A5061722080- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Renal and related cancers
- Brucella: diagnosis, epidemiology, treatment
- Circular RNAs in diseases
- Escherichia coli research studies
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Renal cell carcinoma treatment
- Genetic factors in colorectal cancer
- Hepatitis C virus research
- Plant Pathogenic Bacteria Studies
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Legume Nitrogen Fixing Symbiosis
- Molecular Biology Techniques and Applications
- Glioma Diagnosis and Treatment
- Breast Lesions and Carcinomas
- Bacteriophages and microbial interactions
- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- Breast Cancer Treatment Studies
- Burkholderia infections and melioidosis
- Animal Diversity and Health Studies
Biogipuzkoa Health Research Institute
2013-2023
Molecular Oncology (United States)
2017-2018
Polymat
2014
University of the Basque Country
2014
Clinica Universidad de Navarra
2014
Complejo Hospitalario de Navarra
2014
Hospital Universitario Fundación Jiménez Díaz
2014
Pitié-Salpêtrière Hospital
2014
Sorbonne Université
2014
Centro de Investigación Biomédica en Red
2012
Glioblastoma multiforme (GBM) is the most frequent malignant brain tumor in adults, and its prognosis remains dismal despite intensive research therapeutic advances. Diagnostic biomarkers would be clinically meaningful to allow for early detection of those cases which surgery contraindicated or biopsy results are inconclusive. Recent findings show that GBM cells release microvesicles contain a select subset cellular proteins RNA. The aim this hypothesis-generating study was assess diagnostic...
We undertook this study to understand how the transcription factor Sox2 contributes malignant phenotype of glioblastoma multiforme (GBM), most aggressive primary brain tumor. initially looked for unbalanced genomic rearrangements in locus 42 GBM samples and found that was amplified 11.5% overexpressed all samples. These results prompted us further investigate mechanisms involved overexpression GBM. analyzed methylation status promoter because high CpG density promoters are associated with...
Abstract SOX9 [sex-determining region Y (SRY)-box 9 protein], a high mobility group box transcription factor, plays critical roles during embryogenesis and its activity is required for development, differentiation, lineage commitment in various tissues including the intestinal epithelium. Here, we present functional clinical data of broadly important role tumorigenesis. was overexpressed wide range human cancers, where expression correlated with malignant character progression. Gain copy...
Background: SOX2 and SOX9 are commonly overexpressed in glioblastoma, regulate the activity of glioma stem cells (GSCs). Their specific overlapping roles GSCs treatment remain unclear.Methods: levels were examined human biopsies. Gain loss function determined impact altering on cell proliferation, senescence, activity, tumorigenesis chemoresistance.Results: expression correlates positively glioblastoma High bypass cellular senescence promote resistance to temozolomide. Mechanistic...
The Brucella BvrR/BvrS two-component regulatory system is homologous to the ChvI/ChvG systems of Sinorhizobium meliloti and Agrobacterium tumefaciens necessary for endosymbiosis pathogenicity in plants. controls cell invasion intracellular survival. Probing surface bvrR bvrS transposon mutants with monoclonal antibodies showed all described major outer membrane proteins (Omps) but Omp25, a protein known be involved virulence. Absence Omp25 expression was confirmed by two-dimensional...
Abstract Clear cell tubulopapillary renal carcinoma ( CCPRCC ) is a recently described rare malignancy that displays characteristic gross, microscopic and immunohistochemical differences from other tumour types. However, remains very poorly understood entity. We therefore sought to elucidate some of the molecular mechanisms involved in this neoplasm by carrying out targeted next‐generation sequencing NGS identify associated mutations, addition examined expression non‐coding (nc) RNAs ....
The two-component BvrS/BvrR system is essential for Brucella abortus virulence. It was shown previously that its dysfunction abrogates expression of some major outer membrane proteins and increases bactericidal peptide sensitivity. Here, we report mutants have increased surface hydrophobicity susceptibility to killing by nonimmune serum. bvrS bvrR mutant lipopolysaccharides (LPSs) bound more polymyxin B, chimeras constructed with cells parental LPS showed augmented B resistance, and,...
