A5043296656- Bioinformatics and Genomic Networks
- Pancreatic and Hepatic Oncology Research
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Advanced Proteomics Techniques and Applications
- Cancer Research and Treatments
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Lung Cancer Treatments and Mutations
- Gene expression and cancer classification
- Phagocytosis and Immune Regulation
- Kruppel-like factors research
- Renal and related cancers
- Melanoma and MAPK Pathways
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Immunotherapy and Biomarkers
- Microtubule and mitosis dynamics
- interferon and immune responses
- Ferroptosis and cancer prognosis
- Ubiquitin and proteasome pathways
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Machine Learning in Bioinformatics
- Cancer Mechanisms and Therapy
Universidad de Navarra
2016-2025
Instituto de Salud Carlos III
2015-2024
Navarre Institute of Health Research
2015-2024
Institute of Bioinformatics
2019
Centro de Investigación Biomédica en Red de Cáncer
2018
Pitié-Salpêtrière Hospital
2014
Polymat
2014
University of the Basque Country
2014
Clinica Universidad de Navarra
2014
Complejo Hospitalario de Navarra
2014
Glioblastoma multiforme (GBM) is the most frequent malignant brain tumor in adults, and its prognosis remains dismal despite intensive research therapeutic advances. Diagnostic biomarkers would be clinically meaningful to allow for early detection of those cases which surgery contraindicated or biopsy results are inconclusive. Recent findings show that GBM cells release microvesicles contain a select subset cellular proteins RNA. The aim this hypothesis-generating study was assess diagnostic...
Diffuse intrinsic pontine glioma (DIPG) is an aggressive brain stem tumor and the leading cause of pediatric cancer-related death. To date, these tumors remain incurable, underscoring need for efficacious therapies. In this study, we demonstrate that immune checkpoint TIM-3 (HAVCR2) highly expressed in both cells microenvironmental cells, mainly microglia macrophages, DIPG. We show inhibition syngeneic models DIPG prolongs survival produces long-term survivors free disease harbor memory....
This research analyzes some aspects of the relationship between gene expression, function, and annotation. Many recent studies are implicitly based on assumption that products biologically functionally related would maintain this similarity both in their expression profiles as well Gene Ontology (GO) We analyze how accurate proves to be using real publicly available data. also aim validate a measure semantic for GO use Pearson correlation coefficient its absolute value products. explore...
Bone metastasis represents one of the most deleterious clinical consequences arising in context many solid tumors. Severe osteolysis results from tumor cell colonization bone compartment, a process which entails reciprocal exchange soluble signals between cells and their osseous microenvironment. Recent evidence indicates that tumor‐intrinsic miRNAs are pleiotropic regulators gene expression. But they also frequently released exosome‐like vesicles (ELV). Yet functional relevance transference...
Abstract KRAS mutated tumours represent a large fraction of human cancers, but the vast majority remains refractory to current clinical therapies. Thus, deeper understanding molecular mechanisms triggered by oncogene may yield alternative therapeutic strategies. Here we report identification common transcriptional signature across mutant cancers distinct tissue origin that includes transcription factor FOSL1. High FOSL1 expression identifies lung and pancreatic cancer patients with worst...
While analyzing the DNA methylome of multiple myeloma (MM), a plasma cell neoplasm, by whole-genome bisulfite sequencing and high-density arrays, we observed highly heterogeneous pattern globally characterized regional hypermethylation embedded in extensive hypomethylation. In contrast to widely reported promoter-associated CpG islands (CGIs) cancer, hypermethylated sites MM, as opposed normal cells, were located outside unexpectedly associated with intronic enhancer regions defined B cells...
With the advent of OMICs technologies, both individual research groups and consortia have spear-headed characterization human samples multiple pathophysiologic origins, resulting in thousands archived genomes transcriptomes. Although a variety web tools are now available to extract information from data, their utility has been limited by capacity nonbioinformatician researchers exploit information. To address this problem, we developed CANCERTOOL, web-based interface that aims overcome major...
Locoregional failure (LRF) in breast cancer patients post-surgery and post-irradiation (IR) is linked to a dismal prognosis. In refined new model, we identified Enpp1 (Ectonucleotide pyrophosphatase /phosphodiesterase 1/CD203a) be closely associated with LRF. Enpp1high circulating tumor cells (CTC) contribute relapse by self-seeding mechanism. This process requires the infiltration of PMN-MDSC neutrophil extracellular traps (NET) formation. Genetic pharmacological inhibition or NET blockade...
To identify tissue microRNAs predictive of sunitinib activity in patients with metastatic renal-cell-carcinoma (MRCC) and to evaluate vitro their mechanism action resistance.We screened 673 using TaqMan Low-density-Arrays (TLDAs) tumors from MRCC extreme phenotypes marked efficacy resistance sunitinib, selected an identification cohort (n = 41). The most relevant differentially expressed were bioinformatics-based target prediction analysis quantified by qRT-PCR presenting similar independent...
Multiple myeloma (MM) is an incurable disease, whose clinical heterogeneity makes its management challenging, highlighting the need for biological features to guide improved therapies. Deregulation of specific long non-coding RNAs (lncRNAs) has been shown in MM, nevertheless, complete lncRNA transcriptome not yet elucidated. In this work, we identified 40,511 novel lncRNAs MM samples. accounted 82% and were more heterogeneously expressed than coding genes. A total 10,351 overexpressed 9,535...
