Andrew Ruszkiewicz
A5060655524- Gastric Cancer Management and Outcomes
- Colorectal Cancer Screening and Detection
- Genetic factors in colorectal cancer
- Esophageal Cancer Research and Treatment
- Pancreatic and Hepatic Oncology Research
- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Colorectal Cancer Surgical Treatments
- Epigenetics and DNA Methylation
- Eosinophilic Esophagitis
- Gastrointestinal disorders and treatments
- Esophageal and GI Pathology
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Gastrointestinal Tumor Research and Treatment
- Neuroendocrine Tumor Research Advances
- Radiomics and Machine Learning in Medical Imaging
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Eosinophilic Disorders and Syndromes
- RNA modifications and cancer
- Lung Cancer Diagnosis and Treatment
- Gastroesophageal reflux and treatments
- Liver Disease Diagnosis and Treatment
- Restraint-Related Deaths
- Helicobacter pylori-related gastroenterology studies
South Australia Pathology
2015-2024
The University of Adelaide
2013-2024
University of South Australia
2014-2024
Centre for Cancer Biology
2014-2024
Royal Adelaide Hospital
2012-2023
Liechtenstein Institute
2023
Lyell McEwin Hospital
2013-2022
RELX Group (Netherlands)
2019
Chinese University of Hong Kong
2018
Creative Research Enterprises (United States)
2018
Activation of the canonical TGF-β signaling pathway provides growth inhibitory signals in normal intestinal epithelium. Colorectal cancers (CRCs) frequently harbor somatic mutations members TGFBR2 and SMAD4, but to what extent SMAD2 or SMAD3 contribute tumorigenesis is unclear. A cohort 744 primary CRCs 36 CRC cell lines were sequenced for SMAD2, analyzed allelic loss by single-nucleotide polymorphism (SNP) microarray analysis. Mutation spectra compared between genes, pathogenicity was...
Abstract Heterogeneous subtypes of cancer-associated fibroblasts (CAFs) coexist within pancreatic cancer tissues and can both promote restrain disease progression. Here, we interrogate how cells harboring distinct alterations in p53 manipulate CAFs. We reveal the existence a p53-driven hierarchy, where with gain-of-function (GOF) mutant educate dominant population CAFs that establish pro-metastatic environment for GOF null alike. also demonstrate educated by may be reprogrammed either or...
Abstract The lysyl oxidase family represents a promising target in stromal targeting of solid tumors due to the importance this crosslinking and stabilizing fibrillar collagens its known role tumor desmoplasia. Using small-molecule drug-design approaches, we generated validated PXS-5505, first-in-class highly selective potent pan-lysyl inhibitor. We demonstrate vitro vivo that inhibition decreases chemotherapy-induced pancreatic desmoplasia stiffness, reduces cancer cell invasion metastasis,...
<h3>AIM</h3> Colorectal cancer has been described in association with hyperplastic polyposis but the mechanism underlying this observation is unknown. The aim of study was to characterise foci dysplasia developing polyps subjects on basis DNA microsatellite status and expression mismatch repair proteins hMLH1, hMSH2, hMSH6. <h3>MATERIALS AND METHODS</h3> material derived from four patients between one six synchronous colorectal cancers. Normal (four), (13), dysplastic malignant (11) samples...
Abstract Background BRAF is a member of RAF family serine/threonine kinases and mediates cellular responses to growth signals through the RAS-RAF-MAP kinase pathway. Activating mutations in have recently been found about 10% colorectal cancers, with vast majority being V600E hotspot mutation. The aim present study was evaluate clinical, pathological molecular phenotype tumors mutations. Results Mutations were identified 8% (23/275) cancers. They 5–10-fold more frequent infiltrating...
Cancer progression is a complex series of events thought to incorporate the reversible developmental process epithelial-to-mesenchymal transition (EMT). In vitro, microRNA-200 family maintains epithelial phenotype by posttranscriptionally inhibiting E-cadherin repressors, ZEB1 and ZEB2. Here, we used in situ hybridization immunohistochemistry assess expression miR-200 EMT biomarkers formalin-fixed paraffin-embedded human colorectal adenocarcinomas. addition, laser capture microdissection...
