Epidermal growth factor receptor (EGFR) is overexpressed in ovarian carcinomas, with direct or indirect activation of EGFR able to trigger tumour growth. We demonstrate significant both signal transducer and activator transcription (STAT)3 its upstream Janus kinase (JAK)2, high-grade carcinomas compared normal ovaries benign tumours. The association between STAT3 migratory phenotype cancer cells was investigated by EGF-induced epithelial–mesenchymal transition (EMT) OVCA 433 SKOV3 cell...

Epithelial mesenchymal transition (EMT) and cancer stem cells (CSC) have been associated with resistance to chemotherapy. Eighty percent of ovarian patients initially respond platinum-based combination therapy but most return recurrence ultimate demise. To better understand such chemoresistance we assessed the potential role EMT in tumor collected from advanced-stage cell line OVCA 433 response cisplatin vitro. We demonstrate that cisplatin-induced epithelial morphology residual correlated...

Tumor cells in ascites are a major source of disease recurrence ovarian cancer patients. In an attempt to identify and profile the population obtained from patients, novel method was developed separate adherent (AD) non-adherent (NAD) culture. Twenty-five patients were recruited this study; 11 chemonaive (CN) 14 chemoresistant (CR). AD both CN CR exhibited mesenchymal morphology with antigen stem fibroblasts. Conversely, NAD had epithelial enhanced expression 125 (CA125), cell adhesion...

Airborne bacteria isolated from a tannery air environment were screened for the property of solubilization insoluble zinc oxide and phosphate. Out 10 strains tested, strain Pseudomonas aeruginosa (CMG 823) showed best solubilized both Colonies bacterium produced clear haloes on solid medium which contained these metal compounds, but only when glucose was provided as carbon source. Solubilization phosphate accompanied by an increase in H+ concentration medium, probably consequence production...

The ovarian surface epithelium (OSE) is the precursor of common epithelial carcinomas. In present study, we examined molecular mechanisms and possible physiological basis for propensity OSE cells to undergo epithelio-mesenchymal transition (EMT) in response environmental influences. We hypothesized that EMT may be a homeostatic mechanism permits displaced assume stromal phenotype within cortex. report EGF conjunction with hydrocortisone EMT-inducing factor as shown by changes fibroblast-like...

Abstract Over 80% of women diagnosed with advanced-stage ovarian cancer die as a result disease recurrence due to failure chemotherapy treatment. In this study, using two distinct cell lines (epithelial OVCA 433 and mesenchymal HEY) we demonstrate enrichment in population cells high expression CSC markers at the protein mRNA levels response cisplatin, paclitaxel combination both. We also significant enhancement sphere forming abilities drugs. The results these vitro findings are supported by...

Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these in the search for potential diagnostic/prognostic biomarkers serum patients has been limited by presence highly abundant albumin immunoglobulins that constitute approximately 60-97% total proteins. purpose study was evaluate whether treatment human with Affi-Gel Blue alone or combination Protein A (Aurum protein mini kit, Bio-Rad) before two-dimensional gel electrophoresis...

The expression of neutrophil gelatinase-associated lipocalin (NGAL) has been shown to be upregulated in ovarian cancer cells. In this study, we report that the immunoreactive NGAL (irNGAL) tumors changes with disease grade and change is reflected concentration peripheral blood. A total 59 tissues including normal, benign, borderline malignant grades 1, 2 3 were analyzed using immunohistochemistry. irNGAL was not present normal ovaries weak moderate benign tissues. Both 1 displayed highest...

On the basis of high enzyme activity a newly isolated strain L. mesenteroides CMG713 was selected for dextran production. For maximum yield dextran, effects various parameters such as pH, temperature, sucrose concentration and incubation period were studied. produced after 20 hours at 30 masculineC with 15% pH 7.0. The molecular mass distribution by this showed that its about 2.0 million Da. Dextran analysis (13)C-NMR spectrometry no signals corresponding to any other linkages except...

Indigenous soil samples were tested for Phosphate solubilization. Efficient phosphate solubilizng bacteria isolated. Effect of four different media on solubilization determined. Auxin production by these determined via bioassay and high performance liquid chromatography the in culture. Indole acetic acid indole butyric produced varying concentration with out addition tryptophan. These showed stimulatory effects growth root shoot elongation mung beans (Vigna radiata). Three promising isolates...

Chemotherapy using cytotoxic agents, such as paclitaxel (PTX), is one of the most effective treatments for advanced ovarian cancer. However, due to nonspecific targeting drug and presence toxic solvents required dissolving PTX prior injection, there are several serious side effects associated with this treatment. In study, we explored self-assembled lipid-based nanoparticles carriers, which were able improve its antitumour efficacy against The also functionalized epidermal growth factor...

Current treatment of ovarian cancer patients with chemotherapy leaves behind a residual tumor which results in recurrent within short time frame. We have previously demonstrated that single short-term cells vitro resulted stem cell (CSC)-like enriched population generated significantly greater burden compared to the by control untreated cells. In this report we looked at mechanisms enrichment CSC-like response paclitaxel treatment. The mechanism survival paclitaxel-treated growth inhibitory...

Epithelial ovarian cancer is the fourth leading cause of death among women. Due to asymptomatic nature and poor survival characteristic disease, screening for specific biomarkers a major health priority. Differentially expressed proteins in serum patients have potential be used as cancer-specific biomarkers. In this study, proteomic methods were screen 24 samples from women with high-grade compared control group 11 healthy Affigel-Blue treated processed either by linear (pH 4–7) or narrow...

Background: Ovarian cancer is characterized by a wide-spread intra-abdominal metastases which represents major clinical hurdle in the prognosis and management of disease.A significant proportion ovarian cells peritoneal ascites exist as multicellular aggregates or spheroids.We hypothesize that these cellular spheroids are invasive with capacity to survive implant on surface.This study was designed elucidate early inherent mechanism(s) spheroid survival, growth disaggregation required for...