A5061700388- TGF-β signaling in diseases
- Extracellular vesicles in disease
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- Cytokine Signaling Pathways and Interactions
- Ubiquitin and proteasome pathways
- Cell Adhesion Molecules Research
- MicroRNA in disease regulation
- Plant and Fungal Interactions Research
- Plant Virus Research Studies
- HER2/EGFR in Cancer Research
- Protein Kinase Regulation and GTPase Signaling
- Lung Cancer Treatments and Mutations
- Renal and related cancers
- Glioma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Cancer Mechanisms and Therapy
- Signaling Pathways in Disease
- Ferroptosis and cancer prognosis
- Chronic Kidney Disease and Diabetes
- Pancreatitis Pathology and Treatment
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
The Royal Melbourne Hospital
2016-2025
The University of Melbourne
2016-2025
University of Electronic Science and Technology of China
2013-2024
Xuzhou Central Hospital
2024
Taiyuan Maternity and Child Care Hospital
2024
Second Affiliated Hospital of Nanchang University
2024
Nanchang University
2024
Harbin Medical University
2009-2023
Fujian Medical University
2023
First Affiliated Hospital of Harbin Medical University
2009-2023
Exosomes are small extracellular 40-100 nm diameter membrane vesicles of late endosomal origin that can mediate intercellular transfer RNAs and proteins to assist premetastatic niche formation. Using primary (SW480) metastatic (SW620) human isogenic colorectal cancer cell lines we compared exosome protein profiles yield valuable insights into factors signaling molecules fundamental tumor progression. purified using OptiPrep™ density gradient fractionation were in diameter, a buoyant ~1.09...
Activation of the canonical TGF-β signaling pathway provides growth inhibitory signals in normal intestinal epithelium. Colorectal cancers (CRCs) frequently harbor somatic mutations members TGFBR2 and SMAD4, but to what extent SMAD2 or SMAD3 contribute tumorigenesis is unclear. A cohort 744 primary CRCs 36 CRC cell lines were sequenced for SMAD2, analyzed allelic loss by single-nucleotide polymorphism (SNP) microarray analysis. Mutation spectra compared between genes, pathogenicity was...
Tumor cells in ascites are a major source of disease recurrence ovarian cancer patients. In an attempt to identify and profile the population obtained from patients, novel method was developed separate adherent (AD) non-adherent (NAD) culture. Twenty-five patients were recruited this study; 11 chemonaive (CN) 14 chemoresistant (CR). AD both CN CR exhibited mesenchymal morphology with antigen stem fibroblasts. Conversely, NAD had epithelial enhanced expression 125 (CA125), cell adhesion...
ABSTRACT. TGF-β is a key mediator in renal fibrosis. Kidney-targeted gene therapy with anti–TGF-β strategies expected to have therapeutic potential, but this has been hampered by concerns over the safety and practicability of viral vectors inefficiency nonviral transfection techniques. The present study explored potential role TGF-β/Smad signaling fibrosis vivo developed safe effective specifically block rat unilateral ureteral obstruction (UUO) model transferring doxycycline-regulated Smad7...
While it is thought that advanced glycation end products (AGEs) act by stimulating transforming growth factor (TGF)-beta to mediate diabetic injury, we report AGEs can activate TGF-beta signaling, Smads, and scarring directly independently of TGF-beta. Smad2/3 in renal vascular cells at 5 min, peaking over 15-30 min before synthesis 24 h occurs receptor I II mutant cells. This mediated RAGE ERK/p38 mitogen-activated protein kinases (MAPKs). In addition, also Smads via the classic...
It has been shown that transforming growth factor-beta (TGF-beta) is a potent mediator in renal fibrosis and Smad proteins are critical intracellular mediators TGF-beta signaling. here reported mediates fibrogenesis tubular epithelial cells (TEC) association with the activation of Smad2 overexpression Smad7 blocks this fibrotic process. Using normal rat kidney cell line (NRK52E), it was determined TGF-beta1 induces phosphorylation nuclear localization both dose- time-dependent manner. The...