1-42 β-Amyloid (Aβ(1-42)) peptide is a key molecule involved in the development of Alzheimer's disease. Some its effects are manifested at neuronal morphological level. These changes involve loss neurites due to cytoskeleton alterations. However, mechanism Aβ(1-42) activation neurodegenerative program still poorly understood. Here, peptide-induced transduction cellular death signals through phosphatidylinositol 3-kinase (PI3K)/phosphoinositol-dependent kinase (PDK)/novel protein C (nPKC)/Rac...
The circulating transcriptome is a valuable source of cancer biomarkers, which, with the exception microRNAs (miRNAs), remains relatively unexplored. To elucidate which RNAs are present in plasma from melanoma patients and could be used to distinguish healthy individuals, we next generation sequencing (NGS), validation was carried out by qPCR and/or ddPCR. We identified 442 different samples, eleven were differentially expressed (p < 0.05). Levels miR-134-5p miR-320a-3p significantly...
The discovery of the intrarenal renin-angiotensin system (iRAS), which regulates angiogenesis, cell differentiation and proliferation, has opened new perspectives in knowledge kidney carcinogenesis. In this study we analyzed immunohistochemical expression fluorimetric activity four key peptidases iRAS tumor tissue (n = 144) serum samples 128) from patients with renal neoplasms. Neutral endopeptidase (NEP/CD10), Angiotensin-converting enzyme-2 (ACE2), aminopeptidase A (APA) were expressed...
ABSTRACT The Brucella abortus two-component regulatory system BvrR/BvrS controls the expression of outer membrane proteins (Omp) Omp3a (Omp25) and Omp3b (Omp22). Disruption bvrS or bvrR generates avirulent mutants with altered cell permeability, higher sensitivity to microbicidal peptides, complement. Consequently, role in virulence was examined. Similar mutants, omp3a omp3b displayed increased attachment cells, indicating surface alterations. However, they showed unaltered permeability;...
Although it is now evident that microRNAs (miRNAs) play a critical regulatory role in many, if not all, pathological and physiological processes, remarkably they have only formally been recognized for less than fifteen years. These endogenously produced short non-coding RNAs created new paradigm of gene control utility as both novel biomarkers cancer potential therapeutics. In this review we consider the miRNAs lymphoid biology under (i.e. lymphopoiesis) malignant lymphomagenesis)...
The miR-196 family members have been found dysregulated in different cancers. Therefore, they proposed as promising biomarkers and therapeutic targets. This study is the first to investigate role of miR-196b development progression head neck squamous cell carcinomas (HNSCC), also impact on surrounding tumor microenvironment. Increased levels were detected 95% primary tumors precancerous lesions, although no significant differences observed between non-progressing versus progressing...
Treatment of head and neck squamous cell carcinomas (HNSCC), the sixth most frequent cancer worldwide, remains challenging. miRNA dysregulation is closely linked to tumorigenesis tumor progression, thus emerging as suitable targets for treatment. Transcriptomic analysis TCGA HNSCC dataset revealed that miR-301a expression levels significantly increased in primary tumors, compared patient-matched normal tissue. This prompted us investigate its pathobiological role potential new therapeutic...
Bortezomib action in multiple myeloma: microRNA-mediated synergy (and miR-27a /CDK5 driven sensitivity)?
Early diagnosis of laryngeal squamous cell carcinoma (LSCC) at the stage dysplasia could greatly improve outcome affected patients. For first time we compared mutational landscape non-progressing (NPD; n = 42) with progressing (PD; 24), along patient-matched LSCC biopsies; a total 90 samples. Using targeted next-generation sequencing identified non-synonymous mutations in six genes (PIK3CA, FGFR3, TP53, JAK3, MET, FBXW7), and were validated by Sanger and/or qPCR. Analysis was extended silico...
The high levels of interleukin 10 (IL-10) present in chronic hepatitis C virus (HCV) infection have been suggested as responsible for the poor antiviral cellular immune responses found these patients. To overcome immunosuppressive effect IL-10 on antigen-presenting cells such dendritic (DCs), we developed peptide inhibitors to restore DC functions and concomitantly induce efficient responses. Two IL-10-binding peptides (p9 p13) were selected using a phage-displayed library their capacity...