PIK3CA and PTEN mutations are prevalent in colorectal cancer potential markers of response to mitogen-activated protein/extracellular signal-regulated kinase inhibitors anti-EGF receptor antibody therapy. Relationships between phosphoinositide 3-kinase (PI3K) pathway mutation, clinicopathologic characteristics, molecular features, prognosis remain controversial.A total 1,093 stage I-IV cancers were screened for (exons 9 20), KRAS (codons 12-13), BRAF (codon 600) mutations, microsatellite...
Background Chimeric antigen receptor (CAR) T cell therapies specific for the CD19 and B-cell maturation have become an approved standard of care worldwide relapsed refractory malignancies. If CAR-T therapy non-hematological malignancies is to achieve same stage clinical development, then iterative early-phase testing can add value development process evaluating products containing different CAR designs manufactured under differing conditions. Methods We conducted a phase 1 trial...
The CpG-island methylator phenotype (CIMP+) in colorectal cancer (CRC) is characterised by frequent hypermethylation of promoter regions tumour suppressor genes. Low level methylation some CpG islands also seen the normal colonic mucosa and increases with age; however, it still unclear what other factors regulate this phenomenon. first aim our study was to determine whether elevated patients CIMP+ tumours. second investigate common, functional polymorphisms genes involved methyl group...
Abstract This study characterized the contribution of bone marrow-derived cells to human neoplasia and perineoplastic stroma. The Australasian Bone Marrow Transplant Recipient Registry was used identify solid organ that developed in female recipients male allogeneic stem cell transplants. Eighteen suitable cases were identified including several skin cancers, two gastric one rectal adenoma. Light microscopy, fluorescence chromogenic situ hybridization, immunohistochemistry performed...
Summary Hepatocyte clone size was measured in liver samples of 21 patients various stages chronic hepatitis B virus ( HBV ) infection and from to 76 years age. clones containing unique virus–cell DNA junctions formed by the integration were detected using inverse nested PCR . The maximum hepatocyte tended increase with age, although there considerable patient‐to‐patient variation each age group. There an upward trend increasing fibrosis, inflammatory activity seroconversion e‐antigen HB e A...
The most common of all activating BRAF mutations (T1799A) leads to a substitution valine (V) glutamic acid (E) at the position 600 amino sequence. major goal this study was compare detection V600E mutation by DNA sequencing with immunohistochemistry (IHC) using anti-BRAF (VE1) antibody. Archival formalin fixed, paraffin embedded tissues from 352 patients colon adenocarcinoma (n = 279) and papillary thyroid carcinoma 73) were evaluated for IHC. discordant cases re-evaluated repeat IHC,...
The majority of colorectal cancer (CRC) cases are preventable by early detection and removal precancerous polyps. Even though CRC is the second most common internal in Australia, only 30 per cent population considered to have risk factors participate stool-based test screening programs. Evidence indicates a robust, blood-based, diagnostic assay would increase compliance. A number potential blood-based protein biomarkers for been reported, but all lack sensitivity or specificity use as...
Here we report the DNA methylation profile of 84 sporadic pancreatic neuroendocrine tumors (PanNETs) with associated clinical and genomic information. We identified three subgroups PanNETs, termed T1, T2 T3, distinct patterns methylation. The T1 subgroup was enriched for functional ATRX, DAXX MEN1 wild-type genotypes. contained mutations in recurrent chromosomal losses half genome no association between regions loss levels. were larger had lower MGMT gene body, which showed positive...
The factors that regulate lymphocyte traffic in chronic hepatitis C (CHC) are not completely defined. Interferon (IFN)-inducible T cell α chemoattractant (I-TAC) is a relatively new member of the CXCR3 chemokine ligand family selectively recruits activated cells to sites inflammation. To determine if I-TAC plays role CHC, we investigated expression virus (HCV)-infected liver biopsy material. messenger RNA (mRNA) levels were significantly increased HCV-infected compared with normal liver,...
Hypermethylation of CpG island loci within gene promoter regions is a frequent event in colorectal cancer that often associated with transcriptional silencing and has been referred to as CIMP+. DNA hypomethylation can occur concert CIMP+, although these two phenomena appear not be related cancer. The authors investigated here whether the methylation level LINE‐1 repeats, surrogate marker for genomic methylation, was cancers matching normal colonic mucosa from 178 patients. MethyLight assay...