The type III transforming growth factor β (TGFβ) receptor (TβRIII) binds both TGFβ and inhibin with high affinity modulates the association of these ligands their signaling receptors. However, significance TβRIII in vivo is not known. In this study, we have sought to determine role during development. We identified predominant expression sites ΤβRIII mRNA as liver heart midgestation disrupted murine gene by homologous recombination. Beginning at embryonic day 13.5, mice mutations developed...
Abstract Over 80% of women diagnosed with advanced-stage ovarian cancer die as a result disease recurrence due to failure chemotherapy treatment. In this study, using two distinct cell lines (epithelial OVCA 433 and mesenchymal HEY) we demonstrate enrichment in population cells high expression CSC markers at the protein mRNA levels response cisplatin, paclitaxel combination both. We also significant enhancement sphere forming abilities drugs. The results these vitro findings are supported by...
Epithelial-mesenchymal transition (EMT) is a highly conserved morphogenic process defined by the loss of epithelial characteristics and acquisition mesenchymal phenotype. EMT associated with increased aggressiveness, invasiveness, metastatic potential in carcinoma cells. To assess contribution extracellular vesicles following EMT, we conducted proteomic analysis exosomes released from Madin-Darby canine kidney (MDCK) cells, MDCK cells transformed oncogenic H-Ras (21D1 cells). Exosomes are...
Abstract Idiopathic pulmonary fibrosis (IPF) is a fatal disease that unresponsive to current therapies and characterized by excessive collagen deposition subsequent fibrosis. While inflammatory cytokines, including interleukin (IL)‐6, are elevated in IPF, the molecular mechanisms underlie this incompletely understood, although development of believed depend on canonical transforming growth factor (TGF)‐β signalling. We examined bleomycin‐induced inflammation mice carrying mutation shared...
1Department of Biochemistry &Molecular Biology, Bio21Molecular Sciences and Biotechnology Institute, The University Melbourne, Parkville, VIC 3010, Australia 2Department Biochemistry, Hong Kong Science Technology, Clear Water Bay, Kowloon, Kong, China 3Bloomfield Center for Research in Aging, Lady Davis Institute Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, Montreal, QC, Canada H3T 1E2 4The Department Neurology Neurosurgery, McGill University, 5Department Surgery, Royal...
Abstract Background Bone morphogenetic proteins (BMPs) have been reported to maintain epithelial integrity and antagonize the transforming growth factor β (TGFβ)-induced mesenchymal transition. The expression of soluble BMP antagonists is dysregulated in cancers interrupts proper signaling breast cancer. Methods In this study, we mined prognostic role GREMLIN 1 ( GREM1 ) primary cancer tissues using in-house publicly available datasets. We determined which cells express RNA situ...
Current treatment of ovarian cancer patients with chemotherapy leaves behind a residual tumor which results in recurrent within short time frame. We have previously demonstrated that single short-term cells vitro resulted stem cell (CSC)-like enriched population generated significantly greater burden compared to the by control untreated cells. In this report we looked at mechanisms enrichment CSC-like response paclitaxel treatment. The mechanism survival paclitaxel-treated growth inhibitory...
Abstract Purpose: Therapies directed to specific molecular targets are still unmet for patients with triple-negative breast cancer (TNBC). Deubiquitinases (DUB) emerging drug targets. The identification of highly active DUBs in TNBC may lead novel therapies. Experimental Design: Using DUB activity probes, we profiled global activities 52 cell lines and patients' tumor tissues. To validate our findings vivo, employed both zebrafish murine xenograft models. Cellular mechanisms were elucidated...
// Jayanthi Lea 1 , Raghava Sharma 2 Fan Yang Hong Zhu E. Sally Ward 3,4 and Alan J. Schroit 1,3 Harold Simmons Comprehensive Cancer Center, UT Southwestern Medical Dallas, TX, USA Hamon Center for Therapeutic Oncology Research, 3 Department of Immunology, 4 Molecular Cellular Medicine, Texas A&M University Health Science College Station, Correspondence to: Schroit, email: Keywords : phosphatidylserine, extracellular vesicles, ovarian malignancies, exosomes Received January 04, 2017 Accepted...