The identification of pancreatic ductal adenocarcinoma (PDAC) dysregulated genes may unveil novel molecular targets entering inhibitory strategies. Laminins are emerging as potential in PDAC given their role diagnostic and prognostic markers. Here, we investigated the cellular, functional, clinical relevance LAMC2 its regulated network, with ultimate goal identifying therapies.LAMC2 expression was analyzed tissues, a panel human mouse cell lines, genetically engineered model. Genetic...
Adenovirus virus-associated (VA) RNAs are processed to functional viral miRNAs or mivaRNAs. mivaRNAs important for virus production, suggesting that they may target cellular genes affect the cell cycle. To look targets of mivaRNAs, we first identified downregulated in presence VA by microarray analysis. These were then screened mivaRNA sites using several bioinformatic tools. The combination analysis and bioinformatics allowed us select splicing translation regulator TIA-1 as a putative...
Glioblastoma multiforme (GBM)-initiating cells (GICs) represent a tumor subpopulation with neural stem cell-like properties that is responsible for the development, progression and therapeutic resistance of human GBM. We have recently shown blockade NFκB pathway promotes terminal differentiation senescence GICs both in vitro vivo, indicating induction may be potential strategy MicroRNAs been implicated pathogenesis GBM, but high-throughput analysis their role GIC has not reported....
Interferons (IFNs) are key players in the antiviral response. IFN sensing by cell activates transcription of IFN-stimulated genes (ISGs) able to induce an state affecting viral replication and release. also induces expression ISGs that function as negative regulators limit strength duration The identified so far belong coding genes. However, only a small proportion transcriptome corresponds transcripts it has been estimated there could be many long non-coding RNAs (lncRNAs). To address...
Type 2 diabetes (T2D) is a complex metabolic disease, which involves maintained hyperglycemia due to the development of an insulin resistance process. Among multiple risk factors, host intestinal microbiota has received increasing attention in T2D etiology and progression. In present study, we have explored effect long-term supplementation with non-dairy fermented food product (FFP) Zucker Diabetic Fatty (ZDF) rats model. The FFP induced improvement glucose homeostasis according results...
Cholangiocarcinoma (CCA) is a neoplasia of the biliary tract driven by genetic, epigenetic and transcriptional mechanisms. Herein, we investigated role transcription factor FOSL1, as well its downstream effectors, in development progression CCA.FOSL1 was human CCA clinical samples. Genetic inhibition FOSL1 mouse cell lines performed vitro vivo models using constitutive inducible short-hairpin RNAs. Conditional ablation done genetically engineered (GEM) model (mutant KRAS Trp53 knockout)....
Abstract Background The discovery of functionally relevant KRAS effectors in lung and pancreatic ductal adenocarcinoma (LUAD PDAC) may yield novel molecular targets or mechanisms amenable to inhibition strategies. Phospholipids availability has been appreciated as a mechanism modulate oncogenic potential. Thus, phospholipid transporters play functional role KRAS-driven oncogenesis. Here, we identified systematically studied the transporter PITPNC1 its controlled network LUAD PDAC. Methods...
Lung cancer is a leading cause of death worldwide. Several alterations in RNA metabolism have been found lung cells; this suggests that metabolism-related molecules are involved the development pathology. In study, we searched for genes exhibit different expression levels between normal and tumor tissues. We identified eight differentially expressed adenocarcinoma microarray datasets. Of these, seven were up-regulated whereas one was down-regulated. Interestingly, most these had not...
Introduction Secreted Protein, Acidic and Rich in Cysteine (SPARC) is a matricellular protein involved many biological processes found over-expressed cirrhotic livers. By mean of genetic approach we herein provide evidence from different vivo liver disease models suggesting profibrogenic role for SPARC. Methods Two fibrosis, based on TAA administration bile duct ligation, were developed SPARC wild-type (SPARC+/+) knock-out (SPARC−/−) mice. Hepatic expression was analyzed by qPCR. Fibrosis...
Vaccination induces immunostimulatory signals that are often accompanied by regulatory mechanisms such as IL-10, which control T-cell activation and inhibit vaccine-dependent antitumor therapeutic effect. Here we characterized IL-10-producing cells in different tumor models treated with vaccines. Although several cell subsets produced IL-10 irrespective of treatment, an early induction was detected dendritic (DC). production defined a DC population poorly mature phenotype, lower expression...
Sunitinib is one of the most widely used targeted therapeutics for renal cell carcinoma (RCC), but acquired resistance against therapies remains a major clinical challenge. To dissect mechanisms and unravel reliable predictive biomarkers sunitinib in RCC, we sequenced exons 409 tumor-suppressor genes oncogenes paired tumor samples from an RCC patient, obtained at baseline after development to sunitinib. From newly arising mutations, selected, using silico prediction models, six predicted be...
Few therapies are currently available for patients with KRAS-driven cancers, highlighting the need to identify new molecular targets that modulate central downstream effector pathways. Here we found microRNA (miRNA) cluster including miR181ab1 is a key modulator of oncogenesis. Ablation Mir181ab1 in genetically engineered mouse models Kras-driven lung and pancreatic cancer was deleterious tumor initiation progression. Expression both resident miRNAs cluster, miR181a1 miR181b1, necessary...