Vincenzo Corbo
A5024767391- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Neuroendocrine Tumor Research Advances
- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- Lung Cancer Research Studies
- Lung Cancer Treatments and Mutations
- Phagocytosis and Immune Regulation
- Renal and related cancers
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Immune cells in cancer
- Genetic factors in colorectal cancer
- PI3K/AKT/mTOR signaling in cancer
- RNA modifications and cancer
- Neuroblastoma Research and Treatments
- Pancreatic function and diabetes
- Genomics and Phylogenetic Studies
- Immunotherapy and Immune Responses
- Genomics and Chromatin Dynamics
- Extracellular vesicles in disease
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- Genomics and Rare Diseases
- Protein Degradation and Inhibitors
University of Verona
2016-2025
Agostino Gemelli University Polyclinic
2021
University of L'Aquila
2021
Istituto Dermopatico dell'Immacolata
2021
Catholic University of America
2021
Istituti di Ricovero e Cura a Carattere Scientifico
2021
Istituto Tumori Bari
2021
University Hospital Foundation
2019-2021
CS Diagnostics
2021
Lustgarten Foundation
2014-2019
Pancreatic stellate cells (PSCs) differentiate into cancer-associated fibroblasts (CAFs) that produce desmoplastic stroma, thereby modulating disease progression and therapeutic response in pancreatic ductal adenocarcinoma (PDA). However, it is unknown whether CAFs uniformly carry out these tasks or if subtypes of with distinct phenotypes PDA exist. We identified a CAF subpopulation elevated expression α-smooth muscle actin (αSMA) located immediately adjacent to neoplastic mouse human...
Histopathological samples are a treasure-trove of DNA for clinical research. However, the quality can vary depending on source or extraction method applied. Thus standardized and cost-effective workflow qualification preparations is essential to guarantee interlaboratory reproducible results. The process consists quantification double strand (dsDNA) assessment its suitability downstream applications, such as high-throughput next-generation sequencing. We tested two most frequently used...
Abstract Heterogeneous subtypes of cancer-associated fibroblasts (CAFs) coexist within pancreatic cancer tissues and can both promote restrain disease progression. Here, we interrogate how cells harboring distinct alterations in p53 manipulate CAFs. We reveal the existence a p53-driven hierarchy, where with gain-of-function (GOF) mutant educate dominant population CAFs that establish pro-metastatic environment for GOF null alike. also demonstrate educated by may be reprogrammed either or...
The importance of epigenetic modifications such as DNA methylation in tumorigenesis is increasingly being appreciated. To define the genome-wide pattern pancreatic ductal adenocarcinomas (PDAC), we captured profiles 167 untreated resected PDACs and compared them to a panel 29 adjacent nontransformed pancreata using high-density arrays. A total 11,634 CpG sites associated with 3,522 genes were significantly differentially methylated (DM) PDAC capable segregating from non-malignant pancreas,...
Next-generation sequencing (NGS) was applied to 148 lung neuroendocrine tumours (LNETs) comprising the four World Health Organization classification categories: 53 typical carcinoid (TCs), 35 atypical (ACs), 27 large-cell carcinomas, and 33 small-cell carcinomas. A discovery screen conducted on 46 samples by use of whole-exome high-coverage targeted 418 genes. Eighty-eight recurrently mutated genes from both current literature were verified in cases screen, validated additional 102 LNETs...
// Michele Simbolo 1,2,* , Matteo Fassan Andrea Ruzzenente 3,* Mafficini 1 Laura D. Wood 4 Vincenzo Corbo Davide Melisi 5 Giuseppe Malleo 6 Caterina Vicentini Giorgio Malpeli 1,3,6 Antonello Nicola Sperandio Paola Capelli 2 Anna Tomezzoli Calogero Iacono 3 Rita T. Lawlor 1,2 Claudio Bassi Ralph H. Hruban Alfredo Guglielmi Giampaolo Tortora Filippo de Braud 7 Aldo Scarpa ARC-Net Research Centre, University and Hospital Trust of Verona, Italy Department Pathology Diagnostics, Surgery, General...
