDS11) one dedifferentiated (CDS-17) chondrosarcomas well another line CDS-17-generated xenograft (T-CDS17). These displayed...
The frequent dysregulation of SRC family kinases (SFK) in multiple cancers prompted various inhibitors to be actively tested preclinical and clinical trials. Disappointingly, dasatinib saracatinib failed demonstrate monotherapeutic efficacy patients with head neck squamous cell carcinomas (HNSCC). Deeper functional mechanistic knowledge the actions these drugs is therefore needed improve outcome develop more efficient combinational strategies. Even though SFK robustly blocked migration...
Cytotoxic drugs like doxorubicin remain as the most utilized agents in sarcoma treatment. However, advanced sarcomas are often resistant, thus stressing need for new therapies aimed to overcome this resistance. Multikinase inhibitors provide an efficient way target several pro‐tumorigenic pathways using a single agent and may constitute valuable strategy treatment of sarcomas, which frequently show aberrant activation pro‐tumoral kinases. Therefore, we studied antitumor activity EC‐70124,...
Background: Stemness in sarcomas is coordinated by the expression of pluripotency factors, like SOX2, cancer stem cell (CSC) subpopulations. The role SOX2 tumor initiation and progression has been well characterized osteosarcoma. However, pro-tumorigenic features have scarcely investigated other sarcoma subtypes.Methods: shRNA cDNA lentiviral constructions were used to deplete overexpress a model undifferentiated pleomorphic (UPS). In addition, we introduced reporter system (SORE6) which...