<h3>Background</h3> Pancreatic ductal adenocarcinoma (PDAC) is a highly devastating disease with an overall 5-year survival rate of less than 8%. New evidence indicates that PDAC cells release pro-inflammatory metabolites induce marked alteration normal hematopoiesis, favoring the expansion and accumulation myeloid-derived suppressor (MDSCs). We report here patients show increased levels both circulating tumor-infiltrating MDSC-like cells. <h3>Methods</h3> The frequency MDSC subsets in...
Objective Recent studies have found aristaless-related homeobox gene (ARX)/pancreatic and duodenal 1 (PDX1), alpha-thalassemia/mental retardation X-linked (ATRX)/death domain-associated protein (DAXX) alternative lengthening of telomeres (ALT) to be promising prognostic biomarkers for non-functional pancreatic neuroendocrine tumours (NF-PanNETs). However, they not been comprehensively evaluated, especially among small NF-PanNETs (≤2.0 cm). Moreover, their status in (NETs) from other sites...
Purpose Individuals with adenocarcinoma of the ampulla Vater demonstrate a broad range outcomes, presumably because these cancers may arise from any one three epithelia that converge at location. This variability poses challenges for clinical decision making and development novel therapeutic strategies. Patients Methods We assessed potential utility histomolecular phenotypes defined using combination histopathology protein expression (CDX2 MUC1) in 208 patients independent cohorts who...
Pancreatic endocrine tumors (PETs) may be part of hereditary multiple neoplasia type 1 (MEN1) syndrome. While MEN1 gene mutation is the only ascertained genetic anomaly described in PETs, no data exist on cellular localization MEN1-encoded protein, menin, normal pancreas and PETs. A total 169 PETs were used to assess i) mutational status 100 clinically sporadic by direct DNA sequencing, ii) immunohistochemical expression menin 140 including 71 cases screened for mutations, iii) correlation...
Abstract The CRISPR/Cas9 system is a powerful tool for studying gene function. Here, we describe method that allows temporal control of activity based on conditional Cas9 destabilization. We demonstrate fusing an FKBP12-derived destabilizing domain to (DD-Cas9) enables expression and editing in the presence FKBP12 synthetic ligand. This can be easily adapted co-express, from same promoter, DD-Cas9 with any other interest without co-modulation latter. In particular, when co-expressed...
DNA mutational profiling showed that atypical carcinoids (ACs) share alterations with large cell neuroendocrine carcinomas (LCNECs). Transcriptomic studies suggested LCNECs are composed of two subtypes, one which shares molecular anomalies SCLC. The missing piece information is the transcriptomic relationship between ACs and LCNECs, as a direct comparison lacking in literature.Transcriptomic genomic were investigated by next-generation sequencing discovery set 14 validated on 21 18 using...
Abstract The existing Intraductal Papillary Mucinous Neoplasm (IPMN) risk stratification relies on clinical and histological factors, resulting in inaccuracies leading to suboptimal treatment. This is due the lack of appropriate molecular markers that can guide patients toward best therapeutic options. Here, we assess confirm subtype-specific for IPMN across two independent cohorts using Spatial Transcriptomics (ST) technologies. Specifically, identify HOXB3 ZNF117 as Low-Grade Dysplasia,...
Abstract Purpose: KRAS is mutated in the majority of pancreatic ductal adenocarcinoma. MAPK and PI3K-AKT are primary effector pathways, but combined PI3K inhibition has not been demonstrated to be clinically effective date. We explore resistance mechanisms uniquely employed by malignant cells. Experimental Design: evaluated expression activation receptor tyrosine kinases response MEK AKT KPC mice organoids. In addition, we sought determine therapeutic efficacy targeting pathways induced...
Background Complex tumor and immune microenvironment render pancreatic ductal adenocarcinoma (PDAC) resistant to checkpoint inhibitors (ICIs). Therefore, a strategy convert the hostile into an immunopermissive is required. Recent studies showed that intratumoral injection of Toll-like receptor 9 agonist IMO-2125 primes adaptive response. Phase I II trials with demonstrated its safety antitumoral activity. Methods We generated array preclinical models by orthotopically engrafting PDAC-derived...